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Electroacupuncture at ST36 increases contraction of the gastric antrum and improves the SCF/c-kit pathway in diabetic rats.
Am J Chin Med. 2013; 41(6):1233-49.AJ

Abstract

Electroacupuncture (EA) at ST36 is effective for improving gastric motility. However, the underlying mechanism remains poorly understood. The aim of this study was to investigate the effects of EA on gastric contraction and to determine whether interstitial cells of Cajal (ICCs) are involved. Rats were randomized into control, diabetic (DM), diabetic with sham EA (DM + SEA), diabetic with low frequency EA (DM + LEA) and diabetic with high frequency EA (DM + HEA) groups. EA was performed everyday for four and eight weeks. Contractions in antrum strips were explored using the organ bath technique. Western blotting was employed to determine c-kit and transmembrane stem cell factor (M-SCF) expression in the gastric antrum, and levels of soluble stem cell factor (S-SCF) in serum were determined by enzyme-linked immunosorbent assay (ELISA). The distribution of ICCs was further assessed by immunohistochemistry. The results were as follows: (1) Contractions in the DM group were attenuated at four and eight weeks, but LEA and HEA restored the attenuated contraction. (2) ICCs were significantly decreased at eight weeks without alteration at four weeks in DM group, but were rescued in the LEA and HEA groups. (3) Whereas M-SCF and S-SCF in the DM group were slightly decreased at four weeks and were dramatically reduced at eight weeks, LEA and HEA markedly enhanced SCF at eight weeks. Collectively, the data suggest that in diabetic rats, LEA and HEA at ST36 could facilitate contraction of the gastric antrum, possibly by involving the SCF/c-kit pathway.

Authors+Show Affiliations

Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24228598

Citation

Chen, Yan, et al. "Electroacupuncture at ST36 Increases Contraction of the Gastric Antrum and Improves the SCF/c-kit Pathway in Diabetic Rats." The American Journal of Chinese Medicine, vol. 41, no. 6, 2013, pp. 1233-49.
Chen Y, Xu J, Liu S, et al. Electroacupuncture at ST36 increases contraction of the gastric antrum and improves the SCF/c-kit pathway in diabetic rats. Am J Chin Med. 2013;41(6):1233-49.
Chen, Y., Xu, J., Liu, S., & Hou, X. (2013). Electroacupuncture at ST36 increases contraction of the gastric antrum and improves the SCF/c-kit pathway in diabetic rats. The American Journal of Chinese Medicine, 41(6), 1233-49. https://doi.org/10.1142/S0192415X13500833
Chen Y, et al. Electroacupuncture at ST36 Increases Contraction of the Gastric Antrum and Improves the SCF/c-kit Pathway in Diabetic Rats. Am J Chin Med. 2013;41(6):1233-49. PubMed PMID: 24228598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electroacupuncture at ST36 increases contraction of the gastric antrum and improves the SCF/c-kit pathway in diabetic rats. AU - Chen,Yan, AU - Xu,Juanjuan, AU - Liu,Shi, AU - Hou,Xiaohua, PY - 2013/11/16/entrez PY - 2013/11/16/pubmed PY - 2014/7/2/medline SP - 1233 EP - 49 JF - The American journal of Chinese medicine JO - Am J Chin Med VL - 41 IS - 6 N2 - Electroacupuncture (EA) at ST36 is effective for improving gastric motility. However, the underlying mechanism remains poorly understood. The aim of this study was to investigate the effects of EA on gastric contraction and to determine whether interstitial cells of Cajal (ICCs) are involved. Rats were randomized into control, diabetic (DM), diabetic with sham EA (DM + SEA), diabetic with low frequency EA (DM + LEA) and diabetic with high frequency EA (DM + HEA) groups. EA was performed everyday for four and eight weeks. Contractions in antrum strips were explored using the organ bath technique. Western blotting was employed to determine c-kit and transmembrane stem cell factor (M-SCF) expression in the gastric antrum, and levels of soluble stem cell factor (S-SCF) in serum were determined by enzyme-linked immunosorbent assay (ELISA). The distribution of ICCs was further assessed by immunohistochemistry. The results were as follows: (1) Contractions in the DM group were attenuated at four and eight weeks, but LEA and HEA restored the attenuated contraction. (2) ICCs were significantly decreased at eight weeks without alteration at four weeks in DM group, but were rescued in the LEA and HEA groups. (3) Whereas M-SCF and S-SCF in the DM group were slightly decreased at four weeks and were dramatically reduced at eight weeks, LEA and HEA markedly enhanced SCF at eight weeks. Collectively, the data suggest that in diabetic rats, LEA and HEA at ST36 could facilitate contraction of the gastric antrum, possibly by involving the SCF/c-kit pathway. SN - 1793-6853 UR - https://www.unboundmedicine.com/medline/citation/24228598/Electroacupuncture_at_ST36_increases_contraction_of_the_gastric_antrum_and_improves_the_SCF/c_kit_pathway_in_diabetic_rats_ L2 - https://www.worldscientific.com/doi/10.1142/S0192415X13500833?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -