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Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint.
Anal Bioanal Chem. 2013 Dec; 405(30):9791-803.AB

Abstract

A cross-over controlled administration study of smoked cannabis was carried out on occasional and heavy smokers. The participants smoked a joint (11% Δ9-tetrahydrocannabinol (THC)) or a matching placebo on two different occasions. Whole blood (WB) and oral fluid (OF) samples were collected before and up to 3.5 h after smoking the joints. Pharmacokinetic analyses were obtained from these data. Questionnaires assessing the subjective effects were administered to the subjects during each session before and after the smoking time period. THC, 11-hydroxy-THC (11-OH-THC) and 11-nor-9-carboxy-THC (THCCOOH) were analyzed in the blood by gas chromatography or liquid chromatography (LC)-tandem mass spectrometry (MS/MS). The determination of THC, THCCOOH, cannabinol (CBN), and Δ9-tetrahydrocannabinolic acid A (THC-A) was carried out on OF only using LC-MS/MS. In line with the widely accepted assumption that cannabis smoking results in a strong contamination of the oral cavity, we found that THC, and also THC-A, shows a sharp, high concentration peak just after smoking, with a rapid decrease in these levels within 3 h. No obvious differences were found between both groups concerning THC median maximum concentrations measured either in blood or in OF; these levels were equal to 1,338 and 1,041 μg/L in OF and to 82 and 94 μg/L in WB for occasional and heavy smokers, respectively. The initial WB THCCOOH concentration was much higher in regular smokers than in occasional users. Compared with the occasional smokers, the sensation of confusion felt by the regular smokers was much less while the feeling of intoxication remained almost unchanged.

Authors+Show Affiliations

UTCF (Forensic Toxicology and Chemistry Unit), CURML (University Center of Legal Medicine), Rue du Bugnon 21, 1011, Lausanne, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24202191

Citation

Fabritius, Marie, et al. "Comparison of Cannabinoid Concentrations in Oral Fluid and Whole Blood Between Occasional and Regular Cannabis Smokers Prior to and After Smoking a Cannabis Joint." Analytical and Bioanalytical Chemistry, vol. 405, no. 30, 2013, pp. 9791-803.
Fabritius M, Chtioui H, Battistella G, et al. Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint. Anal Bioanal Chem. 2013;405(30):9791-803.
Fabritius, M., Chtioui, H., Battistella, G., Annoni, J. M., Dao, K., Favrat, B., Fornari, E., Lauer, E., Maeder, P., & Giroud, C. (2013). Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint. Analytical and Bioanalytical Chemistry, 405(30), 9791-803. https://doi.org/10.1007/s00216-013-7412-1
Fabritius M, et al. Comparison of Cannabinoid Concentrations in Oral Fluid and Whole Blood Between Occasional and Regular Cannabis Smokers Prior to and After Smoking a Cannabis Joint. Anal Bioanal Chem. 2013;405(30):9791-803. PubMed PMID: 24202191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint. AU - Fabritius,Marie, AU - Chtioui,Haithem, AU - Battistella,Giovanni, AU - Annoni,Jean-Marie, AU - Dao,Kim, AU - Favrat,Bernard, AU - Fornari,Eleonora, AU - Lauer,Estelle, AU - Maeder,Philippe, AU - Giroud,Christian, Y1 - 2013/11/08/ PY - 2013/07/18/received PY - 2013/10/02/accepted PY - 2013/09/26/revised PY - 2013/11/9/entrez PY - 2013/11/10/pubmed PY - 2014/6/28/medline SP - 9791 EP - 803 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 405 IS - 30 N2 - A cross-over controlled administration study of smoked cannabis was carried out on occasional and heavy smokers. The participants smoked a joint (11% Δ9-tetrahydrocannabinol (THC)) or a matching placebo on two different occasions. Whole blood (WB) and oral fluid (OF) samples were collected before and up to 3.5 h after smoking the joints. Pharmacokinetic analyses were obtained from these data. Questionnaires assessing the subjective effects were administered to the subjects during each session before and after the smoking time period. THC, 11-hydroxy-THC (11-OH-THC) and 11-nor-9-carboxy-THC (THCCOOH) were analyzed in the blood by gas chromatography or liquid chromatography (LC)-tandem mass spectrometry (MS/MS). The determination of THC, THCCOOH, cannabinol (CBN), and Δ9-tetrahydrocannabinolic acid A (THC-A) was carried out on OF only using LC-MS/MS. In line with the widely accepted assumption that cannabis smoking results in a strong contamination of the oral cavity, we found that THC, and also THC-A, shows a sharp, high concentration peak just after smoking, with a rapid decrease in these levels within 3 h. No obvious differences were found between both groups concerning THC median maximum concentrations measured either in blood or in OF; these levels were equal to 1,338 and 1,041 μg/L in OF and to 82 and 94 μg/L in WB for occasional and heavy smokers, respectively. The initial WB THCCOOH concentration was much higher in regular smokers than in occasional users. Compared with the occasional smokers, the sensation of confusion felt by the regular smokers was much less while the feeling of intoxication remained almost unchanged. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/24202191/Comparison_of_cannabinoid_concentrations_in_oral_fluid_and_whole_blood_between_occasional_and_regular_cannabis_smokers_prior_to_and_after_smoking_a_cannabis_joint_ L2 - https://dx.doi.org/10.1007/s00216-013-7412-1 DB - PRIME DP - Unbound Medicine ER -