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A mutation (L1014F) in the voltage-gated sodium channel of the grain aphid, Sitobion avenae, is associated with resistance to pyrethroid insecticides.
Pest Manag Sci. 2014 Aug; 70(8):1249-53.PM

Abstract

BACKGROUND

The grain aphid, Sitobion avenae Fabricius (Hemiptera: Aphididae), is an important pest of cereal crops. Pesticides are the main method for control but carry the risk of selecting for resistance. In response to reports of reduced efficacy of pyrethroid sprays applied to S. avenae, field samples were collected and screened for mutations in the voltage-gated sodium channel, the primary target site for pyrethroids. Aphid mobility and mortality to lambda-cyhalothrin were measured in coated glass vial bioassays.

RESULTS

A single amino acid substitution (L1014F) was identified in the domain IIS6 segment of the sodium channel from the S. avenae samples exhibiting reduced pyrethroid efficacy. Bioassays on aphids heterozygous for the kdr mutation (SR) or homozygous for the wild-type allele (SS) showed that those carrying the mutation had significantly lower susceptibility to lambda-cyhalothrin.

CONCLUSION

The L1014F (kdr) mutation, known to confer pyrethroid resistance in many insect pests, has been identified for the first time in S. avenae. Clonal lines heterozygous for the mutation showed 35-40-fold resistance to lambda-cyhalothrin in laboratory bioassays, consistent with the reported effect of this mutation on pyrethroid sensitivity in other aphid species.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Foster SP
Rothamsted Research, Harpenden, Hertfordshire, UK.
Paul VL
No affiliation info available
Slater R
No affiliation info available
Warren A
No affiliation info available
Denholm I
No affiliation info available
Field LM
No affiliation info available
Williamson MS
No affiliation info available

MeSH

Amino Acid SubstitutionAnimalsAphidsBase SequenceBiological AssayEdible GrainEnglandInsecticide ResistanceInsecticidesMolecular Sequence DataNitrilesPyrethrinsSodium Channels

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24227679

Citation

Foster, Stephen P., et al. "A Mutation (L1014F) in the Voltage-gated Sodium Channel of the Grain Aphid, Sitobion Avenae, Is Associated With Resistance to Pyrethroid Insecticides." Pest Management Science, vol. 70, no. 8, 2014, pp. 1249-53.
Foster SP, Paul VL, Slater R, et al. A mutation (L1014F) in the voltage-gated sodium channel of the grain aphid, Sitobion avenae, is associated with resistance to pyrethroid insecticides. Pest Manag Sci. 2014;70(8):1249-53.
Foster, S. P., Paul, V. L., Slater, R., Warren, A., Denholm, I., Field, L. M., & Williamson, M. S. (2014). A mutation (L1014F) in the voltage-gated sodium channel of the grain aphid, Sitobion avenae, is associated with resistance to pyrethroid insecticides. Pest Management Science, 70(8), 1249-53. https://doi.org/10.1002/ps.3683
Foster SP, et al. A Mutation (L1014F) in the Voltage-gated Sodium Channel of the Grain Aphid, Sitobion Avenae, Is Associated With Resistance to Pyrethroid Insecticides. Pest Manag Sci. 2014;70(8):1249-53. PubMed PMID: 24227679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mutation (L1014F) in the voltage-gated sodium channel of the grain aphid, Sitobion avenae, is associated with resistance to pyrethroid insecticides. AU - Foster,Stephen P, AU - Paul,Verity L, AU - Slater,Russell, AU - Warren,Anne, AU - Denholm,Ian, AU - Field,Linda M, AU - Williamson,Martin S, Y1 - 2013/12/12/ PY - 2013/09/10/received PY - 2013/10/30/revised PY - 2013/11/14/accepted PY - 2013/11/15/entrez PY - 2013/11/15/pubmed PY - 2015/3/5/medline KW - Sitobion avenae KW - grain aphid KW - knockdown resistance KW - mutation KW - pyrethroid KW - voltage-gated sodium channel SP - 1249 EP - 53 JF - Pest management science JO - Pest Manag Sci VL - 70 IS - 8 N2 - BACKGROUND: The grain aphid, Sitobion avenae Fabricius (Hemiptera: Aphididae), is an important pest of cereal crops. Pesticides are the main method for control but carry the risk of selecting for resistance. In response to reports of reduced efficacy of pyrethroid sprays applied to S. avenae, field samples were collected and screened for mutations in the voltage-gated sodium channel, the primary target site for pyrethroids. Aphid mobility and mortality to lambda-cyhalothrin were measured in coated glass vial bioassays. RESULTS: A single amino acid substitution (L1014F) was identified in the domain IIS6 segment of the sodium channel from the S. avenae samples exhibiting reduced pyrethroid efficacy. Bioassays on aphids heterozygous for the kdr mutation (SR) or homozygous for the wild-type allele (SS) showed that those carrying the mutation had significantly lower susceptibility to lambda-cyhalothrin. CONCLUSION: The L1014F (kdr) mutation, known to confer pyrethroid resistance in many insect pests, has been identified for the first time in S. avenae. Clonal lines heterozygous for the mutation showed 35-40-fold resistance to lambda-cyhalothrin in laboratory bioassays, consistent with the reported effect of this mutation on pyrethroid sensitivity in other aphid species. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/24227679/A_mutation__L1014F__in_the_voltage_gated_sodium_channel_of_the_grain_aphid_Sitobion_avenae_is_associated_with_resistance_to_pyrethroid_insecticides_ DB - PRIME DP - Unbound Medicine ER -
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