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Effect of TMB-8 on histamine release from isolated rat mast cells.
Int Arch Allergy Appl Immunol. 1986; 79(3):253-8.IA

Abstract

TMB-8 was capable of complete inhibition of the histamine release induced by antigen, compound 48/80, and the ionophore A23187 (2 microM). The effective concentrations for 50% inhibition (IC50) were 1.2 X 10(-4) M for antigen, 1.6 X 10(-4) M for compound 48/80 both in the absence and in the presence of calcium, and 0.7 X 10(-4) M for the ionophore. The inhibitory action was not affected by increased calcium concentration in the medium from 1 to 2m M. The presence of glucose in the medium counteracted the inhibition by TMB-8 and, for low concentrations of the ionophore (0.25 microM), TMB-8 caused a pronounced enhancement of the histamine release. Our results indicate that TMB-8 primarily exerts its effects by interference with oxidative metabolism rather than by affecting the intracellular calcium availability.

Authors

Grossman N
No affiliation info available

MeSH

AnimalsCalcimycinCalciumCalcium Channel BlockersDose-Response Relationship, DrugGallic AcidGlucoseHistamine ReleaseIn Vitro TechniquesMaleMast CellsRatsRats, Inbred Strainsp-Methoxy-N-methylphenethylamine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2419254

Citation

Grossman, N. "Effect of TMB-8 On Histamine Release From Isolated Rat Mast Cells." International Archives of Allergy and Applied Immunology, vol. 79, no. 3, 1986, pp. 253-8.
Grossman N. Effect of TMB-8 on histamine release from isolated rat mast cells. Int Arch Allergy Appl Immunol. 1986;79(3):253-8.
Grossman, N. (1986). Effect of TMB-8 on histamine release from isolated rat mast cells. International Archives of Allergy and Applied Immunology, 79(3), 253-8.
Grossman N. Effect of TMB-8 On Histamine Release From Isolated Rat Mast Cells. Int Arch Allergy Appl Immunol. 1986;79(3):253-8. PubMed PMID: 2419254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of TMB-8 on histamine release from isolated rat mast cells. A1 - Grossman,N, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 253 EP - 8 JF - International archives of allergy and applied immunology JO - Int Arch Allergy Appl Immunol VL - 79 IS - 3 N2 - TMB-8 was capable of complete inhibition of the histamine release induced by antigen, compound 48/80, and the ionophore A23187 (2 microM). The effective concentrations for 50% inhibition (IC50) were 1.2 X 10(-4) M for antigen, 1.6 X 10(-4) M for compound 48/80 both in the absence and in the presence of calcium, and 0.7 X 10(-4) M for the ionophore. The inhibitory action was not affected by increased calcium concentration in the medium from 1 to 2m M. The presence of glucose in the medium counteracted the inhibition by TMB-8 and, for low concentrations of the ionophore (0.25 microM), TMB-8 caused a pronounced enhancement of the histamine release. Our results indicate that TMB-8 primarily exerts its effects by interference with oxidative metabolism rather than by affecting the intracellular calcium availability. SN - 0020-5915 UR - https://www.unboundmedicine.com/medline/citation/2419254/Effect_of_TMB_8_on_histamine_release_from_isolated_rat_mast_cells_ DB - PRIME DP - Unbound Medicine ER -