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Enhanced antioxidant effect of caffeic acid phenethyl ester and Trolox in combination against radiation induced-oxidative stress.
Chem Biol Interact. 2014 Jan 25; 207:7-15.CB

Abstract

Combinations of antioxidants are believed to be more effective than single antioxidant because when antioxidants are combined they support each other synergistically to create a magnified effect. Discovering the enhancer effects or synergies between bioactive components is valuable for resisting oxidative stress and improving health benefits. The aim of this study was to investigate a possible cooperation of natural antioxidant caffeic acid phenethyl ester (CAPE) with synthetic antioxidant Trolox in the model systems of chemical generation of free radicals, lipid peroxidation of microsomes and radiation-induced oxidative injury in L929 cells. Based on the intermolecular interaction between CAPE and Trolox, the present study shows a synergistic effect of CAPE and Trolox in combination on elimination of three different free radicals and inhibition of lipid peroxidation initiated by three different systems. CAPE and Trolox added simultaneously to the L929 cells exerted an enhanced preventive effect on the oxidative injury induced by radiation through decreasing ROS generation, protecting plasma membrane and increasing the ratios of reduced glutathione/oxidized glutathione and the expression of key antioxidant enzymes mediated by nuclear factor erythroid 2 p45-related factor 2 (Nrf2). Our results showed for the first time that administration of CAPE and Trolox in combination may exert synergistic antioxidant effects, and further indicate that CAPE and Trolox combination functions mainly through scavenging ROS directly, inhibiting lipid peroxidation and promoting redox cycle of GSH mediated by Nrf2-regulated glutathione peroxidase and glutathione reductase expression.

Authors+Show Affiliations

Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.Department of Toxicology, Shaanxi Provincial Key Lab of Free Radical Biology and Medicine, The Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China. Electronic address: cx-hai@fmmu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24211618

Citation

Bai, Hua, et al. "Enhanced Antioxidant Effect of Caffeic Acid Phenethyl Ester and Trolox in Combination Against Radiation Induced-oxidative Stress." Chemico-biological Interactions, vol. 207, 2014, pp. 7-15.
Bai H, Liu R, Chen HL, et al. Enhanced antioxidant effect of caffeic acid phenethyl ester and Trolox in combination against radiation induced-oxidative stress. Chem Biol Interact. 2014;207:7-15.
Bai, H., Liu, R., Chen, H. L., Zhang, W., Wang, X., Zhang, X. D., Li, W. L., & Hai, C. X. (2014). Enhanced antioxidant effect of caffeic acid phenethyl ester and Trolox in combination against radiation induced-oxidative stress. Chemico-biological Interactions, 207, 7-15. https://doi.org/10.1016/j.cbi.2013.10.022
Bai H, et al. Enhanced Antioxidant Effect of Caffeic Acid Phenethyl Ester and Trolox in Combination Against Radiation Induced-oxidative Stress. Chem Biol Interact. 2014 Jan 25;207:7-15. PubMed PMID: 24211618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced antioxidant effect of caffeic acid phenethyl ester and Trolox in combination against radiation induced-oxidative stress. AU - Bai,Hua, AU - Liu,Rui, AU - Chen,Hong-Li, AU - Zhang,Wei, AU - Wang,Xin, AU - Zhang,Xiao-Di, AU - Li,Wen-Li, AU - Hai,Chun-Xu, Y1 - 2013/11/06/ PY - 2013/06/13/received PY - 2013/10/17/revised PY - 2013/10/28/accepted PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2014/2/26/medline KW - Caffeic acid phenethyl ester KW - Nrf2 KW - Oxidative stress KW - Radiation KW - Trolox SP - 7 EP - 15 JF - Chemico-biological interactions JO - Chem Biol Interact VL - 207 N2 - Combinations of antioxidants are believed to be more effective than single antioxidant because when antioxidants are combined they support each other synergistically to create a magnified effect. Discovering the enhancer effects or synergies between bioactive components is valuable for resisting oxidative stress and improving health benefits. The aim of this study was to investigate a possible cooperation of natural antioxidant caffeic acid phenethyl ester (CAPE) with synthetic antioxidant Trolox in the model systems of chemical generation of free radicals, lipid peroxidation of microsomes and radiation-induced oxidative injury in L929 cells. Based on the intermolecular interaction between CAPE and Trolox, the present study shows a synergistic effect of CAPE and Trolox in combination on elimination of three different free radicals and inhibition of lipid peroxidation initiated by three different systems. CAPE and Trolox added simultaneously to the L929 cells exerted an enhanced preventive effect on the oxidative injury induced by radiation through decreasing ROS generation, protecting plasma membrane and increasing the ratios of reduced glutathione/oxidized glutathione and the expression of key antioxidant enzymes mediated by nuclear factor erythroid 2 p45-related factor 2 (Nrf2). Our results showed for the first time that administration of CAPE and Trolox in combination may exert synergistic antioxidant effects, and further indicate that CAPE and Trolox combination functions mainly through scavenging ROS directly, inhibiting lipid peroxidation and promoting redox cycle of GSH mediated by Nrf2-regulated glutathione peroxidase and glutathione reductase expression. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/24211618/Enhanced_antioxidant_effect_of_caffeic_acid_phenethyl_ester_and_Trolox_in_combination_against_radiation_induced_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(13)00276-7 DB - PRIME DP - Unbound Medicine ER -