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[Effect of shuganjianpifang on the expression of BCL-2 and BAX in rats livers with hepatic fibrosis].
Zhong Yao Cai. 2013 May; 36(5):776-80.ZY

Abstract

OBJECTIVE

To observe the effect of shuganjianpifang on BCL-2, BAX expressions in livers of hepatic fibrosis rats and its mechanism.

METHODS

Sixty SD rats were randomly divided into six groups. Rat liver fibrosis was induced by CCl4 twice a week for 12 weeks. Shuganjianpifang was used daily via lavage at 7th week for 6 weeks. The contents of ALT, AST, T-BiL and Alb in serum were measured. Liver samples were taken to examine the degree of liver fibrosis by HE staining. The experessions of BCL-2 and BAX were detected by immunohisto chemistry. The expression of BCL-2, BAX mRNA was detected by RT-PCR technology.

RESULTS

As compared with the fibrotic model group, shuganjianpifang significantly reduced histopathological change, such as steatosis, deposition, decreased the contents of ALT, AST and T-BiL, up-regulated the expression of Alb. Meanwhile shuganjianpifang could effectively inhibit the expression of BAX, significantly enhanced the expression BCL-2 in liver fibrosis rats.

CONCLUSION

Shuganjianpifang can resist hepatic fibrosis possibly by up-regulating BCL-2 expression and down-regulating BAX expression.

Authors+Show Affiliations

The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of TCM, Hefei 230031, China. jhanbing@163.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

24218972

Citation

Jiang, Hui, et al. "[Effect of Shuganjianpifang On the Expression of BCL-2 and BAX in Rats Livers With Hepatic Fibrosis]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 36, no. 5, 2013, pp. 776-80.
Jiang H, Gao JR, Chen JF, et al. [Effect of shuganjianpifang on the expression of BCL-2 and BAX in rats livers with hepatic fibrosis]. Zhong Yao Cai. 2013;36(5):776-80.
Jiang, H., Gao, J. R., Chen, J. F., & Ji, W. B. (2013). [Effect of shuganjianpifang on the expression of BCL-2 and BAX in rats livers with hepatic fibrosis]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 36(5), 776-80.
Jiang H, et al. [Effect of Shuganjianpifang On the Expression of BCL-2 and BAX in Rats Livers With Hepatic Fibrosis]. Zhong Yao Cai. 2013;36(5):776-80. PubMed PMID: 24218972.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of shuganjianpifang on the expression of BCL-2 and BAX in rats livers with hepatic fibrosis]. AU - Jiang,Hui, AU - Gao,Jia-Rong, AU - Chen,Jin-Feng, AU - Ji,Wen-Bo, PY - 2013/11/14/entrez PY - 2013/11/14/pubmed PY - 2014/1/24/medline SP - 776 EP - 80 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 36 IS - 5 N2 - OBJECTIVE: To observe the effect of shuganjianpifang on BCL-2, BAX expressions in livers of hepatic fibrosis rats and its mechanism. METHODS: Sixty SD rats were randomly divided into six groups. Rat liver fibrosis was induced by CCl4 twice a week for 12 weeks. Shuganjianpifang was used daily via lavage at 7th week for 6 weeks. The contents of ALT, AST, T-BiL and Alb in serum were measured. Liver samples were taken to examine the degree of liver fibrosis by HE staining. The experessions of BCL-2 and BAX were detected by immunohisto chemistry. The expression of BCL-2, BAX mRNA was detected by RT-PCR technology. RESULTS: As compared with the fibrotic model group, shuganjianpifang significantly reduced histopathological change, such as steatosis, deposition, decreased the contents of ALT, AST and T-BiL, up-regulated the expression of Alb. Meanwhile shuganjianpifang could effectively inhibit the expression of BAX, significantly enhanced the expression BCL-2 in liver fibrosis rats. CONCLUSION: Shuganjianpifang can resist hepatic fibrosis possibly by up-regulating BCL-2 expression and down-regulating BAX expression. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/24218972/[Effect_of_shuganjianpifang_on_the_expression_of_BCL_2_and_BAX_in_rats_livers_with_hepatic_fibrosis]_ DB - PRIME DP - Unbound Medicine ER -