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Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications.
Int J Nanomedicine 2013; 8:4197-213IJ

Abstract

The regeneration of functional tissue in osseous defects is a formidable challenge in orthopedic surgery. In the present study, a novel biomimetic composite scaffold, here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated using a selective laser sintering technique. The macrostructure, morphology, and mechanical strength of the scaffolds were characterized. Scanning electronic microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned, well-ordered macropores and interconnected micropores. The scaffolds have a range of porosity from 78.54% to 70.31%, and a corresponding compressive strength of 1.38 MPa to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL or PCL scaffolds and cultured for 28 days in vitro. As indicated by the level of cell attachment and proliferation, the nano-HA/PCL showed excellent biocompatibility, comparable to that of PCL scaffolds. The hydrophilicity, mineralization, alkaline phosphatase activity, and Alizarin Red S staining indicated that the nano-HA/PCL scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics showed that after nano-HA was added, the material increased the rate of rhBMP-2 release. To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in rabbit femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically and histologically. The in vivo results showed that both nano-HA/PCL composite scaffolds and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL scaffolds enhanced the efficiency of new bone formation more than PCL scaffolds and fulfilled all the basic requirements of bone tissue engineering scaffolds. Thus, they show large potential for use in orthopedic and reconstructive surgery.

Authors+Show Affiliations

Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24204147

Citation

Xia, Yan, et al. "Selective Laser Sintering Fabrication of Nano-hydroxyapatite/poly-ε-caprolactone Scaffolds for Bone Tissue Engineering Applications." International Journal of Nanomedicine, vol. 8, 2013, pp. 4197-213.
Xia Y, Zhou P, Cheng X, et al. Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications. Int J Nanomedicine. 2013;8:4197-213.
Xia, Y., Zhou, P., Cheng, X., Xie, Y., Liang, C., Li, C., & Xu, S. (2013). Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications. International Journal of Nanomedicine, 8, pp. 4197-213. doi:10.2147/IJN.S50685.
Xia Y, et al. Selective Laser Sintering Fabrication of Nano-hydroxyapatite/poly-ε-caprolactone Scaffolds for Bone Tissue Engineering Applications. Int J Nanomedicine. 2013;8:4197-213. PubMed PMID: 24204147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications. AU - Xia,Yan, AU - Zhou,Panyu, AU - Cheng,Xiaosong, AU - Xie,Yang, AU - Liang,Chong, AU - Li,Chao, AU - Xu,Shuogui, Y1 - 2013/11/01/ PY - 2013/11/9/entrez PY - 2013/11/10/pubmed PY - 2014/6/10/medline KW - biomimetic composite scaffold KW - orthopedic surgery KW - osseous defects KW - reconstructive surgery SP - 4197 EP - 213 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 8 N2 - The regeneration of functional tissue in osseous defects is a formidable challenge in orthopedic surgery. In the present study, a novel biomimetic composite scaffold, here called nano-hydroxyapatite (HA)/poly-ε-caprolactone (PCL) was fabricated using a selective laser sintering technique. The macrostructure, morphology, and mechanical strength of the scaffolds were characterized. Scanning electronic microscopy (SEM) showed that the nano-HA/PCL scaffolds exhibited predesigned, well-ordered macropores and interconnected micropores. The scaffolds have a range of porosity from 78.54% to 70.31%, and a corresponding compressive strength of 1.38 MPa to 3.17 MPa. Human bone marrow stromal cells were seeded onto the nano-HA/PCL or PCL scaffolds and cultured for 28 days in vitro. As indicated by the level of cell attachment and proliferation, the nano-HA/PCL showed excellent biocompatibility, comparable to that of PCL scaffolds. The hydrophilicity, mineralization, alkaline phosphatase activity, and Alizarin Red S staining indicated that the nano-HA/PCL scaffolds are more bioactive than the PCL scaffolds in vitro. Measurements of recombinant human bone morphogenetic protein-2 (rhBMP-2) release kinetics showed that after nano-HA was added, the material increased the rate of rhBMP-2 release. To investigate the in vivo biocompatibility and osteogenesis of the composite scaffolds, both nano-HA/PCL scaffolds and PCL scaffolds were implanted in rabbit femur defects for 3, 6, and 9 weeks. The wounds were studied radiographically and histologically. The in vivo results showed that both nano-HA/PCL composite scaffolds and PCL scaffolds exhibited good biocompatibility. However, the nano-HA/PCL scaffolds enhanced the efficiency of new bone formation more than PCL scaffolds and fulfilled all the basic requirements of bone tissue engineering scaffolds. Thus, they show large potential for use in orthopedic and reconstructive surgery. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/24204147/Selective_laser_sintering_fabrication_of_nano_hydroxyapatite/poly_ε_caprolactone_scaffolds_for_bone_tissue_engineering_applications_ L2 - https://dx.doi.org/10.2147/IJN.S50685 DB - PRIME DP - Unbound Medicine ER -