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Effect of TMB-8 on histamine release from isolated rat mast cells.
Int Arch Allergy Appl Immunol. 1986; 79(3):253-8.IA

Abstract

TMB-8 was capable of complete inhibition of the histamine release induced by antigen, compound 48/80, and the ionophore A23187 (2 microM). The effective concentrations for 50% inhibition (IC50) were 1.2 X 10(-4) M for antigen, 1.6 X 10(-4) M for compound 48/80 both in the absence and in the presence of calcium, and 0.7 X 10(-4) M for the ionophore. The inhibitory action was not affected by increased calcium concentration in the medium from 1 to 2m M. The presence of glucose in the medium counteracted the inhibition by TMB-8 and, for low concentrations of the ionophore (0.25 microM), TMB-8 caused a pronounced enhancement of the histamine release. Our results indicate that TMB-8 primarily exerts its effects by interference with oxidative metabolism rather than by affecting the intracellular calcium availability.

Authors

No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2419254

Citation

Grossman, N. "Effect of TMB-8 On Histamine Release From Isolated Rat Mast Cells." International Archives of Allergy and Applied Immunology, vol. 79, no. 3, 1986, pp. 253-8.
Grossman N. Effect of TMB-8 on histamine release from isolated rat mast cells. Int Arch Allergy Appl Immunol. 1986;79(3):253-8.
Grossman, N. (1986). Effect of TMB-8 on histamine release from isolated rat mast cells. International Archives of Allergy and Applied Immunology, 79(3), 253-8.
Grossman N. Effect of TMB-8 On Histamine Release From Isolated Rat Mast Cells. Int Arch Allergy Appl Immunol. 1986;79(3):253-8. PubMed PMID: 2419254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of TMB-8 on histamine release from isolated rat mast cells. A1 - Grossman,N, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 253 EP - 8 JF - International archives of allergy and applied immunology JO - Int Arch Allergy Appl Immunol VL - 79 IS - 3 N2 - TMB-8 was capable of complete inhibition of the histamine release induced by antigen, compound 48/80, and the ionophore A23187 (2 microM). The effective concentrations for 50% inhibition (IC50) were 1.2 X 10(-4) M for antigen, 1.6 X 10(-4) M for compound 48/80 both in the absence and in the presence of calcium, and 0.7 X 10(-4) M for the ionophore. The inhibitory action was not affected by increased calcium concentration in the medium from 1 to 2m M. The presence of glucose in the medium counteracted the inhibition by TMB-8 and, for low concentrations of the ionophore (0.25 microM), TMB-8 caused a pronounced enhancement of the histamine release. Our results indicate that TMB-8 primarily exerts its effects by interference with oxidative metabolism rather than by affecting the intracellular calcium availability. SN - 0020-5915 UR - https://www.unboundmedicine.com/medline/citation/2419254/Effect_of_TMB_8_on_histamine_release_from_isolated_rat_mast_cells_ DB - PRIME DP - Unbound Medicine ER -