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Activation of early phase of adipogenesis through Krüppel-like factor KLF9-mediated, enhanced expression of CCAAT/enhancer-binding protein β in 3T3-L1 cells.
Gene. 2014 Jan 25; 534(2):169-76.GENE

Abstract

In this study, we found that Krüppel-like factor (KLF) 9 activate the progression of the early phase of adipocyte differentiation in mouse adipocytic 3T3-L1 cells. KLF9 mRNA was detected in preadipocytes; and its level increased after the initiation of adipocyte differentiation, reached its maximum at 1h, and gradually decreased thereafter. Functional suppression of KLF9 mRNA by its siRNAs repressed the accumulation of the intracellular lipids with a reduction in the expression of CCAAT/enhancer-binding protein (C/EBP) β, but not in that of C/EBPδ. In contrast, C/EBPβ and C/EBPδ did not affect the expression of KLF9 in 3T3-L1 cells. A chromatin immunoprecipitation assay revealed that KLF9 bound the KLF binding element at position -874 of the mouse C/EBPβ promoter. Moreover, the ability of KLF9 to bind to this element was enhanced, with a peak at 1-2h after the initiation of adipogenesis, whose profile well resembled that of the expression of the C/EBPβ gene in 3T3-L1 cells. These results indicate that KLF9 activated the early phase of adipogenesis by enhancing the expression of the C/EBPβ gene in 3T3-L1 cells.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kimura H
Laboratory of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Fujimori K
Laboratory of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. Electronic address: fujimori@gly.oups.ac.jp.

MeSH

3T3-L1 CellsAdipocytesAdipogenesisAnimalsCCAAT-Enhancer-Binding Protein-betaCell DifferentiationCell LineDNA-Binding ProteinsKruppel-Like Transcription FactorsMicePromoter Regions, GeneticRNA, Messenger

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24220850

Citation

Kimura, Hiroko, and Ko Fujimori. "Activation of Early Phase of Adipogenesis Through Krüppel-like Factor KLF9-mediated, Enhanced Expression of CCAAT/enhancer-binding Protein Β in 3T3-L1 Cells." Gene, vol. 534, no. 2, 2014, pp. 169-76.
Kimura H, Fujimori K. Activation of early phase of adipogenesis through Krüppel-like factor KLF9-mediated, enhanced expression of CCAAT/enhancer-binding protein β in 3T3-L1 cells. Gene. 2014;534(2):169-76.
Kimura, H., & Fujimori, K. (2014). Activation of early phase of adipogenesis through Krüppel-like factor KLF9-mediated, enhanced expression of CCAAT/enhancer-binding protein β in 3T3-L1 cells. Gene, 534(2), 169-76. https://doi.org/10.1016/j.gene.2013.10.065
Kimura H, Fujimori K. Activation of Early Phase of Adipogenesis Through Krüppel-like Factor KLF9-mediated, Enhanced Expression of CCAAT/enhancer-binding Protein Β in 3T3-L1 Cells. Gene. 2014 Jan 25;534(2):169-76. PubMed PMID: 24220850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of early phase of adipogenesis through Krüppel-like factor KLF9-mediated, enhanced expression of CCAAT/enhancer-binding protein β in 3T3-L1 cells. AU - Kimura,Hiroko, AU - Fujimori,Ko, Y1 - 2013/11/09/ PY - 2013/08/03/received PY - 2013/10/11/revised PY - 2013/10/28/accepted PY - 2013/11/14/entrez PY - 2013/11/14/pubmed PY - 2014/4/18/medline KW - Adipocytes KW - Adipogenesis KW - C/EBP KW - C/EBPβ KW - CCAAT/enhancer-binding protein KW - ChIP KW - FAS KW - KLF KW - KLF-binding element KW - KLFE KW - Krüppel-like factor KW - LPL KW - N.C. KW - PPAR KW - SCD KW - SREBP KW - TATA-binding protein KW - TBP KW - Transcription factor KW - aP2 KW - chromatin immunoprecipitation KW - fatty acid binding protein 4 KW - fatty acid synthase KW - lipoprotein lipase KW - negative control KW - peroxisome proliferator-activated receptor KW - stearoyl-CoA desaturase KW - sterol regulatory element-binding protein SP - 169 EP - 76 JF - Gene JO - Gene VL - 534 IS - 2 N2 - In this study, we found that Krüppel-like factor (KLF) 9 activate the progression of the early phase of adipocyte differentiation in mouse adipocytic 3T3-L1 cells. KLF9 mRNA was detected in preadipocytes; and its level increased after the initiation of adipocyte differentiation, reached its maximum at 1h, and gradually decreased thereafter. Functional suppression of KLF9 mRNA by its siRNAs repressed the accumulation of the intracellular lipids with a reduction in the expression of CCAAT/enhancer-binding protein (C/EBP) β, but not in that of C/EBPδ. In contrast, C/EBPβ and C/EBPδ did not affect the expression of KLF9 in 3T3-L1 cells. A chromatin immunoprecipitation assay revealed that KLF9 bound the KLF binding element at position -874 of the mouse C/EBPβ promoter. Moreover, the ability of KLF9 to bind to this element was enhanced, with a peak at 1-2h after the initiation of adipogenesis, whose profile well resembled that of the expression of the C/EBPβ gene in 3T3-L1 cells. These results indicate that KLF9 activated the early phase of adipogenesis by enhancing the expression of the C/EBPβ gene in 3T3-L1 cells. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24220850/Activation_of_early_phase_of_adipogenesis_through_Krüppel_like_factor_KLF9_mediated_enhanced_expression_of_CCAAT/enhancer_binding_protein_β_in_3T3_L1_cells_ DB - PRIME DP - Unbound Medicine ER -