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Characterization of the vaginal microbiota among sexual risk behavior groups of women with bacterial vaginosis.
PLoS One. 2013; 8(11):e80254.Plos

Abstract

BACKGROUND

The pathogenesis of bacterial vaginosis (BV) remains elusive. BV may be more common among women who have sex with women (WSW). The objective of this study was to use 454 pyrosequencing to investigate the vaginal microbiome of WSW, women who have sex with women and men (WSWM), and women who have sex with men (WSM) with BV to determine if there are differences in organism composition between groups that may inform new hypotheses regarding the pathogenesis of BV.

METHODS

Vaginal swab specimens from eligible women with BV at the Mississippi State Department of Health STD Clinic were used. After DNA extraction, 454 pyrosequencing of PCR-amplified 16S rRNA gene sequences was performed. Sequence data was classified using the Ribosomal Database Program classifer. Complete linkage clustering analysis was performed to compare bacterial community composition among samples. Differences in operational taxonomic units with an abundance of ≥ 2% between risk behavior groups were determined. Alpha and beta diversity were measured using Shannon's Index implemented in QIIME and Unifrac analysis, respectively.

RESULTS

33 WSW, 35 WSWM, and 44 WSM were included. The vaginal bacterial communities of all women clustered into four taxonomic groups with the dominant taxonomic group in each being Lactobacillus, Lachnospiraceae, Prevotella, and Sneathia. Regarding differences in organism composition between risk behavior groups, the abundance of Atopobium (relative ratio (RR)=0.24; 95%CI 0.11-0.54) and Parvimonas (RR=0.33; 95%CI 0.11-0.93) were significantly lower in WSW than WSM, the abundance of Prevotella was significantly higher in WSW than WSWM (RR=1.77; 95%CI 1.10-2.86), and the abundance of Atopobium (RR=0.41; 95%CI 0.18-0.88) was significantly lower in WSWM than WSM. Overall, WSM had the highest diversity of bacterial taxa.

CONCLUSION

The microbiology of BV among women in different risk behavior groups is heterogeneous. WSM in this study had the highest diversity of bacterial taxa. Additional studies are needed to better understand these differences.

Authors+Show Affiliations

Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, Alabama, United States of America.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24236175

Citation

Muzny, Christina A., et al. "Characterization of the Vaginal Microbiota Among Sexual Risk Behavior Groups of Women With Bacterial Vaginosis." PloS One, vol. 8, no. 11, 2013, pp. e80254.
Muzny CA, Sunesara IR, Kumar R, et al. Characterization of the vaginal microbiota among sexual risk behavior groups of women with bacterial vaginosis. PLoS One. 2013;8(11):e80254.
Muzny, C. A., Sunesara, I. R., Kumar, R., Mena, L. A., Griswold, M. E., Martin, D. H., Lefkowitz, E. J., Schwebke, J. R., & Swiatlo, E. (2013). Characterization of the vaginal microbiota among sexual risk behavior groups of women with bacterial vaginosis. PloS One, 8(11), e80254. https://doi.org/10.1371/journal.pone.0080254
Muzny CA, et al. Characterization of the Vaginal Microbiota Among Sexual Risk Behavior Groups of Women With Bacterial Vaginosis. PLoS One. 2013;8(11):e80254. PubMed PMID: 24236175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the vaginal microbiota among sexual risk behavior groups of women with bacterial vaginosis. AU - Muzny,Christina A, AU - Sunesara,Imran R, AU - Kumar,Ranjit, AU - Mena,Leandro A, AU - Griswold,Michael E, AU - Martin,David H, AU - Lefkowitz,Elliot J, AU - Schwebke,Jane R, AU - Swiatlo,Edwin, Y1 - 2013/11/13/ PY - 2013/06/04/received PY - 2013/10/01/accepted PY - 2013/11/16/entrez PY - 2013/11/16/pubmed PY - 2014/7/26/medline SP - e80254 EP - e80254 JF - PloS one JO - PLoS One VL - 8 IS - 11 N2 - BACKGROUND: The pathogenesis of bacterial vaginosis (BV) remains elusive. BV may be more common among women who have sex with women (WSW). The objective of this study was to use 454 pyrosequencing to investigate the vaginal microbiome of WSW, women who have sex with women and men (WSWM), and women who have sex with men (WSM) with BV to determine if there are differences in organism composition between groups that may inform new hypotheses regarding the pathogenesis of BV. METHODS: Vaginal swab specimens from eligible women with BV at the Mississippi State Department of Health STD Clinic were used. After DNA extraction, 454 pyrosequencing of PCR-amplified 16S rRNA gene sequences was performed. Sequence data was classified using the Ribosomal Database Program classifer. Complete linkage clustering analysis was performed to compare bacterial community composition among samples. Differences in operational taxonomic units with an abundance of ≥ 2% between risk behavior groups were determined. Alpha and beta diversity were measured using Shannon's Index implemented in QIIME and Unifrac analysis, respectively. RESULTS: 33 WSW, 35 WSWM, and 44 WSM were included. The vaginal bacterial communities of all women clustered into four taxonomic groups with the dominant taxonomic group in each being Lactobacillus, Lachnospiraceae, Prevotella, and Sneathia. Regarding differences in organism composition between risk behavior groups, the abundance of Atopobium (relative ratio (RR)=0.24; 95%CI 0.11-0.54) and Parvimonas (RR=0.33; 95%CI 0.11-0.93) were significantly lower in WSW than WSM, the abundance of Prevotella was significantly higher in WSW than WSWM (RR=1.77; 95%CI 1.10-2.86), and the abundance of Atopobium (RR=0.41; 95%CI 0.18-0.88) was significantly lower in WSWM than WSM. Overall, WSM had the highest diversity of bacterial taxa. CONCLUSION: The microbiology of BV among women in different risk behavior groups is heterogeneous. WSM in this study had the highest diversity of bacterial taxa. Additional studies are needed to better understand these differences. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24236175/Characterization_of_the_vaginal_microbiota_among_sexual_risk_behavior_groups_of_women_with_bacterial_vaginosis_ L2 - https://dx.plos.org/10.1371/journal.pone.0080254 DB - PRIME DP - Unbound Medicine ER -