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Kinetics of eicosapentaenoic acid in brain, heart and liver of conscious rats fed a high n-3 PUFA containing diet.
Prostaglandins Leukot Essent Fatty Acids. 2013 Nov-Dec; 89(6):403-12.PL

Abstract

Eicosapentaenoic acid (EPA, 20:5n-3), a precursor of docosahexaenoic acid (DHA), may benefit cardiovascular and brain health. Quantifying EPA's in vivo kinetics might elucidate these effects. [1-(14)C]EPA was infused i.v. for 5min in unanesthetized male rats fed a standard EPA-DHA diet. Plasma and microwaved tissue were analyzed. Kinetic parameters were calculated using our compartmental model. At 5min, 31-48% of labeled EPA in brain and heart was oxidized, 7% in liver. EPA incorporation rates from brain and liver precursor EPA-CoA pools into lipids, mainly phospholipids, were 36 and 2529nmol/s/g×10(-4), insignificant for heart. Deacylation-reacylation half-lives were 22h and 38-128min. Conversion rates to DHA equaled 0.65 and 25.1nmol/s/g×10(-4), respectively. The low brain concentration and incorporation rate and high oxidation of EPA suggest that, if EPA has a beneficial effect in brain, it might result from its suppression of peripheral inflammation and hepatic conversion to bioactive DHA.

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Authors+Show Affiliations

Igarashi M
Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Building 9, Room 1S126, Bethesda, MD 20892, USA. Electronic address: miki.i@uci.edu.
Chang L
No affiliation info available
Ma K
No affiliation info available
Rapoport SI
No affiliation info available

MeSH

Acyl Coenzyme AAnimalsBrainBrain ChemistryCarbon RadioisotopesDocosahexaenoic AcidsEicosapentaenoic AcidFatty Acids, Omega-3Half-LifeKineticsLiverMaleMyocardiumOxidation-ReductionRatsRats, Inbred F344

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

24209500

Citation

Igarashi, Miki, et al. "Kinetics of Eicosapentaenoic Acid in Brain, Heart and Liver of Conscious Rats Fed a High N-3 PUFA Containing Diet." Prostaglandins, Leukotrienes, and Essential Fatty Acids, vol. 89, no. 6, 2013, pp. 403-12.
Igarashi M, Chang L, Ma K, et al. Kinetics of eicosapentaenoic acid in brain, heart and liver of conscious rats fed a high n-3 PUFA containing diet. Prostaglandins Leukot Essent Fatty Acids. 2013;89(6):403-12.
Igarashi, M., Chang, L., Ma, K., & Rapoport, S. I. (2013). Kinetics of eicosapentaenoic acid in brain, heart and liver of conscious rats fed a high n-3 PUFA containing diet. Prostaglandins, Leukotrienes, and Essential Fatty Acids, 89(6), 403-12. https://doi.org/10.1016/j.plefa.2013.09.004
Igarashi M, et al. Kinetics of Eicosapentaenoic Acid in Brain, Heart and Liver of Conscious Rats Fed a High N-3 PUFA Containing Diet. Prostaglandins Leukot Essent Fatty Acids. 2013 Nov-Dec;89(6):403-12. PubMed PMID: 24209500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetics of eicosapentaenoic acid in brain, heart and liver of conscious rats fed a high n-3 PUFA containing diet. AU - Igarashi,Miki, AU - Chang,Lisa, AU - Ma,Kaizong, AU - Rapoport,Stanley I, Y1 - 2013/09/16/ PY - 2013/07/19/received PY - 2013/09/07/revised PY - 2013/09/07/accepted PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2014/6/17/medline KW - ALA KW - ARA KW - Brain KW - DHA KW - DPA KW - EPA KW - Eicosapentaenoic acid KW - FAME KW - GC KW - HPLC KW - Kinetics KW - LA KW - Metabolism KW - PC KW - PUFA KW - Rat docosahexaenoic KW - TLC KW - Turnover KW - arachidonic acid KW - docosahexaenoic acid (22:6n-3) KW - docosapentaenoic acid (22:5n-3) KW - eicosapentaenoic acid (20:5n-3) KW - fatty acid methyl ester KW - gas chromatography KW - high performance liquid chromatography KW - linoleic acid (18:2n-6) KW - phosphatidylcholine KW - polyunsaturated fatty acid KW - thin layer chromatography KW - α-linolenic acid (18:3n-3) SP - 403 EP - 12 JF - Prostaglandins, leukotrienes, and essential fatty acids JO - Prostaglandins Leukot Essent Fatty Acids VL - 89 IS - 6 N2 - Eicosapentaenoic acid (EPA, 20:5n-3), a precursor of docosahexaenoic acid (DHA), may benefit cardiovascular and brain health. Quantifying EPA's in vivo kinetics might elucidate these effects. [1-(14)C]EPA was infused i.v. for 5min in unanesthetized male rats fed a standard EPA-DHA diet. Plasma and microwaved tissue were analyzed. Kinetic parameters were calculated using our compartmental model. At 5min, 31-48% of labeled EPA in brain and heart was oxidized, 7% in liver. EPA incorporation rates from brain and liver precursor EPA-CoA pools into lipids, mainly phospholipids, were 36 and 2529nmol/s/g×10(-4), insignificant for heart. Deacylation-reacylation half-lives were 22h and 38-128min. Conversion rates to DHA equaled 0.65 and 25.1nmol/s/g×10(-4), respectively. The low brain concentration and incorporation rate and high oxidation of EPA suggest that, if EPA has a beneficial effect in brain, it might result from its suppression of peripheral inflammation and hepatic conversion to bioactive DHA. SN - 1532-2823 UR - https://www.unboundmedicine.com/medline/citation/24209500/Kinetics_of_eicosapentaenoic_acid_in_brain_heart_and_liver_of_conscious_rats_fed_a_high_n_3_PUFA_containing_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0952-3278(13)00183-X DB - PRIME DP - Unbound Medicine ER -