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Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies.
Fish Physiol Biochem 1995; 14(6):489-500FP

Abstract

Plasma estradiol-17β (E2), testosterone (T), 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and 17α,20β,21-tri-hydroxy-4-pregnen-3-one (20β-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20β-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E2 and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20β-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E2 and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20β-S and oocyte GVBD by white perch follices. DHP and 20β-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20β-S in the control of FOM in white perch and white bass.

Authors+Show Affiliations

Department of Zoology, North Carolina State University, Campus Box 7617, Raleigh, North Carolina, 27695-7617, U.S.A..No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24197645

Citation

King, W, et al. "Involvement of Gonadal Steroids in Final Oocyte Maturation of White Perch (Morone Americana) and White Bass (M. Chrysops): in Vivo and in Vitro Studies." Fish Physiology and Biochemistry, vol. 14, no. 6, 1995, pp. 489-500.
King W, Berlinsky DL, Sullivan CV. Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies. Fish Physiol Biochem. 1995;14(6):489-500.
King, W., Berlinsky, D. L., & Sullivan, C. V. (1995). Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies. Fish Physiology and Biochemistry, 14(6), pp. 489-500. doi:10.1007/BF00004349.
King W, Berlinsky DL, Sullivan CV. Involvement of Gonadal Steroids in Final Oocyte Maturation of White Perch (Morone Americana) and White Bass (M. Chrysops): in Vivo and in Vitro Studies. Fish Physiol Biochem. 1995;14(6):489-500. PubMed PMID: 24197645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies. AU - King,W,5th AU - Berlinsky,D L, AU - Sullivan,C V, PY - 1995/06/04/accepted PY - 2013/11/8/entrez PY - 1995/12/1/pubmed PY - 1995/12/1/medline SP - 489 EP - 500 JF - Fish physiology and biochemistry JO - Fish Physiol. Biochem. VL - 14 IS - 6 N2 - Plasma estradiol-17β (E2), testosterone (T), 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and 17α,20β,21-tri-hydroxy-4-pregnen-3-one (20β-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20β-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E2 and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20β-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E2 and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20β-S and oocyte GVBD by white perch follices. DHP and 20β-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20β-S in the control of FOM in white perch and white bass. SN - 0920-1742 UR - https://www.unboundmedicine.com/medline/citation/24197645/Involvement_of_gonadal_steroids_in_final_oocyte_maturation_of_white_perch__Morone_americana__and_white_bass__M__chrysops_:_in_vivo_and_in_vitro_studies_ DB - PRIME DP - Unbound Medicine ER -