Tags

Type your tag names separated by a space and hit enter

DNA immunization with eukaryotic initiation factor-2α of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice.
Vaccine. 2013 Dec 16; 31(52):6225-31.V

Abstract

Toxoplasma gondii infection is a serious health problem of humans and animals worldwide. T. gondii eukaryotic initiation factor-2α (TgIF2α) plays a crucial role in parasite viability and is an important virulence factor of T. gondii. To evaluate the vaccine potential of TgIF2α, we constructed a novel eukaryotic plasmid pVAX-IF2α expressing TgIF2α from the RH strain and validated expression and immunogenicity in vitro in the Marc145 cell expression system by indirect immunofluorescence (IFA). Administration of pVAX-IF2α intramuscularly induced specific humoral immune responses including high levels of specific TgIF2α IgG antibody and a mixed IgG1/IgG2a response with a predominance of IgG2a production. The cellular immune response was elicited, showing significant production of IFN-γ and IL-2 associated with Th1 type response, and thus strong cell-mediated cytotoxic activity with increased frequencies of IFN-γ parameters analyzed in both CD4(+) and CD8(+) T cell compartments (CD4(+) IFN-γ(+) T cells and CD8(+) IFN-γ(+) T cells). Immunization resulted in partial protection against acute and chronic toxoplamosis in outbred Kunming mice, demonstrated by a significantly prolonged survival time (15.9±4.6 days) after challenge with the virulent RH strain and significant reduction in brain cysts (44.1%) against chronic infection with PRU cyst in contrast to control mice. Our data suggested that pVAX-IF2α could be used as a DNA vaccine candidate against both acute and chronic T. gondii infection by the activation of effective humoral and cellular immune responses.

Authors+Show Affiliations

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24183979

Citation

Chen, Jia, et al. "DNA Immunization With Eukaryotic Initiation Factor-2α of Toxoplasma Gondii Induces Protective Immunity Against Acute and Chronic Toxoplasmosis in Mice." Vaccine, vol. 31, no. 52, 2013, pp. 6225-31.
Chen J, Huang SY, Zhou DH, et al. DNA immunization with eukaryotic initiation factor-2α of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. Vaccine. 2013;31(52):6225-31.
Chen, J., Huang, S. Y., Zhou, D. H., Li, Z. Y., Petersen, E., Song, H. Q., & Zhu, X. Q. (2013). DNA immunization with eukaryotic initiation factor-2α of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. Vaccine, 31(52), 6225-31. https://doi.org/10.1016/j.vaccine.2013.10.034
Chen J, et al. DNA Immunization With Eukaryotic Initiation Factor-2α of Toxoplasma Gondii Induces Protective Immunity Against Acute and Chronic Toxoplasmosis in Mice. Vaccine. 2013 Dec 16;31(52):6225-31. PubMed PMID: 24183979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA immunization with eukaryotic initiation factor-2α of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. AU - Chen,Jia, AU - Huang,Si-Yang, AU - Zhou,Dong-Hui, AU - Li,Zhong-Yuan, AU - Petersen,Eskild, AU - Song,Hui-Qun, AU - Zhu,Xing-Quan, Y1 - 2013/10/31/ PY - 2013/07/06/received PY - 2013/09/20/revised PY - 2013/10/08/accepted PY - 2013/11/5/entrez PY - 2013/11/5/pubmed PY - 2014/7/16/medline KW - DNA vaccine KW - Eukaryotic initiation factor-2α (TgIF2α) KW - Mice KW - Protective immunity KW - Toxoplasma gondii KW - Toxoplasmosis SP - 6225 EP - 31 JF - Vaccine JO - Vaccine VL - 31 IS - 52 N2 - Toxoplasma gondii infection is a serious health problem of humans and animals worldwide. T. gondii eukaryotic initiation factor-2α (TgIF2α) plays a crucial role in parasite viability and is an important virulence factor of T. gondii. To evaluate the vaccine potential of TgIF2α, we constructed a novel eukaryotic plasmid pVAX-IF2α expressing TgIF2α from the RH strain and validated expression and immunogenicity in vitro in the Marc145 cell expression system by indirect immunofluorescence (IFA). Administration of pVAX-IF2α intramuscularly induced specific humoral immune responses including high levels of specific TgIF2α IgG antibody and a mixed IgG1/IgG2a response with a predominance of IgG2a production. The cellular immune response was elicited, showing significant production of IFN-γ and IL-2 associated with Th1 type response, and thus strong cell-mediated cytotoxic activity with increased frequencies of IFN-γ parameters analyzed in both CD4(+) and CD8(+) T cell compartments (CD4(+) IFN-γ(+) T cells and CD8(+) IFN-γ(+) T cells). Immunization resulted in partial protection against acute and chronic toxoplamosis in outbred Kunming mice, demonstrated by a significantly prolonged survival time (15.9±4.6 days) after challenge with the virulent RH strain and significant reduction in brain cysts (44.1%) against chronic infection with PRU cyst in contrast to control mice. Our data suggested that pVAX-IF2α could be used as a DNA vaccine candidate against both acute and chronic T. gondii infection by the activation of effective humoral and cellular immune responses. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/24183979/DNA_immunization_with_eukaryotic_initiation_factor_2α_of_Toxoplasma_gondii_induces_protective_immunity_against_acute_and_chronic_toxoplasmosis_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(13)01410-2 DB - PRIME DP - Unbound Medicine ER -