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Protective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver.
Toxicol Ind Health 2016; 32(4):694-706TI

Abstract

This study aimed to investigate the antioxidant properties of different fractions obtained from the fruits of Lawsonia inermis, a widely used medicinal plant, against carbon tetrachloride (CCl4)-induced oxidative stress in rat liver. The results show that several fractions obtained from L. inermis fruits possessed important antioxidant activity. Among them, the ethyl acetate (EA) fraction showed the highest antioxidant activity. Then, EA fraction was selected for the purification of potential antioxidant compounds. The hepatoprotective effects of EA fraction and its most active constituent, gallic acid (GA), were evaluated against CCl4-induced hepatotoxicity in rats. CCl4 induced oxidative stress by a significant rise in serum marker enzymes. However, pretreatment of rats with EA fraction of fruits of L. inermis at a dose of 250 mg kg(-1)body weight and GA significantly lowered some serum biochemical parameters (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase) in treated rats. A significant reduction in hepatic thiobarbituric acid reactive substances and an increase in antioxidant enzymes namely superoxide dismutase, catalase, and glutathione peroxidase by treatment with plant extract and GA, against CCl4-treated rats, were observed. Histopathological examinations showed extensive liver injuries, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration. This potential antioxidant activity is comparable to those of the major purified antioxidant compound, GA. Based on these results, it was observed that fruits of L. inermis protect liver from oxidative stress induced by CCl4 and thus help in evaluation of traditional claim on this plant.

Authors+Show Affiliations

Biopesticides Team (LPIP), Center of Biotechnology of Sfax, Sfax, Tunisia.Laboratory of Animal Ecophysiology, Faculty of Science, University of Sfax, Sfax, Tunisia mongifss@yahoo.fr.Laboratoire MAPIEM (EA 4323), Equipe Biofouling and Substances Naturelles Marines, Université du Sud Toulon-Var, Toulon, France.Laboratoire MAPIEM (EA 4323), Equipe Biofouling and Substances Naturelles Marines, Université du Sud Toulon-Var, Toulon, France.Laboratory of Pharmacology, School of Medicine, University of Sfax, Sfax, Tunisia.Biochemistry Laboratory, CHU Hedi Chaker of Sfax, Sfax, Tunisia.Laboratory of Animal Ecophysiology, Faculty of Science, University of Sfax, Sfax, Tunisia.Biopesticides Team (LPIP), Center of Biotechnology of Sfax, Sfax, Tunisia College of Arts and Sciences, University of Qatar, Doha, Qatar.Biopesticides Team (LPIP), Center of Biotechnology of Sfax, Sfax, Tunisia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24215067

Citation

Hsouna, Anis Ben, et al. "Protective Effects of Ethyl Acetate Fraction of Lawsonia Inermis Fruits Extract Against Carbon Tetrachloride-induced Oxidative Damage in Rat Liver." Toxicology and Industrial Health, vol. 32, no. 4, 2016, pp. 694-706.
Hsouna AB, Mongi S, Culioli G, et al. Protective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver. Toxicol Ind Health. 2016;32(4):694-706.
Hsouna, A. B., Mongi, S., Culioli, G., Blache, Y., Ghlissi, Z., Chaabane, R., ... Trigui, M. (2016). Protective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver. Toxicology and Industrial Health, 32(4), pp. 694-706. doi:10.1177/0748233713502839.
Hsouna AB, et al. Protective Effects of Ethyl Acetate Fraction of Lawsonia Inermis Fruits Extract Against Carbon Tetrachloride-induced Oxidative Damage in Rat Liver. Toxicol Ind Health. 2016;32(4):694-706. PubMed PMID: 24215067.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of ethyl acetate fraction of Lawsonia inermis fruits extract against carbon tetrachloride-induced oxidative damage in rat liver. AU - Hsouna,Anis Ben, AU - Mongi,Saoudi, AU - Culioli,Gérald, AU - Blache,Yves, AU - Ghlissi,Zohra, AU - Chaabane,Rim, AU - El Feki,Abdelfattah, AU - Jaoua,Samir, AU - Trigui,Mohamed, Y1 - 2013/11/08/ PY - 2013/11/12/entrez PY - 2013/11/12/pubmed PY - 2016/12/29/medline KW - Lawsonia inermis KW - beneficial effect KW - carbon tetrachloride KW - hepatotoxicity KW - oxidative stress SP - 694 EP - 706 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 32 IS - 4 N2 - This study aimed to investigate the antioxidant properties of different fractions obtained from the fruits of Lawsonia inermis, a widely used medicinal plant, against carbon tetrachloride (CCl4)-induced oxidative stress in rat liver. The results show that several fractions obtained from L. inermis fruits possessed important antioxidant activity. Among them, the ethyl acetate (EA) fraction showed the highest antioxidant activity. Then, EA fraction was selected for the purification of potential antioxidant compounds. The hepatoprotective effects of EA fraction and its most active constituent, gallic acid (GA), were evaluated against CCl4-induced hepatotoxicity in rats. CCl4 induced oxidative stress by a significant rise in serum marker enzymes. However, pretreatment of rats with EA fraction of fruits of L. inermis at a dose of 250 mg kg(-1)body weight and GA significantly lowered some serum biochemical parameters (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase) in treated rats. A significant reduction in hepatic thiobarbituric acid reactive substances and an increase in antioxidant enzymes namely superoxide dismutase, catalase, and glutathione peroxidase by treatment with plant extract and GA, against CCl4-treated rats, were observed. Histopathological examinations showed extensive liver injuries, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration. This potential antioxidant activity is comparable to those of the major purified antioxidant compound, GA. Based on these results, it was observed that fruits of L. inermis protect liver from oxidative stress induced by CCl4 and thus help in evaluation of traditional claim on this plant. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/24215067/Protective_effects_of_ethyl_acetate_fraction_of_Lawsonia_inermis_fruits_extract_against_carbon_tetrachloride_induced_oxidative_damage_in_rat_liver_ L2 - http://journals.sagepub.com/doi/full/10.1177/0748233713502839?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -