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Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV/neo.
PLoS One 2013; 8(11):e79598Plos

Abstract

SPRY4-IT1 has been reported to have extremely high expression in normal placenta tissues. It is a Long noncoding RNA (lncRNA), which is associated with cell growth, migration, invasion, and apoptosis in melanoma. A 2.8-fold increase of SPRY4-IT1 expression was validated by Real-time reverse transcription-polymerase chain reaction (qRT-PCR) in severe preeclamptic placenta as compared with that of the normal ones (n=25) in this study. Furthermore, the role of SPRY4-IT1 in proliferation, migration, apoptosis, and network formation ability of trophoblast cells HTR-8/SVneo was assessed. Suppression of SPRY4-IT1 using siRNA treatment and its overexpression using plasmid targeting SPRY4-IT1 were performed in order to explore the biological function of SPRY4-IT1 in the development and progression of trophoblast cells HTR-8/SVneo, in vitro. The results showed that SPRY4-IT1 knockdown enhanced the cell migration and proliferation, and reduced the response of cells to apoptosis. However, exogenous SPRY4-IT1 overexpression significantly decreased the cell migration and proliferation, while increased cell apoptosis. Our study showed for the first time that aberrant expression of lncRNA SPRY4-IT1 might contribute to the abnormal condition of trophoblast cells HTR-8/SVneo. Therefore, we proposed SPRY4-IT1 as a novel lncRNA molecule, which might be associated with the pathogenesis of preeclampsia and might provide a new target for its early diagnosis and treatment.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24223182

Citation

Zou, Yanfen, et al. "Upregulation of Long Noncoding RNA SPRY4-IT1 Modulates Proliferation, Migration, Apoptosis, and Network Formation in Trophoblast Cells HTR-8SV/neo." PloS One, vol. 8, no. 11, 2013, pp. e79598.
Zou Y, Jiang Z, Yu X, et al. Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV/neo. PLoS ONE. 2013;8(11):e79598.
Zou, Y., Jiang, Z., Yu, X., Sun, M., Zhang, Y., Zuo, Q., ... Sun, L. (2013). Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV/neo. PloS One, 8(11), pp. e79598. doi:10.1371/journal.pone.0079598.
Zou Y, et al. Upregulation of Long Noncoding RNA SPRY4-IT1 Modulates Proliferation, Migration, Apoptosis, and Network Formation in Trophoblast Cells HTR-8SV/neo. PLoS ONE. 2013;8(11):e79598. PubMed PMID: 24223182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of long noncoding RNA SPRY4-IT1 modulates proliferation, migration, apoptosis, and network formation in trophoblast cells HTR-8SV/neo. AU - Zou,Yanfen, AU - Jiang,Ziyan, AU - Yu,Xiang, AU - Sun,Ming, AU - Zhang,Yuanyuan, AU - Zuo,Qing, AU - Zhou,Jing, AU - Yang,Nana, AU - Han,Ping, AU - Ge,Zhiping, AU - De,Wei, AU - Sun,Lizhou, Y1 - 2013/11/06/ PY - 2013/04/19/received PY - 2013/09/24/accepted PY - 2013/11/14/entrez PY - 2013/11/14/pubmed PY - 2014/8/19/medline SP - e79598 EP - e79598 JF - PloS one JO - PLoS ONE VL - 8 IS - 11 N2 - SPRY4-IT1 has been reported to have extremely high expression in normal placenta tissues. It is a Long noncoding RNA (lncRNA), which is associated with cell growth, migration, invasion, and apoptosis in melanoma. A 2.8-fold increase of SPRY4-IT1 expression was validated by Real-time reverse transcription-polymerase chain reaction (qRT-PCR) in severe preeclamptic placenta as compared with that of the normal ones (n=25) in this study. Furthermore, the role of SPRY4-IT1 in proliferation, migration, apoptosis, and network formation ability of trophoblast cells HTR-8/SVneo was assessed. Suppression of SPRY4-IT1 using siRNA treatment and its overexpression using plasmid targeting SPRY4-IT1 were performed in order to explore the biological function of SPRY4-IT1 in the development and progression of trophoblast cells HTR-8/SVneo, in vitro. The results showed that SPRY4-IT1 knockdown enhanced the cell migration and proliferation, and reduced the response of cells to apoptosis. However, exogenous SPRY4-IT1 overexpression significantly decreased the cell migration and proliferation, while increased cell apoptosis. Our study showed for the first time that aberrant expression of lncRNA SPRY4-IT1 might contribute to the abnormal condition of trophoblast cells HTR-8/SVneo. Therefore, we proposed SPRY4-IT1 as a novel lncRNA molecule, which might be associated with the pathogenesis of preeclampsia and might provide a new target for its early diagnosis and treatment. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24223182/Upregulation_of_long_noncoding_RNA_SPRY4_IT1_modulates_proliferation_migration_apoptosis_and_network_formation_in_trophoblast_cells_HTR_8SV/neo_ L2 - http://dx.plos.org/10.1371/journal.pone.0079598 DB - PRIME DP - Unbound Medicine ER -