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Physiological effects of nanoparticulate ZnO in green peas (Pisum sativum L.) cultivated in soil.
Metallomics. 2014 Jan; 6(1):132-8.M

Abstract

The toxicological effects of zinc oxide nanoparticles (ZnO NPs) in plants are still largely unknown. In the present study, green pea (Pisum sativum L.) plants were treated with 0, 125, 250, and 500 mg kg(-1) of either ZnO NPs or bulk ZnO in organic matter enriched soil. Corresponding toxicological effects were measured on the basis of plant growth, chlorophyll production, Zn bioaccumulation, H2O2 generation, stress enzyme activity, and lipid peroxidation using different cellular, molecular, and biochemical approaches. Compared to control, all ZnO NP concentrations significantly increased (p ≤ 0.05) root elongation but no effects were observed in the stem. Whereas all bulk ZnO treatments significantly increased both root and stem length. After 25 days, chlorophyll in leaves decreased, compared to control, by ~61%, 67%, and 77% in plants treated with 125, 250, and 500 mg kg(-1) ZnO NPs, respectively. Similar results were found in bulk ZnO treated plants. At all ZnO NP concentrations CAT was significantly reduced in leaves (p ≤ 0.05), while APOX was reduced in both roots and leaves. In the case of bulk ZnO, APOX activity was down-regulated in the root and leaf and CAT was unaffected. At 500 mg kg(-1) treatment, the H2O2 in leaves increased by 61% with a twofold lipid peroxidation, which would be a predictive biomarker of nanotoxicity. This study could be pioneering in evaluating the phytotoxicity of ZnO NPs to green peas and can serve as a good indicator for measuring the effects on ZnO NPs in plants grown in organic matter enriched soil.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Mukherjee A
Environmental Science and Engineering PhD program, The University of Texas at El Paso, 500 West University Ave., El Paso, TX 79968, USA. jgardea@utep.edu.
Peralta-Videa JR
No affiliation info available
Bandyopadhyay S
No affiliation info available
Rico CM
No affiliation info available
Zhao L
No affiliation info available
Gardea-Torresdey JL
No affiliation info available

MeSH

Ascorbate PeroxidasesCatalaseChlorophyllDose-Response Relationship, DrugHydrogen PeroxideLipid PeroxidationMetal NanoparticlesPeasPlant LeavesPlant RootsPlant StemsSoilTime FactorsZincZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24190632

Citation

Mukherjee, Arnab, et al. "Physiological Effects of Nanoparticulate ZnO in Green Peas (Pisum Sativum L.) Cultivated in Soil." Metallomics : Integrated Biometal Science, vol. 6, no. 1, 2014, pp. 132-8.
Mukherjee A, Peralta-Videa JR, Bandyopadhyay S, et al. Physiological effects of nanoparticulate ZnO in green peas (Pisum sativum L.) cultivated in soil. Metallomics. 2014;6(1):132-8.
Mukherjee, A., Peralta-Videa, J. R., Bandyopadhyay, S., Rico, C. M., Zhao, L., & Gardea-Torresdey, J. L. (2014). Physiological effects of nanoparticulate ZnO in green peas (Pisum sativum L.) cultivated in soil. Metallomics : Integrated Biometal Science, 6(1), 132-8. https://doi.org/10.1039/c3mt00064h
Mukherjee A, et al. Physiological Effects of Nanoparticulate ZnO in Green Peas (Pisum Sativum L.) Cultivated in Soil. Metallomics. 2014;6(1):132-8. PubMed PMID: 24190632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physiological effects of nanoparticulate ZnO in green peas (Pisum sativum L.) cultivated in soil. AU - Mukherjee,Arnab, AU - Peralta-Videa,Jose R, AU - Bandyopadhyay,Susmita, AU - Rico,Cyren M, AU - Zhao,Lijuan, AU - Gardea-Torresdey,Jorge L, PY - 2013/11/6/entrez PY - 2013/11/6/pubmed PY - 2014/8/15/medline SP - 132 EP - 8 JF - Metallomics : integrated biometal science JO - Metallomics VL - 6 IS - 1 N2 - The toxicological effects of zinc oxide nanoparticles (ZnO NPs) in plants are still largely unknown. In the present study, green pea (Pisum sativum L.) plants were treated with 0, 125, 250, and 500 mg kg(-1) of either ZnO NPs or bulk ZnO in organic matter enriched soil. Corresponding toxicological effects were measured on the basis of plant growth, chlorophyll production, Zn bioaccumulation, H2O2 generation, stress enzyme activity, and lipid peroxidation using different cellular, molecular, and biochemical approaches. Compared to control, all ZnO NP concentrations significantly increased (p ≤ 0.05) root elongation but no effects were observed in the stem. Whereas all bulk ZnO treatments significantly increased both root and stem length. After 25 days, chlorophyll in leaves decreased, compared to control, by ~61%, 67%, and 77% in plants treated with 125, 250, and 500 mg kg(-1) ZnO NPs, respectively. Similar results were found in bulk ZnO treated plants. At all ZnO NP concentrations CAT was significantly reduced in leaves (p ≤ 0.05), while APOX was reduced in both roots and leaves. In the case of bulk ZnO, APOX activity was down-regulated in the root and leaf and CAT was unaffected. At 500 mg kg(-1) treatment, the H2O2 in leaves increased by 61% with a twofold lipid peroxidation, which would be a predictive biomarker of nanotoxicity. This study could be pioneering in evaluating the phytotoxicity of ZnO NPs to green peas and can serve as a good indicator for measuring the effects on ZnO NPs in plants grown in organic matter enriched soil. SN - 1756-591X UR - https://www.unboundmedicine.com/medline/citation/24190632/Physiological_effects_of_nanoparticulate_ZnO_in_green_peas__Pisum_sativum_L___cultivated_in_soil_ DB - PRIME DP - Unbound Medicine ER -