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The synthesis of magnetic lysozyme-imprinted polymers by means of distillation-precipitation polymerization for selective protein enrichment.
Chem Asian J 2014; 9(2):526-33CA

Abstract

A protein imprinting approach for the synthesis of core-shell structure nanoparticles with a magnetic core and molecularly imprinted polymer (MIP) shell was developed using a simple distillation-precipitation polymerization method. In this work, Fe3O4 magnetic nanoparticles were first synthesized through a solvothermal method and then were conveniently surface-modified with 3-(methacryloyloxy)propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high-density MIP shell was coated onto the surface of the magnetic nanoparticles by the copolymerization of functional monomer acrylamide (AAm), cross-linking agent N,N'-methylenebisacrylamide (MBA), the initiator azodiisobutyronitrile (AIBN), and protein in acetonitrile heated at reflux. The morphology, adsorption, and recognition properties of the magnetic molecularly imprinted nanoparticles were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and rebinding experiments. The resulting MIP showed a high adsorption capacity (104.8 mg g(-1)) and specific recognition (imprinting factor=7.6) to lysozyme (Lyz). The as-prepared Fe3O4@Lyz-MIP nanoparticles with a mean diameter of 320 nm were coated with an MIP shell that was 20 nm thick, which enabled Fe3O4@Lyz-MIP to easily reach adsorption equilibrium. The high magnetization saturation (40.35 emu g(-1)) endows the materials with the convenience of magnetic separation under an external magnetic field and allows them to be subsequently reused. Furthermore, Fe3O4@Lyz-MIP could selectively extract a target protein from real egg-white samples under an external magnetic field.

Authors+Show Affiliations

State Key Laboratory of Medical Chemical Biology, Department of Chemistry, Nankai University, Tianjin 300071 (China), Fax: (+86) 022-23502458; Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071 (China).No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24203562

Citation

Cao, Jiali, et al. "The Synthesis of Magnetic Lysozyme-imprinted Polymers By Means of Distillation-precipitation Polymerization for Selective Protein Enrichment." Chemistry, an Asian Journal, vol. 9, no. 2, 2014, pp. 526-33.
Cao J, Zhang X, He X, et al. The synthesis of magnetic lysozyme-imprinted polymers by means of distillation-precipitation polymerization for selective protein enrichment. Chem Asian J. 2014;9(2):526-33.
Cao, J., Zhang, X., He, X., Chen, L., & Zhang, Y. (2014). The synthesis of magnetic lysozyme-imprinted polymers by means of distillation-precipitation polymerization for selective protein enrichment. Chemistry, an Asian Journal, 9(2), pp. 526-33. doi:10.1002/asia.201300937.
Cao J, et al. The Synthesis of Magnetic Lysozyme-imprinted Polymers By Means of Distillation-precipitation Polymerization for Selective Protein Enrichment. Chem Asian J. 2014;9(2):526-33. PubMed PMID: 24203562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The synthesis of magnetic lysozyme-imprinted polymers by means of distillation-precipitation polymerization for selective protein enrichment. AU - Cao,Jiali, AU - Zhang,Xihao, AU - He,Xiwen, AU - Chen,Langxing, AU - Zhang,Yukui, Y1 - 2013/11/06/ PY - 2013/07/16/received PY - 2013/08/21/revised PY - 2013/11/9/entrez PY - 2013/11/10/pubmed PY - 2015/9/9/medline KW - imprinting KW - magnetic properties KW - nanoparticles KW - polymerization KW - proteins SP - 526 EP - 33 JF - Chemistry, an Asian journal JO - Chem Asian J VL - 9 IS - 2 N2 - A protein imprinting approach for the synthesis of core-shell structure nanoparticles with a magnetic core and molecularly imprinted polymer (MIP) shell was developed using a simple distillation-precipitation polymerization method. In this work, Fe3O4 magnetic nanoparticles were first synthesized through a solvothermal method and then were conveniently surface-modified with 3-(methacryloyloxy)propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high-density MIP shell was coated onto the surface of the magnetic nanoparticles by the copolymerization of functional monomer acrylamide (AAm), cross-linking agent N,N'-methylenebisacrylamide (MBA), the initiator azodiisobutyronitrile (AIBN), and protein in acetonitrile heated at reflux. The morphology, adsorption, and recognition properties of the magnetic molecularly imprinted nanoparticles were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and rebinding experiments. The resulting MIP showed a high adsorption capacity (104.8 mg g(-1)) and specific recognition (imprinting factor=7.6) to lysozyme (Lyz). The as-prepared Fe3O4@Lyz-MIP nanoparticles with a mean diameter of 320 nm were coated with an MIP shell that was 20 nm thick, which enabled Fe3O4@Lyz-MIP to easily reach adsorption equilibrium. The high magnetization saturation (40.35 emu g(-1)) endows the materials with the convenience of magnetic separation under an external magnetic field and allows them to be subsequently reused. Furthermore, Fe3O4@Lyz-MIP could selectively extract a target protein from real egg-white samples under an external magnetic field. SN - 1861-471X UR - https://www.unboundmedicine.com/medline/citation/24203562/The_synthesis_of_magnetic_lysozyme_imprinted_polymers_by_means_of_distillation_precipitation_polymerization_for_selective_protein_enrichment_ L2 - https://doi.org/10.1002/asia.201300937 DB - PRIME DP - Unbound Medicine ER -