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Genuine traditional Korean medicine, Naju Jjok (Chung-Dae, Polygonum tinctorium) improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesional skin.
Phytomedicine. 2014 Mar 15; 21(4):453-60.P

Abstract

PURPOSE

Naju Jjok (NJJ, Polygonum tinctorium) is a clear heat and release toxin medicinal. It has been used to treat various inflammatory diseases and as a dye in clothing in traditional Korean medicine. However, the effect of NJJ on atopic dermatitis (AD) has not been elucidated. Therefore, we examined whether NJJ would have an inhibitory effect on AD using the mimic AD murine model and in vitro model.

METHODS

We treated NJJ on 2,4-dinitrofluorobenzene (DNFB)-induced AD-like skin lesions in NC/Nga mice, phorbol myristate acetate/calcium ionophore A23187-stimulated human mast cell line (HMC-1) cells, and anti-CD3/anti-CD28-stimulated splenocytes. Histological analysis, ELISA, PCR, and Western blot analysis were performed.

RESULTS

The oral administration with NJJ suppressed the total clinical severity in DNFB-induced AD-like lesional skin. NJJ significantly suppressed the levels of inflammatory mRNA and protein in AD-like lesional skin. NJJ significantly suppressed the levels of IgE and interleukin-4 in the serum of DNFB-induced AD mice. The expression of mast cells-derived caspase-1 was suppressed by NJJ in AD-like lesional skin. In addition, topical application with NJJ improved clinical symptoms in DNFB-induced AD mice. The topical application with NJJ significantly suppressed the levels of IgE and histamine in the serum of DNFB-induced AD mice. NJJ suppressed the production and mRNA expression of TSLP by blockade of caspase-1 signal pathway in the activated HMC-1 cells. Furthermore, NJJ significantly decreased the production of tumor necrosis factor-α from the stimulated splenocytes.

CONCLUSIONS

In conclusion, these results propose curative potential of natural dye, NJJ by showing the scientific evidence on anti-AD effect of NJJ which has been used traditionally.

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Authors+Show Affiliations

Han NR
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Kang SW
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Moon PD
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
Jang JB
Regional Innovation Center and Inflammatory Disease Research Center, Hoseo University, 165, Sechul-ri, Baebang-myun, Asan, Chungnam 336-795, Republic of Korea; Biochip Research Center and Inflammatory Disease Research Center, Hoseo University, Asan, Chungnam 336-795, Republic of Korea.
Kim HM
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: hmkim@khu.ac.kr.
Jeong HJ
Biochip Research Center and Inflammatory Disease Research Center, Hoseo University, Asan, Chungnam 336-795, Republic of Korea. Electronic address: hjjeong@hoseo.edu.

MeSH

Administration, OralAnimalsCalcimycinCaspase 1Cell LineCytokinesDermatitis, AtopicDinitrofluorobenzeneDrug Evaluation, PreclinicalHumansMaleMedicine, Korean TraditionalMicePhytotherapyPlant ExtractsPolygonumReceptor-Interacting Protein Serine-Threonine Kinase 2Tetradecanoylphorbol AcetateTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24200496

Citation

Han, Na-Ra, et al. "Genuine Traditional Korean Medicine, Naju Jjok (Chung-Dae, Polygonum Tinctorium) Improves 2,4-dinitrofluorobenzene-induced Atopic Dermatitis-like Lesional Skin." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 21, no. 4, 2014, pp. 453-60.
Han NR, Kang SW, Moon PD, et al. Genuine traditional Korean medicine, Naju Jjok (Chung-Dae, Polygonum tinctorium) improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesional skin. Phytomedicine. 2014;21(4):453-60.
Han, N. R., Kang, S. W., Moon, P. D., Jang, J. B., Kim, H. M., & Jeong, H. J. (2014). Genuine traditional Korean medicine, Naju Jjok (Chung-Dae, Polygonum tinctorium) improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesional skin. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 21(4), 453-60. https://doi.org/10.1016/j.phymed.2013.09.021
Han NR, et al. Genuine Traditional Korean Medicine, Naju Jjok (Chung-Dae, Polygonum Tinctorium) Improves 2,4-dinitrofluorobenzene-induced Atopic Dermatitis-like Lesional Skin. Phytomedicine. 2014 Mar 15;21(4):453-60. PubMed PMID: 24200496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genuine traditional Korean medicine, Naju Jjok (Chung-Dae, Polygonum tinctorium) improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like lesional skin. AU - Han,Na-Ra, AU - Kang,Sang Woo, AU - Moon,Phil-Dong, AU - Jang,Jae-Bum, AU - Kim,Hyung-Min, AU - Jeong,Hyun-Ja, Y1 - 2013/11/04/ PY - 2013/07/22/received PY - 2013/08/23/revised PY - 2013/09/29/accepted PY - 2013/11/9/entrez PY - 2013/11/10/pubmed PY - 2014/12/17/medline KW - Atopic dermatitis KW - Caspase-1 KW - Mast cell KW - NC/Nga mice KW - Naju Jjok KW - TSLP SP - 453 EP - 60 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 21 IS - 4 N2 - PURPOSE: Naju Jjok (NJJ, Polygonum tinctorium) is a clear heat and release toxin medicinal. It has been used to treat various inflammatory diseases and as a dye in clothing in traditional Korean medicine. However, the effect of NJJ on atopic dermatitis (AD) has not been elucidated. Therefore, we examined whether NJJ would have an inhibitory effect on AD using the mimic AD murine model and in vitro model. METHODS: We treated NJJ on 2,4-dinitrofluorobenzene (DNFB)-induced AD-like skin lesions in NC/Nga mice, phorbol myristate acetate/calcium ionophore A23187-stimulated human mast cell line (HMC-1) cells, and anti-CD3/anti-CD28-stimulated splenocytes. Histological analysis, ELISA, PCR, and Western blot analysis were performed. RESULTS: The oral administration with NJJ suppressed the total clinical severity in DNFB-induced AD-like lesional skin. NJJ significantly suppressed the levels of inflammatory mRNA and protein in AD-like lesional skin. NJJ significantly suppressed the levels of IgE and interleukin-4 in the serum of DNFB-induced AD mice. The expression of mast cells-derived caspase-1 was suppressed by NJJ in AD-like lesional skin. In addition, topical application with NJJ improved clinical symptoms in DNFB-induced AD mice. The topical application with NJJ significantly suppressed the levels of IgE and histamine in the serum of DNFB-induced AD mice. NJJ suppressed the production and mRNA expression of TSLP by blockade of caspase-1 signal pathway in the activated HMC-1 cells. Furthermore, NJJ significantly decreased the production of tumor necrosis factor-α from the stimulated splenocytes. CONCLUSIONS: In conclusion, these results propose curative potential of natural dye, NJJ by showing the scientific evidence on anti-AD effect of NJJ which has been used traditionally. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/24200496/Genuine_traditional_Korean_medicine_Naju_Jjok__Chung_Dae_Polygonum_tinctorium__improves_24_dinitrofluorobenzene_induced_atopic_dermatitis_like_lesional_skin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(13)00408-X DB - PRIME DP - Unbound Medicine ER -