Tags

Type your tag names separated by a space and hit enter

Selenium attenuates high glucose-induced ROS/TLR-4 involved apoptosis of rat cardiomyocyte.
Biol Trace Elem Res. 2013 Dec; 156(1-3):262-70.BT

Abstract

The potential mechanism of high glucose-induced cardiomyocyte apoptosis and selenium's protective effects were investigated in this study. Myocytes isolated from neonate rats were cultured in high-glucose medium (25.5 mmol/L glucose) to mimic sustained hyperglycemia. Before high-glucose incubation, myocytes were pretreated by sodium selenite solution. Cell apoptosis was evaluated by annexin V/propidium iodide (PI) staining and caspase activation. Expression of Toll-like receptor 4 (TLR-4) and myeloid differentiation factor 88 (MyD-88) was examined at both mRNA and protein levels. The intracellular reactive oxygen species (ROS) production and glutathione peroxidase (GPx) activity in myocytes were also detected. We found high glucose-induced cell apoptosis and activation of TLR-4/MyD-88/caspase-8/caspase-3 signaling, accompanied by increased production of ROS. Selenium pretreatment attenuated apoptosis in high glucose-incubated myocytes, and mechanically, this protective effect was found to be associated with attenuating oxidative status by increasing activity of GPx, decreasing the generation of ROS, as well as inhibition of the activation of TLR-4/MyD-88/caspase-8/caspase-3 signaling in myocytes. These results suggest that activation of TLR-4/MyD-88 signaling pathway plays an important role in high glucose-induced cardiomyocyte apoptosis. Additionally, by modulating TLR-4/MyD-88 signaling pathway, which is linked to ROS formation, selenium exerts its antioxidative and antiapoptotic effects in high glucose-incubated myocytes.

Authors+Show Affiliations

Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24214856

Citation

Liu, Zhong-Wei, et al. "Selenium Attenuates High Glucose-induced ROS/TLR-4 Involved Apoptosis of Rat Cardiomyocyte." Biological Trace Element Research, vol. 156, no. 1-3, 2013, pp. 262-70.
Liu ZW, Zhu HT, Chen KL, et al. Selenium attenuates high glucose-induced ROS/TLR-4 involved apoptosis of rat cardiomyocyte. Biol Trace Elem Res. 2013;156(1-3):262-70.
Liu, Z. W., Zhu, H. T., Chen, K. L., Qiu, C., Tang, K. F., & Niu, X. L. (2013). Selenium attenuates high glucose-induced ROS/TLR-4 involved apoptosis of rat cardiomyocyte. Biological Trace Element Research, 156(1-3), 262-70. https://doi.org/10.1007/s12011-013-9857-7
Liu ZW, et al. Selenium Attenuates High Glucose-induced ROS/TLR-4 Involved Apoptosis of Rat Cardiomyocyte. Biol Trace Elem Res. 2013;156(1-3):262-70. PubMed PMID: 24214856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium attenuates high glucose-induced ROS/TLR-4 involved apoptosis of rat cardiomyocyte. AU - Liu,Zhong-Wei, AU - Zhu,Hai-Tao, AU - Chen,Kun-Lun, AU - Qiu,Chuan, AU - Tang,Kai-Fa, AU - Niu,Xiao-Lin, Y1 - 2013/11/10/ PY - 2013/08/30/received PY - 2013/10/30/accepted PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2014/7/22/medline SP - 262 EP - 70 JF - Biological trace element research JO - Biol Trace Elem Res VL - 156 IS - 1-3 N2 - The potential mechanism of high glucose-induced cardiomyocyte apoptosis and selenium's protective effects were investigated in this study. Myocytes isolated from neonate rats were cultured in high-glucose medium (25.5 mmol/L glucose) to mimic sustained hyperglycemia. Before high-glucose incubation, myocytes were pretreated by sodium selenite solution. Cell apoptosis was evaluated by annexin V/propidium iodide (PI) staining and caspase activation. Expression of Toll-like receptor 4 (TLR-4) and myeloid differentiation factor 88 (MyD-88) was examined at both mRNA and protein levels. The intracellular reactive oxygen species (ROS) production and glutathione peroxidase (GPx) activity in myocytes were also detected. We found high glucose-induced cell apoptosis and activation of TLR-4/MyD-88/caspase-8/caspase-3 signaling, accompanied by increased production of ROS. Selenium pretreatment attenuated apoptosis in high glucose-incubated myocytes, and mechanically, this protective effect was found to be associated with attenuating oxidative status by increasing activity of GPx, decreasing the generation of ROS, as well as inhibition of the activation of TLR-4/MyD-88/caspase-8/caspase-3 signaling in myocytes. These results suggest that activation of TLR-4/MyD-88 signaling pathway plays an important role in high glucose-induced cardiomyocyte apoptosis. Additionally, by modulating TLR-4/MyD-88 signaling pathway, which is linked to ROS formation, selenium exerts its antioxidative and antiapoptotic effects in high glucose-incubated myocytes. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/24214856/Selenium_attenuates_high_glucose_induced_ROS/TLR_4_involved_apoptosis_of_rat_cardiomyocyte_ L2 - https://dx.doi.org/10.1007/s12011-013-9857-7 DB - PRIME DP - Unbound Medicine ER -