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Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column.
Food Chem. 2014 Mar 15; 147:262-8.FC

Abstract

A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4 mL min(-1), the catechins and caffeine were isocratically separated in about 7 min. The limits of detection and quantification were in the range of 0.11-0.29 and 0.33-0.87 mg L(-1), respectively. Satisfactory recoveries were obtained (94.2-105.2 ± 1.8%) for all samples when spiked at three concentrations (5, 40 and 70 mg L(-1)). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas.

Authors+Show Affiliations

School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: afidah@usm.my.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24206716

Citation

Rahim, A A., et al. "Rapid Tea Catechins and Caffeine Determination By HPLC Using Microwave-assisted Extraction and Silica Monolithic Column." Food Chemistry, vol. 147, 2014, pp. 262-8.
Rahim AA, Nofrizal S, Saad B. Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column. Food Chem. 2014;147:262-8.
Rahim, A. A., Nofrizal, S., & Saad, B. (2014). Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column. Food Chemistry, 147, 262-8. https://doi.org/10.1016/j.foodchem.2013.09.131
Rahim AA, Nofrizal S, Saad B. Rapid Tea Catechins and Caffeine Determination By HPLC Using Microwave-assisted Extraction and Silica Monolithic Column. Food Chem. 2014 Mar 15;147:262-8. PubMed PMID: 24206716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column. AU - Rahim,A A, AU - Nofrizal,S, AU - Saad,Bahruddin, Y1 - 2013/10/03/ PY - 2011/10/11/received PY - 2013/09/11/revised PY - 2013/09/25/accepted PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2014/6/11/medline KW - Black tea KW - Catechin KW - Green tea KW - High performance liquid chromatography KW - Monolithic column KW - Oolong tea SP - 262 EP - 8 JF - Food chemistry JO - Food Chem VL - 147 N2 - A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4 mL min(-1), the catechins and caffeine were isocratically separated in about 7 min. The limits of detection and quantification were in the range of 0.11-0.29 and 0.33-0.87 mg L(-1), respectively. Satisfactory recoveries were obtained (94.2-105.2 ± 1.8%) for all samples when spiked at three concentrations (5, 40 and 70 mg L(-1)). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/24206716/Rapid_tea_catechins_and_caffeine_determination_by_HPLC_using_microwave_assisted_extraction_and_silica_monolithic_column_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(13)01396-4 DB - PRIME DP - Unbound Medicine ER -