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Population structure analyses of Staphylococcus aureus at Tygerberg Hospital, South Africa, reveals a diverse population, a high prevalence of Panton-Valentine leukocidin genes, and unique local methicillin-resistant S. aureus clones.
Clin Microbiol Infect. 2014 Jul; 20(7):652-9.CM

Abstract

Studies reporting on the population structure of Staphylococcus aureus in South Africa have focused only on methicillin-resistant S. aureus (MRSA). This study describes the population structure of S. aureus, including methicillin-susceptible S. aureus (MSSA) isolated from patients at Tygerberg Academic Hospital, Western Cape province. Pulsed-field gel electrophoresis (PFGE), detection of Panton-Valentine leukocidin (PVL), spa typing, multilocus sequence typing (MLST), agr typing and SCCmec typing were used to characterize strains. Of 367 non-repetitive S. aureus isolates collected over a period of 1 year, 56 (15.3%) were MRSA. Skin and soft tissue infections were the most frequent source (54.8%), followed by bone and joint (15.3%) and respiratory tract infections (7.7%). For strain typing, PFGE was the most discriminative method, and resulted in 31 pulsotypes (n = 345, 94.0%), as compared with 16 spa clonal complexes (CCs) (n = 344, 93.4%). Four MLST CCs were identified after eBURST of sequence types (STs) of selected isolates. One hundred and sixty isolates (MSSA, n = 155, 42.2%) were PVL-positive, and agr types I-IV and SCCmec types I-V were identified. Our S. aureus population consisted of genotypically diverse strains, with PVL being a common characteristic of MSSA. MSSA and MRSA isolates clustered in different clones. However, the dominant MRSA clone (ST612) also contained an MSSA isolate, and had a unique genotype. Common global epidemic MRSA clones, such as ST239-MRSA-III and ST36-MRSA-II, were identified. A local clone, ST612-MRSA-IV, was found to be the dominant MRSA clone.

Authors+Show Affiliations

Division of Medical Microbiology, Tygerberg Hospital, Stellenbosch University, Cape Town, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24206111

Citation

Oosthuysen, W F., et al. "Population Structure Analyses of Staphylococcus Aureus at Tygerberg Hospital, South Africa, Reveals a Diverse Population, a High Prevalence of Panton-Valentine Leukocidin Genes, and Unique Local Methicillin-resistant S. Aureus Clones." Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, vol. 20, no. 7, 2014, pp. 652-9.
Oosthuysen WF, Orth H, Lombard CJ, et al. Population structure analyses of Staphylococcus aureus at Tygerberg Hospital, South Africa, reveals a diverse population, a high prevalence of Panton-Valentine leukocidin genes, and unique local methicillin-resistant S. aureus clones. Clin Microbiol Infect. 2014;20(7):652-9.
Oosthuysen, W. F., Orth, H., Lombard, C. J., Sinha, B., & Wasserman, E. (2014). Population structure analyses of Staphylococcus aureus at Tygerberg Hospital, South Africa, reveals a diverse population, a high prevalence of Panton-Valentine leukocidin genes, and unique local methicillin-resistant S. aureus clones. Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 20(7), 652-9. https://doi.org/10.1111/1469-0691.12452
Oosthuysen WF, et al. Population Structure Analyses of Staphylococcus Aureus at Tygerberg Hospital, South Africa, Reveals a Diverse Population, a High Prevalence of Panton-Valentine Leukocidin Genes, and Unique Local Methicillin-resistant S. Aureus Clones. Clin Microbiol Infect. 2014;20(7):652-9. PubMed PMID: 24206111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Population structure analyses of Staphylococcus aureus at Tygerberg Hospital, South Africa, reveals a diverse population, a high prevalence of Panton-Valentine leukocidin genes, and unique local methicillin-resistant S. aureus clones. AU - Oosthuysen,W F, AU - Orth,H, AU - Lombard,C J, AU - Sinha,B, AU - Wasserman,E, Y1 - 2013/12/09/ PY - 2013/06/14/received PY - 2013/11/06/revised PY - 2013/11/07/accepted PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2015/3/31/medline KW - Methicillin resistance KW - PVL KW - S. aureus KW - SCC mec KW - South Africa SP - 652 EP - 9 JF - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JO - Clin Microbiol Infect VL - 20 IS - 7 N2 - Studies reporting on the population structure of Staphylococcus aureus in South Africa have focused only on methicillin-resistant S. aureus (MRSA). This study describes the population structure of S. aureus, including methicillin-susceptible S. aureus (MSSA) isolated from patients at Tygerberg Academic Hospital, Western Cape province. Pulsed-field gel electrophoresis (PFGE), detection of Panton-Valentine leukocidin (PVL), spa typing, multilocus sequence typing (MLST), agr typing and SCCmec typing were used to characterize strains. Of 367 non-repetitive S. aureus isolates collected over a period of 1 year, 56 (15.3%) were MRSA. Skin and soft tissue infections were the most frequent source (54.8%), followed by bone and joint (15.3%) and respiratory tract infections (7.7%). For strain typing, PFGE was the most discriminative method, and resulted in 31 pulsotypes (n = 345, 94.0%), as compared with 16 spa clonal complexes (CCs) (n = 344, 93.4%). Four MLST CCs were identified after eBURST of sequence types (STs) of selected isolates. One hundred and sixty isolates (MSSA, n = 155, 42.2%) were PVL-positive, and agr types I-IV and SCCmec types I-V were identified. Our S. aureus population consisted of genotypically diverse strains, with PVL being a common characteristic of MSSA. MSSA and MRSA isolates clustered in different clones. However, the dominant MRSA clone (ST612) also contained an MSSA isolate, and had a unique genotype. Common global epidemic MRSA clones, such as ST239-MRSA-III and ST36-MRSA-II, were identified. A local clone, ST612-MRSA-IV, was found to be the dominant MRSA clone. SN - 1469-0691 UR - https://www.unboundmedicine.com/medline/citation/24206111/Population_structure_analyses_of_Staphylococcus_aureus_at_Tygerberg_Hospital_South_Africa_reveals_a_diverse_population_a_high_prevalence_of_Panton_Valentine_leukocidin_genes_and_unique_local_methicillin_resistant_S__aureus_clones_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(14)61152-6 DB - PRIME DP - Unbound Medicine ER -