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Anti-inflammatory and antitumoural effects of Uncaria guianensis bark.
J Ethnopharmacol 2013; 150(3):1154-62JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Uncaria guianensis (Aublet) Gmell (Rubiaceae) is a medicinal plant from the jungles of South and Central America, used to treat cancer, arthritis, diabetes, and inflammation. Evaluate the anti-inflammatory and anti-tumor effects of Uncaria guianensis preparations.

MATERIALS AND METHODS

Bio-guided fractionation of a hydroethanolic extract of Uncaria guianensis was performed, evaluating the fractions and subfractions for their effect on inflammatory mediators, tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and prostaglandin E2 (PGE2) by ELISA and nitric oxide (NO) by the Griess reaction in cultured supernatant from RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). The expression of cyclooxygenase 2 (COX-2), inducible nitric oxide synthase (iNOS) and inhibitor of κB (IκB) were investigated in RAW 264.7 macrophages by flow cytometry. The activity of NF-κB in HeLa cells transfected with a luciferase reporter system was determined. The effect of Uncaria guianensis on the inflammatory response in vivo was assessed in BALB/c mice stimulated with LPS, on rat paw oedema induced by carrageenan, and on tumour growth and lung metastasis in BALB/c mice inoculated with 4T1 mammary tumour cells. Immune cell infiltrates and inflammatory mediators were evaluated in the tumour by immunohistochemistry.

RESULTS

Sub-fraction Ug AIV inhibited, to varying degrees, NO, TNF-α, IL-6 and PGE2 production by macrophages in vitro (30 μg/ml) and in the serum of LPS-challenged mice (5 mg/kg). Macrophage expression of Cox-2 was inhibited (35%), IκB degradation was completely inhibited and NF-κB activation was inhibited (70%) by Ug AIV at 30 μg/ml. Ug AIV decreased paw oedema by 86% (5 mg/kg) and serum NO and TNF-α by 45% and 65% respectively. Ug AIV reduced 4T1 mammary tumour growth by 91% on day 33 post-inoculation as well as the levels of serum NO, IL-6 and TNF-α in the same animals. Ug AIV decreased the number of tumour-infiltrating T lymphocytes, macrophages and neutrophils as well as the number of cells positive for COX-2, iNOS, IL-6, TNF-α and p65.

CONCLUSIONS

As Ug AIV was not cytotoxic for tumour cells or macrophages, its anti-tumour effect may be due to a reduction in pro-tumoural inflammatory processes in the tumour microenvironment, possibly mediated through NF-κB.

Authors+Show Affiliations

Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado 20632, Caracas 1020-A, Venezuela.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24212077

Citation

Urdanibia, I, et al. "Anti-inflammatory and Antitumoural Effects of Uncaria Guianensis Bark." Journal of Ethnopharmacology, vol. 150, no. 3, 2013, pp. 1154-62.
Urdanibia I, Michelangeli F, Ruiz MC, et al. Anti-inflammatory and antitumoural effects of Uncaria guianensis bark. J Ethnopharmacol. 2013;150(3):1154-62.
Urdanibia, I., Michelangeli, F., Ruiz, M. C., Milano, B., & Taylor, P. (2013). Anti-inflammatory and antitumoural effects of Uncaria guianensis bark. Journal of Ethnopharmacology, 150(3), pp. 1154-62. doi:10.1016/j.jep.2013.10.055.
Urdanibia I, et al. Anti-inflammatory and Antitumoural Effects of Uncaria Guianensis Bark. J Ethnopharmacol. 2013 Dec 12;150(3):1154-62. PubMed PMID: 24212077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory and antitumoural effects of Uncaria guianensis bark. AU - Urdanibia,I, AU - Michelangeli,F, AU - Ruiz,M-C, AU - Milano,B, AU - Taylor,P, Y1 - 2013/11/07/ PY - 2013/08/15/received PY - 2013/09/16/revised PY - 2013/10/23/accepted PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2014/8/26/medline KW - COX-2 KW - IL KW - Il-6 KW - IκB KW - LPS KW - NF-κB KW - NO KW - PGE(2) KW - TNF-α KW - cyclooxygenase 2 KW - iNOS KW - inducible nitric oxide synthase KW - inhibitor of κB KW - interleukin KW - lipopolysaccharide KW - nitric oxide KW - prostaglandin E(2) KW - tumour necrosis factor alpha SP - 1154 EP - 62 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 150 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Uncaria guianensis (Aublet) Gmell (Rubiaceae) is a medicinal plant from the jungles of South and Central America, used to treat cancer, arthritis, diabetes, and inflammation. Evaluate the anti-inflammatory and anti-tumor effects of Uncaria guianensis preparations. MATERIALS AND METHODS: Bio-guided fractionation of a hydroethanolic extract of Uncaria guianensis was performed, evaluating the fractions and subfractions for their effect on inflammatory mediators, tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and prostaglandin E2 (PGE2) by ELISA and nitric oxide (NO) by the Griess reaction in cultured supernatant from RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). The expression of cyclooxygenase 2 (COX-2), inducible nitric oxide synthase (iNOS) and inhibitor of κB (IκB) were investigated in RAW 264.7 macrophages by flow cytometry. The activity of NF-κB in HeLa cells transfected with a luciferase reporter system was determined. The effect of Uncaria guianensis on the inflammatory response in vivo was assessed in BALB/c mice stimulated with LPS, on rat paw oedema induced by carrageenan, and on tumour growth and lung metastasis in BALB/c mice inoculated with 4T1 mammary tumour cells. Immune cell infiltrates and inflammatory mediators were evaluated in the tumour by immunohistochemistry. RESULTS: Sub-fraction Ug AIV inhibited, to varying degrees, NO, TNF-α, IL-6 and PGE2 production by macrophages in vitro (30 μg/ml) and in the serum of LPS-challenged mice (5 mg/kg). Macrophage expression of Cox-2 was inhibited (35%), IκB degradation was completely inhibited and NF-κB activation was inhibited (70%) by Ug AIV at 30 μg/ml. Ug AIV decreased paw oedema by 86% (5 mg/kg) and serum NO and TNF-α by 45% and 65% respectively. Ug AIV reduced 4T1 mammary tumour growth by 91% on day 33 post-inoculation as well as the levels of serum NO, IL-6 and TNF-α in the same animals. Ug AIV decreased the number of tumour-infiltrating T lymphocytes, macrophages and neutrophils as well as the number of cells positive for COX-2, iNOS, IL-6, TNF-α and p65. CONCLUSIONS: As Ug AIV was not cytotoxic for tumour cells or macrophages, its anti-tumour effect may be due to a reduction in pro-tumoural inflammatory processes in the tumour microenvironment, possibly mediated through NF-κB. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24212077/Anti_inflammatory_and_antitumoural_effects_of_Uncaria_guianensis_bark_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00780-0 DB - PRIME DP - Unbound Medicine ER -