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Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content.
Clin Sci (Lond). 2014 Apr; 126(8):557-66.CS

Abstract

Muscle disuse leads to a considerable loss in skeletal muscle mass and strength. However, the cellular mechanisms underlying disuse-induced muscle fibre atrophy remain to be elucidated. Therefore we assessed the effect of muscle disuse on the CSA (cross-sectional area), muscle fibre size, satellite cell content and associated myocellular signalling pathways of the quadriceps muscle. A total of 12 healthy young (24±1 years of age) men were subjected to 2 weeks of one-legged knee immobilization via a full-leg cast. Before and immediately after the immobilization period and after 6 weeks of natural rehabilitation, muscle strength [1RM (one-repetition maximum)], muscle CSA [single slice CT (computed tomography) scan] and muscle fibre type characteristics (muscle biopsies) were assessed. Protein and/or mRNA expression of key genes [i.e. MYOD (myogenic differentiation), MYOG (myogenin) and MSTN (myostatin)] in the satellite cell regulatory pathways were determined using Western blotting and RT-PCR (real-time PCR) analyses respectively. The present study found that quadriceps CSA declined following immobilization by 8±2% (P<0.05). In agreement, both type I and type II muscle fibre size decreased 7±3% and 13±4% respectively (P<0.05). No changes were observed in satellite cell content following immobilization in either type I or type II muscle fibres. Muscle MYOG mRNA expression doubled (P<0.05), whereas MSTN protein expression decreased 30±9% (P<0.05) following immobilization. Muscle mass and strength returned to the baseline values within 6 weeks of recovery without any specific rehabilitative programme. In conclusion, 2 weeks of muscle disuse leads to considerable loss in skeletal muscle mass and strength. The loss in muscle mass was attributed to both type I and type II muscle fibre atrophy, and was not accompanied by a decline in satellite cell content.

Authors+Show Affiliations

*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.†Departments of Epidemiology and Surgery, School for Public Health and Primary Care (CAPHRI), Maastricht University Medical Centre+, Maastricht, The Netherlands.‡Department of Surgery, Maastricht University Medical Centre+, Maastricht, The Netherlands.§Department of Neuroscience, School of Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24215591

Citation

Snijders, Tim, et al. "Muscle Disuse Atrophy Is Not Accompanied By Changes in Skeletal Muscle Satellite Cell Content." Clinical Science (London, England : 1979), vol. 126, no. 8, 2014, pp. 557-66.
Snijders T, Wall BT, Dirks ML, et al. Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content. Clin Sci (Lond). 2014;126(8):557-66.
Snijders, T., Wall, B. T., Dirks, M. L., Senden, J. M., Hartgens, F., Dolmans, J., Losen, M., Verdijk, L. B., & van Loon, L. J. (2014). Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content. Clinical Science (London, England : 1979), 126(8), 557-66. https://doi.org/10.1042/CS20130295
Snijders T, et al. Muscle Disuse Atrophy Is Not Accompanied By Changes in Skeletal Muscle Satellite Cell Content. Clin Sci (Lond). 2014;126(8):557-66. PubMed PMID: 24215591.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content. AU - Snijders,Tim, AU - Wall,Benjamin T, AU - Dirks,Marlou L, AU - Senden,Joan M G, AU - Hartgens,Fred, AU - Dolmans,John, AU - Losen,Mario, AU - Verdijk,Lex B, AU - van Loon,Luc J C, PY - 2013/11/13/entrez PY - 2013/11/13/pubmed PY - 2014/3/4/medline SP - 557 EP - 66 JF - Clinical science (London, England : 1979) JO - Clin Sci (Lond) VL - 126 IS - 8 N2 - Muscle disuse leads to a considerable loss in skeletal muscle mass and strength. However, the cellular mechanisms underlying disuse-induced muscle fibre atrophy remain to be elucidated. Therefore we assessed the effect of muscle disuse on the CSA (cross-sectional area), muscle fibre size, satellite cell content and associated myocellular signalling pathways of the quadriceps muscle. A total of 12 healthy young (24±1 years of age) men were subjected to 2 weeks of one-legged knee immobilization via a full-leg cast. Before and immediately after the immobilization period and after 6 weeks of natural rehabilitation, muscle strength [1RM (one-repetition maximum)], muscle CSA [single slice CT (computed tomography) scan] and muscle fibre type characteristics (muscle biopsies) were assessed. Protein and/or mRNA expression of key genes [i.e. MYOD (myogenic differentiation), MYOG (myogenin) and MSTN (myostatin)] in the satellite cell regulatory pathways were determined using Western blotting and RT-PCR (real-time PCR) analyses respectively. The present study found that quadriceps CSA declined following immobilization by 8±2% (P<0.05). In agreement, both type I and type II muscle fibre size decreased 7±3% and 13±4% respectively (P<0.05). No changes were observed in satellite cell content following immobilization in either type I or type II muscle fibres. Muscle MYOG mRNA expression doubled (P<0.05), whereas MSTN protein expression decreased 30±9% (P<0.05) following immobilization. Muscle mass and strength returned to the baseline values within 6 weeks of recovery without any specific rehabilitative programme. In conclusion, 2 weeks of muscle disuse leads to considerable loss in skeletal muscle mass and strength. The loss in muscle mass was attributed to both type I and type II muscle fibre atrophy, and was not accompanied by a decline in satellite cell content. SN - 1470-8736 UR - https://www.unboundmedicine.com/medline/citation/24215591/Muscle_disuse_atrophy_is_not_accompanied_by_changes_in_skeletal_muscle_satellite_cell_content_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/CS20130295 DB - PRIME DP - Unbound Medicine ER -