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T cell-depleted splenocytes from mice pre-immunized with neuroantigen in incomplete Freund's adjuvant involved in protection from experimental autoimmune encephalomyelitis.
Immunol Lett. 2014 Jan-Feb; 157(1-2):38-44.IL

Abstract

Mice immunized with neuroantigens in incomplete Freund's adjuvant (IFA) are resistant to subsequent induction of experimental autoimmune encephalomyelitis (EAE). The mechanisms involved in this protection are complex. Studies on relevant CD4(+) or CD8(+) T cells, including effective and regulatory T cells, have been performed by others. In this work, the effects of CD4(-)-, CD8(-)- splenocytes on protection from EAE in C57BL/6 mice which were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG)35-55 in IFA were evaluated. We observed that MOG-reactive CD4(+) T cells failed to be activated and proliferate when CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice were regarded as antigen-presenting cells (APC). It was shown that these APC expressed lower levels of major histocompatibility complex class II (MHC-II), CD80, and CD86 than naïve cells. In addition, CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice showed significantly higher levels of IL-10 mRNA expression. When the immunized-mice were induced to develop EAE, these cells secreted significantly higher levels of IL-10 and produced lower levels of IL-6, leading to decreased secretion of IL-17 and IFN-γ from MOG-specific CD4(+) T cells. The transfer of CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice was able to ameliorate the subsequent induction of EAE in recipient mice. Thus, MOG/IFA immunization can modulate CD4(-)-, CD8(-)- splenocytes by reducing the expression of antigen-presenting molecules and altering the levels of secreted cytokines. Our study reveals an additional mechanism involved in the protective effects of MOG/IFA pre-immunization in an EAE model.

Authors+Show Affiliations

Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China; Department of Clinical Laboratory, Shanghai Cancer Center, Fudan University, Shanghai, China.Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China; Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, USA. Electronic address: hong_jiang24@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24220208

Citation

Zheng, Hui, et al. "T Cell-depleted Splenocytes From Mice Pre-immunized With Neuroantigen in Incomplete Freund's Adjuvant Involved in Protection From Experimental Autoimmune Encephalomyelitis." Immunology Letters, vol. 157, no. 1-2, 2014, pp. 38-44.
Zheng H, Zhang H, Liu F, et al. T cell-depleted splenocytes from mice pre-immunized with neuroantigen in incomplete Freund's adjuvant involved in protection from experimental autoimmune encephalomyelitis. Immunol Lett. 2014;157(1-2):38-44.
Zheng, H., Zhang, H., Liu, F., Qi, Y., & Jiang, H. (2014). T cell-depleted splenocytes from mice pre-immunized with neuroantigen in incomplete Freund's adjuvant involved in protection from experimental autoimmune encephalomyelitis. Immunology Letters, 157(1-2), 38-44. https://doi.org/10.1016/j.imlet.2013.11.001
Zheng H, et al. T Cell-depleted Splenocytes From Mice Pre-immunized With Neuroantigen in Incomplete Freund's Adjuvant Involved in Protection From Experimental Autoimmune Encephalomyelitis. Immunol Lett. 2014 Jan-Feb;157(1-2):38-44. PubMed PMID: 24220208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - T cell-depleted splenocytes from mice pre-immunized with neuroantigen in incomplete Freund's adjuvant involved in protection from experimental autoimmune encephalomyelitis. AU - Zheng,Hui, AU - Zhang,Han, AU - Liu,Feng, AU - Qi,Yuanyuan, AU - Jiang,Hong, Y1 - 2013/11/09/ PY - 2013/08/21/received PY - 2013/11/01/accepted PY - 2013/11/14/entrez PY - 2013/11/14/pubmed PY - 2014/8/1/medline KW - APC KW - Antigen-presenting cell KW - CFA KW - CNS KW - EAE KW - Experimental autoimmune encephalomyelitis KW - IFA KW - Incomplete Freund's adjuvant KW - LPS KW - MFI KW - MHC-II KW - MOG KW - MS KW - PBS KW - SEM KW - antigen-presenting cells KW - central nervous system KW - complete Freunds adjuvant KW - counts per minute KW - cpm KW - experimental autoimmune encephalomyelitis KW - incomplete Freund's adjuvant KW - lipopolysaccharide KW - major histocompatibility complex class II KW - mean fluorescence intensity KW - multiple sclerosis KW - myelin oligodendrocyte glycoprotein KW - phosphate buffered saline KW - standard error of mean SP - 38 EP - 44 JF - Immunology letters JO - Immunol. Lett. VL - 157 IS - 1-2 N2 - Mice immunized with neuroantigens in incomplete Freund's adjuvant (IFA) are resistant to subsequent induction of experimental autoimmune encephalomyelitis (EAE). The mechanisms involved in this protection are complex. Studies on relevant CD4(+) or CD8(+) T cells, including effective and regulatory T cells, have been performed by others. In this work, the effects of CD4(-)-, CD8(-)- splenocytes on protection from EAE in C57BL/6 mice which were immunized with myelin oligodendrocyte glycoprotein 35-55 (MOG)35-55 in IFA were evaluated. We observed that MOG-reactive CD4(+) T cells failed to be activated and proliferate when CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice were regarded as antigen-presenting cells (APC). It was shown that these APC expressed lower levels of major histocompatibility complex class II (MHC-II), CD80, and CD86 than naïve cells. In addition, CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice showed significantly higher levels of IL-10 mRNA expression. When the immunized-mice were induced to develop EAE, these cells secreted significantly higher levels of IL-10 and produced lower levels of IL-6, leading to decreased secretion of IL-17 and IFN-γ from MOG-specific CD4(+) T cells. The transfer of CD4(-)-, CD8(-)- splenocytes from MOG/IFA-immunized mice was able to ameliorate the subsequent induction of EAE in recipient mice. Thus, MOG/IFA immunization can modulate CD4(-)-, CD8(-)- splenocytes by reducing the expression of antigen-presenting molecules and altering the levels of secreted cytokines. Our study reveals an additional mechanism involved in the protective effects of MOG/IFA pre-immunization in an EAE model. SN - 1879-0542 UR - https://www.unboundmedicine.com/medline/citation/24220208/T_cell_depleted_splenocytes_from_mice_pre_immunized_with_neuroantigen_in_incomplete_Freund's_adjuvant_involved_in_protection_from_experimental_autoimmune_encephalomyelitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2478(13)00168-5 DB - PRIME DP - Unbound Medicine ER -