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Effects of globularifolin on cell survival, nuclear factor-κB activity, neopterin production, tryptophan breakdown and free radicals in vitro.
Fitoterapia. 2014 Jan; 92:85-92.F

Abstract

The potential effects of globularifolin, an acylated iridoid glucoside, on cell survival, inflammation markers and free radicals scavenging were investigated. Viability assay on human myelomomonocytic cell line THP-1 and human peripheral blood mononuclear cells (PBMC) using the Cell-Titer Blue assay proved that globularifolin had no toxic effect at the tested concentrations. Conversely, it is proportional to the dose globularifolin increased growth of THP-1 cells (p <0.01). On human PBMC, globularifolin at 6.25 and 12.5 μM concentrations showed a stimulatory effect, while at 12.5-200 μM it suppressed response of PBMC to stimulation with phytohemagglutinin (PHA). Globularifolin (50-200 μM) enhanced neopterin formation dose-dependently, whereas tryptophan breakdown was not influenced. At 50-200 μM in unstimulated PBMC in THP-1 cells, globularifolin induced a significant expression of nuclear factor-κB (NF-κB) as was quantified by Quanti-Blue assay. By contrast, in lipopolysaccharide (LPS)-stimulated cells, the higher concentrations of globularifolin suppressed NF-κB expression dose-dependently and a significant decrease was observed at 200 μM concentration. A positive correlation was found between increased neopterin and NF-κB activity (p <0.01). Similarly, a positive correlation was observed between neopterin levels in mitogen-induced cells and NF-κB activity in LPS-stimulated cells after treatment with globularifolin (p=0.001). The free radical scavenging capacity of globularifolin evaluated by Oxygen Radical Absorbance Capacity (ORAC) assay showed relative ORAC values of 0.36±0.05 μmol Trolox equivalent/μmol. All together, results show that natural antioxidant globularifolin might represent a potential immunomodulatory as well as proliferative agent, which deserves further in vitro and in vivo studies.

Authors+Show Affiliations

Department of Toxicology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul, Turkey; Divisions of Biological Chemistry, Biocenter, Medical University, Innsbruck, Austria.Medical Biochemistry, Biocenter, Medical University, Innsbruck, Austria.Medical Biochemistry, Biocenter, Medical University, Innsbruck, Austria.Department of Toxicology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul, Turkey.Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul, Turkey.Central Institute of Blood Transfusion and Immunology, University Clinics, Innsbruck, Austria.Department of Toxicology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul, Turkey.Medical Biochemistry, Biocenter, Medical University, Innsbruck, Austria. Electronic address: dietmar.fuchs@i-med.ac.at.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24185011

Citation

Sipahi, Hande, et al. "Effects of Globularifolin On Cell Survival, Nuclear factor-κB Activity, Neopterin Production, Tryptophan Breakdown and Free Radicals in Vitro." Fitoterapia, vol. 92, 2014, pp. 85-92.
Sipahi H, Becker K, Gostner JM, et al. Effects of globularifolin on cell survival, nuclear factor-κB activity, neopterin production, tryptophan breakdown and free radicals in vitro. Fitoterapia. 2014;92:85-92.
Sipahi, H., Becker, K., Gostner, J. M., Charehsaz, M., Kirmizibekmez, H., Schennach, H., Aydin, A., & Fuchs, D. (2014). Effects of globularifolin on cell survival, nuclear factor-κB activity, neopterin production, tryptophan breakdown and free radicals in vitro. Fitoterapia, 92, 85-92. https://doi.org/10.1016/j.fitote.2013.10.012
Sipahi H, et al. Effects of Globularifolin On Cell Survival, Nuclear factor-κB Activity, Neopterin Production, Tryptophan Breakdown and Free Radicals in Vitro. Fitoterapia. 2014;92:85-92. PubMed PMID: 24185011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of globularifolin on cell survival, nuclear factor-κB activity, neopterin production, tryptophan breakdown and free radicals in vitro. AU - Sipahi,Hande, AU - Becker,Kathrin, AU - Gostner,Johanna M, AU - Charehsaz,Mohammad, AU - Kirmizibekmez,Hasan, AU - Schennach,Harald, AU - Aydin,Ahmet, AU - Fuchs,Dietmar, Y1 - 2013/11/01/ PY - 2013/08/12/received PY - 2013/10/21/revised PY - 2013/10/25/accepted PY - 2013/11/5/entrez PY - 2013/11/5/pubmed PY - 2014/9/13/medline KW - 2,2′-azobis(2-amidinopropane) dihydrochloride KW - 6-hydroxy- 2,5,7,8-tetramethyl-2-carboxylic acid KW - AAPH KW - Antioxidant activity KW - CTB KW - Cell-Titer Blue® KW - Cytotoxicity KW - FCS KW - FL KW - Globularifolin KW - HAT KW - IDO KW - IFN-γ KW - Immunomodulatory KW - Immunosuppressive KW - Iridoid glycoside KW - NF-κB KW - ORAC KW - PBMC KW - PHA KW - SEAP KW - TNF-α KW - Trolox KW - fetal calf serum KW - fluorescein KW - hydrogen atom transfer KW - indoleamine (2,3)-dioxygenase KW - interferon gamma KW - kyn KW - kynurenine KW - neop KW - neopterin KW - nuclear factor-κB KW - oxygen radical absorbance capacity KW - peripheral blood mononuclear cells KW - phytohemagglutinin KW - secreted embryonic alkaline phosphatase KW - trp KW - tryptophan KW - tumor necrosis factor alpha SP - 85 EP - 92 JF - Fitoterapia JO - Fitoterapia VL - 92 N2 - The potential effects of globularifolin, an acylated iridoid glucoside, on cell survival, inflammation markers and free radicals scavenging were investigated. Viability assay on human myelomomonocytic cell line THP-1 and human peripheral blood mononuclear cells (PBMC) using the Cell-Titer Blue assay proved that globularifolin had no toxic effect at the tested concentrations. Conversely, it is proportional to the dose globularifolin increased growth of THP-1 cells (p <0.01). On human PBMC, globularifolin at 6.25 and 12.5 μM concentrations showed a stimulatory effect, while at 12.5-200 μM it suppressed response of PBMC to stimulation with phytohemagglutinin (PHA). Globularifolin (50-200 μM) enhanced neopterin formation dose-dependently, whereas tryptophan breakdown was not influenced. At 50-200 μM in unstimulated PBMC in THP-1 cells, globularifolin induced a significant expression of nuclear factor-κB (NF-κB) as was quantified by Quanti-Blue assay. By contrast, in lipopolysaccharide (LPS)-stimulated cells, the higher concentrations of globularifolin suppressed NF-κB expression dose-dependently and a significant decrease was observed at 200 μM concentration. A positive correlation was found between increased neopterin and NF-κB activity (p <0.01). Similarly, a positive correlation was observed between neopterin levels in mitogen-induced cells and NF-κB activity in LPS-stimulated cells after treatment with globularifolin (p=0.001). The free radical scavenging capacity of globularifolin evaluated by Oxygen Radical Absorbance Capacity (ORAC) assay showed relative ORAC values of 0.36±0.05 μmol Trolox equivalent/μmol. All together, results show that natural antioxidant globularifolin might represent a potential immunomodulatory as well as proliferative agent, which deserves further in vitro and in vivo studies. SN - 1873-6971 UR - https://www.unboundmedicine.com/medline/citation/24185011/Effects_of_globularifolin_on_cell_survival_nuclear_factor_κB_activity_neopterin_production_tryptophan_breakdown_and_free_radicals_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(13)00289-X DB - PRIME DP - Unbound Medicine ER -