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Differential satellite cell density of type I and II fibres with lifelong endurance running in old men.
Acta Physiol (Oxf). 2014 Mar; 210(3):612-27.AP

Abstract

AIM

To investigate the influence of lifelong endurance running on the satellite cell pool of type I and type II fibres in healthy human skeletal muscle.

METHODS

Muscle biopsies were collected from 15 healthy old trained men (O-Tr) who had been running 43 ± 16 (mean ± SD) kilometres a week for 28 ± 9 years. Twelve age-matched untrained men (O-Un) and a group of young trained and young untrained men were recruited for comparison. Frozen sections were immunohistochemically stained for Pax7, type I myosin and laminin, from which fibre area, the number of satellite cells, and the relationship between these variables were determined.

RESULTS

In O-Un and O-Tr, type II fibres were smaller and contained fewer satellite cells than type I fibres. However, when expressed relative to fibre area, the difference in satellite cell content between fibre types was eliminated in O-Tr, but not O-Un. A strong positive relationship between fibre size and satellite cell content was detected in trained individuals. In line with a history of myofibre repair, a greater number of fibres with centrally located myonuclei were detected in O-Tr.

CONCLUSION

Lifelong endurance training (i) does not deplete the satellite cell pool and (ii) is associated with a similar density of satellite cells in type I and II fibres despite a failure to preserve the equal fibre type distribution of satellite cells observed in young individuals. Taken together, these data reveal a differential regulation of satellite cell content between fibre types, in young and old healthy men with dramatically different training histories.

Authors+Show Affiliations

Department of Orthopaedic Surgery M, Institute of Sports Medicine Copenhagen, Bispebjerg Hospital, Copenhagen, Denmark; Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24219628

Citation

Mackey, A L., et al. "Differential Satellite Cell Density of Type I and II Fibres With Lifelong Endurance Running in Old Men." Acta Physiologica (Oxford, England), vol. 210, no. 3, 2014, pp. 612-27.
Mackey AL, Karlsen A, Couppé C, et al. Differential satellite cell density of type I and II fibres with lifelong endurance running in old men. Acta Physiol (Oxf). 2014;210(3):612-27.
Mackey, A. L., Karlsen, A., Couppé, C., Mikkelsen, U. R., Nielsen, R. H., Magnusson, S. P., & Kjaer, M. (2014). Differential satellite cell density of type I and II fibres with lifelong endurance running in old men. Acta Physiologica (Oxford, England), 210(3), 612-27. https://doi.org/10.1111/apha.12195
Mackey AL, et al. Differential Satellite Cell Density of Type I and II Fibres With Lifelong Endurance Running in Old Men. Acta Physiol (Oxf). 2014;210(3):612-27. PubMed PMID: 24219628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential satellite cell density of type I and II fibres with lifelong endurance running in old men. AU - Mackey,A L, AU - Karlsen,A, AU - Couppé,C, AU - Mikkelsen,U R, AU - Nielsen,R H, AU - Magnusson,S P, AU - Kjaer,M, Y1 - 2013/12/12/ PY - 2013/08/02/received PY - 2013/09/23/revised PY - 2013/10/20/revised PY - 2013/11/08/accepted PY - 2013/11/14/entrez PY - 2013/11/14/pubmed PY - 2014/10/25/medline KW - Pax7 KW - ageing muscle KW - central nuclei KW - master athlete KW - satellite cell density SP - 612 EP - 27 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 210 IS - 3 N2 - AIM: To investigate the influence of lifelong endurance running on the satellite cell pool of type I and type II fibres in healthy human skeletal muscle. METHODS: Muscle biopsies were collected from 15 healthy old trained men (O-Tr) who had been running 43 ± 16 (mean ± SD) kilometres a week for 28 ± 9 years. Twelve age-matched untrained men (O-Un) and a group of young trained and young untrained men were recruited for comparison. Frozen sections were immunohistochemically stained for Pax7, type I myosin and laminin, from which fibre area, the number of satellite cells, and the relationship between these variables were determined. RESULTS: In O-Un and O-Tr, type II fibres were smaller and contained fewer satellite cells than type I fibres. However, when expressed relative to fibre area, the difference in satellite cell content between fibre types was eliminated in O-Tr, but not O-Un. A strong positive relationship between fibre size and satellite cell content was detected in trained individuals. In line with a history of myofibre repair, a greater number of fibres with centrally located myonuclei were detected in O-Tr. CONCLUSION: Lifelong endurance training (i) does not deplete the satellite cell pool and (ii) is associated with a similar density of satellite cells in type I and II fibres despite a failure to preserve the equal fibre type distribution of satellite cells observed in young individuals. Taken together, these data reveal a differential regulation of satellite cell content between fibre types, in young and old healthy men with dramatically different training histories. SN - 1748-1716 UR - https://www.unboundmedicine.com/medline/citation/24219628/Differential_satellite_cell_density_of_type_I_and_II_fibres_with_lifelong_endurance_running_in_old_men_ L2 - https://doi.org/10.1111/apha.12195 DB - PRIME DP - Unbound Medicine ER -