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The effects of dietary fatty acids on the postprandial triglyceride-rich lipoprotein/apoB48 receptor axis in human monocyte/macrophage cells.
J Nutr Biochem. 2013 Dec; 24(12):2031-9.JN

Abstract

Intestinally produced triglyceride-rich lipoproteins (TRL) play an important role in the progression of atherosclerosis. In this study, we investigated the relevance of monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) in postprandial TRL in affecting the transcriptional activity of the apolipoprotein-B48 receptor (ApoB48R) and its functionality in human monocyte/macrophage cells. Healthy male volunteers were administered four standardized high-fat meals containing butter, high-palmitic sunflower oil, olive oil (ROO) or a mixture of vegetable and fish oils (50 g/m(2) body surface area) to obtain a panel of postprandial TRL with gradual MUFA oleic acid-to-SFA palmitic acid ratios. The increase in this ratio was linearly associated with a decrease of ApoB48R up-regulation and lipid accumulation in THP-1 and primary monocytes. ApoB48R mRNA levels and intracellular triglycerides were also lower in the monocytes from volunteers after the ingestion of the ROO meal when compared to the ingestion of the butter meal. In THP-1 macrophages, the increase in the MUFA oleic acid-to-SFA palmitic acid ratio in the postprandial TRL was linearly correlated with an increase in ApoB48R down-regulation and a decrease in lipid accumulation. We also revealed that the nuclear receptor transcription factors PPARα, PPARβ/δ, and PPARγ and the PPAR-RXR transcriptional complex were involved in sensing the proportion of MUFA oleic acid and SFA palmitic acid, and these were also involved in adjusting the transcriptional activity of ApoB48R. The results of this study support the notion that MUFA-rich dietary fats may prevent excessive lipid accumulation in monocyte/macrophage cells by targeting the postprandial TRL/ApoB48R axis.

Authors+Show Affiliations

Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), 41012 Seville, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24231096

Citation

Varela, Lourdes M., et al. "The Effects of Dietary Fatty Acids On the Postprandial Triglyceride-rich lipoprotein/apoB48 Receptor Axis in Human Monocyte/macrophage Cells." The Journal of Nutritional Biochemistry, vol. 24, no. 12, 2013, pp. 2031-9.
Varela LM, Ortega-Gomez A, Lopez S, et al. The effects of dietary fatty acids on the postprandial triglyceride-rich lipoprotein/apoB48 receptor axis in human monocyte/macrophage cells. J Nutr Biochem. 2013;24(12):2031-9.
Varela, L. M., Ortega-Gomez, A., Lopez, S., Abia, R., Muriana, F. J., & Bermudez, B. (2013). The effects of dietary fatty acids on the postprandial triglyceride-rich lipoprotein/apoB48 receptor axis in human monocyte/macrophage cells. The Journal of Nutritional Biochemistry, 24(12), 2031-9. https://doi.org/10.1016/j.jnutbio.2013.07.004
Varela LM, et al. The Effects of Dietary Fatty Acids On the Postprandial Triglyceride-rich lipoprotein/apoB48 Receptor Axis in Human Monocyte/macrophage Cells. J Nutr Biochem. 2013;24(12):2031-9. PubMed PMID: 24231096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of dietary fatty acids on the postprandial triglyceride-rich lipoprotein/apoB48 receptor axis in human monocyte/macrophage cells. AU - Varela,Lourdes M, AU - Ortega-Gomez,Almudena, AU - Lopez,Sergio, AU - Abia,Rocio, AU - Muriana,Francisco J G, AU - Bermudez,Beatriz, PY - 2013/02/05/received PY - 2013/06/10/revised PY - 2013/07/03/accepted PY - 2013/11/16/entrez PY - 2013/11/16/pubmed PY - 2014/6/26/medline KW - 9-cis-retinoic acid KW - 9cRA KW - ABCA1 KW - ACAT KW - ATP-binding cassette transporter A1 KW - ApoB48R KW - Apolipoprotein-B48 receptor KW - C-terminal helix KW - H KW - HPRT KW - HPSO KW - LBD KW - LBP KW - MUFA KW - MUFA oleic acid KW - Macrophages KW - Monocytes KW - PMA KW - PPAR KW - PPRE KW - Postprandial triglyceride-rich lipoproteins KW - ROO KW - RPLP0 KW - RXR KW - SFA KW - SFA palmitic acid KW - TRL KW - VEFO KW - acyl-CoA:cholesterol acyltransferase KW - apolipoprotein-B48 receptor KW - high-palmitic sunflower oil KW - hypoxanthine phosphoribosyltransferase KW - ligand binding domain KW - ligand binding pocket KW - mixture of vegetable and fish oils KW - monounsaturated fatty acids KW - olive oil KW - peroxisome proliferated activated receptor KW - peroxisome proliferator responsive elements KW - phorbol 12-myristate-13-acetate KW - qRT-PCR KW - quantitative reverse transcription polymerase chain reaction KW - retinoid X receptor KW - ribosomal protein large P0 KW - saturated fatty acids KW - siRNA KW - small interfering RNA KW - triglyceride-rich lipoproteins SP - 2031 EP - 9 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 24 IS - 12 N2 - Intestinally produced triglyceride-rich lipoproteins (TRL) play an important role in the progression of atherosclerosis. In this study, we investigated the relevance of monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) in postprandial TRL in affecting the transcriptional activity of the apolipoprotein-B48 receptor (ApoB48R) and its functionality in human monocyte/macrophage cells. Healthy male volunteers were administered four standardized high-fat meals containing butter, high-palmitic sunflower oil, olive oil (ROO) or a mixture of vegetable and fish oils (50 g/m(2) body surface area) to obtain a panel of postprandial TRL with gradual MUFA oleic acid-to-SFA palmitic acid ratios. The increase in this ratio was linearly associated with a decrease of ApoB48R up-regulation and lipid accumulation in THP-1 and primary monocytes. ApoB48R mRNA levels and intracellular triglycerides were also lower in the monocytes from volunteers after the ingestion of the ROO meal when compared to the ingestion of the butter meal. In THP-1 macrophages, the increase in the MUFA oleic acid-to-SFA palmitic acid ratio in the postprandial TRL was linearly correlated with an increase in ApoB48R down-regulation and a decrease in lipid accumulation. We also revealed that the nuclear receptor transcription factors PPARα, PPARβ/δ, and PPARγ and the PPAR-RXR transcriptional complex were involved in sensing the proportion of MUFA oleic acid and SFA palmitic acid, and these were also involved in adjusting the transcriptional activity of ApoB48R. The results of this study support the notion that MUFA-rich dietary fats may prevent excessive lipid accumulation in monocyte/macrophage cells by targeting the postprandial TRL/ApoB48R axis. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/24231096/The_effects_of_dietary_fatty_acids_on_the_postprandial_triglyceride_rich_lipoprotein/apoB48_receptor_axis_in_human_monocyte/macrophage_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(13)00148-4 DB - PRIME DP - Unbound Medicine ER -