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[Effect of Mycobacterium phlei F.U.36 on balance of CD4⁺CD25⁺ regulatory T cells and Th17 cells in asthmatic mice].
Zhongguo Dang Dai Er Ke Za Zhi. 2013 Nov; 15(11):1018-22.ZD

Abstract

OBJECTIVE

To evaluate the effect of early intervention with Mycobacterium phlei F.U.36 injection on the balance of CD4⁺CD25⁺ regulatory T cells and Th17 cells in asthmatic mice, and to investigate the immunomodulatory effect of Mycobacterium phlei F.U.36.

METHODS

Thirty female BALB/c mice were randomly divided into three groups: normal control (n=10), asthma model (n=10) and Mycobacterium phlei F.U.36 treatment groups (n=10). A mouse model of asthma was prepared by injection and aerosol inhalation of chicken ovalbumin in the asthma model and Mycobacterium phlei F.U.36 treatment groups, while mice in the normal control group were given normal saline instead. The treatment group was intraperitoneally injected with Mycobacterium phlei F.U.36 (0.57 μg, once every other day) three times in the first two weeks after the first sensitization. All mice were sacrificed at 24 hours after the last challenge. Left lung tissues of these mice were obtained and made into sections for observation of inflammatory changes. The percentages of CD4⁺CD25⁺ regulatory T cells and Th17 cells in CD4⁺ T cells among splenic mononuclear cells were determined by flow cytometry. The levels of interleukin (IL)-10 and IL-17 in serum and bronchoalveolar lavage fluid were measured using ELISA.

RESULTS

Compared with the normal control group, the asthma model group had significantly decreased percentages of CD4⁺CD25⁺ regulatory T cells and IL-10 levels (P<0.05) and significantly increased percentages of Th17 cells and IL-17 levels (P<0.05). Compared with the asthma model group, the Mycobacterium phlei F.U.36 treatment group had significantly increased percentages of CD4⁺CD25⁺ regulatory T cells and IL-10 levels (P<0.05) and significantly decreased percentage of Th17 cells and IL-17 levels (P<0.05).

CONCLUSIONS

Early intervention with Mycobacterium phlei F.U.36 can promote development of CD4⁺CD25⁺ regulatory T cells and production of IL-10 and inhibit generation of Th17 cells and production of IL-17 in asthmatic mice.

Authors+Show Affiliations

Department of Pediatrics, East Ward, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu 610110, China. Lmscsy@yahoo.com.cn.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

24229602

Citation

Yao, Bin, et al. "[Effect of Mycobacterium Phlei F.U.36 On Balance of CD4⁺CD25⁺ Regulatory T Cells and Th17 Cells in Asthmatic Mice]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 15, no. 11, 2013, pp. 1018-22.
Yao B, Li M, Pang Y. [Effect of Mycobacterium phlei F.U.36 on balance of CD4⁺CD25⁺ regulatory T cells and Th17 cells in asthmatic mice]. Zhongguo Dang Dai Er Ke Za Zhi. 2013;15(11):1018-22.
Yao, B., Li, M., & Pang, Y. (2013). [Effect of Mycobacterium phlei F.U.36 on balance of CD4⁺CD25⁺ regulatory T cells and Th17 cells in asthmatic mice]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 15(11), 1018-22.
Yao B, Li M, Pang Y. [Effect of Mycobacterium Phlei F.U.36 On Balance of CD4⁺CD25⁺ Regulatory T Cells and Th17 Cells in Asthmatic Mice]. Zhongguo Dang Dai Er Ke Za Zhi. 2013;15(11):1018-22. PubMed PMID: 24229602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Mycobacterium phlei F.U.36 on balance of CD4⁺CD25⁺ regulatory T cells and Th17 cells in asthmatic mice]. AU - Yao,Bin, AU - Li,Min, AU - Pang,Ying, PY - 2013/11/16/entrez PY - 2013/11/16/pubmed PY - 2014/3/26/medline SP - 1018 EP - 22 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 15 IS - 11 N2 - OBJECTIVE: To evaluate the effect of early intervention with Mycobacterium phlei F.U.36 injection on the balance of CD4⁺CD25⁺ regulatory T cells and Th17 cells in asthmatic mice, and to investigate the immunomodulatory effect of Mycobacterium phlei F.U.36. METHODS: Thirty female BALB/c mice were randomly divided into three groups: normal control (n=10), asthma model (n=10) and Mycobacterium phlei F.U.36 treatment groups (n=10). A mouse model of asthma was prepared by injection and aerosol inhalation of chicken ovalbumin in the asthma model and Mycobacterium phlei F.U.36 treatment groups, while mice in the normal control group were given normal saline instead. The treatment group was intraperitoneally injected with Mycobacterium phlei F.U.36 (0.57 μg, once every other day) three times in the first two weeks after the first sensitization. All mice were sacrificed at 24 hours after the last challenge. Left lung tissues of these mice were obtained and made into sections for observation of inflammatory changes. The percentages of CD4⁺CD25⁺ regulatory T cells and Th17 cells in CD4⁺ T cells among splenic mononuclear cells were determined by flow cytometry. The levels of interleukin (IL)-10 and IL-17 in serum and bronchoalveolar lavage fluid were measured using ELISA. RESULTS: Compared with the normal control group, the asthma model group had significantly decreased percentages of CD4⁺CD25⁺ regulatory T cells and IL-10 levels (P<0.05) and significantly increased percentages of Th17 cells and IL-17 levels (P<0.05). Compared with the asthma model group, the Mycobacterium phlei F.U.36 treatment group had significantly increased percentages of CD4⁺CD25⁺ regulatory T cells and IL-10 levels (P<0.05) and significantly decreased percentage of Th17 cells and IL-17 levels (P<0.05). CONCLUSIONS: Early intervention with Mycobacterium phlei F.U.36 can promote development of CD4⁺CD25⁺ regulatory T cells and production of IL-10 and inhibit generation of Th17 cells and production of IL-17 in asthmatic mice. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/24229602/[Effect_of_Mycobacterium_phlei_F_U_36_on_balance_of_CD4⁺CD25⁺_regulatory_T_cells_and_Th17_cells_in_asthmatic_mice]_ DB - PRIME DP - Unbound Medicine ER -