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Common variable immunodeficiency revisited: normal generation of naturally occurring dendritic cells that respond to Toll-like receptors 7 and 9.
Clin Exp Immunol. 2014 Mar; 175(3):439-48.CE

Abstract

Patients with common variable immunodeficiency (CVID) have reduced numbers and frequencies of dendritic cells (DCs) in blood, and there is also evidence for defective activation through Toll-like receptors (TLRs). Collectively, these observations may point to a primary defect in the generation of functional DCs. Here, we measured frequencies of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in peripheral blood of 26 CVID patients and 16 healthy controls. The results show that the patients have reduced absolute counts of both subsets. However, the decreased numbers in peripheral blood were not reflected in reduced frequencies of CD34(+) pDC progenitors in the bone marrow. Moreover, studies at the single cell level showed that DCs from CVID patients and healthy controls produced similar amounts of interferon-α or interleukin-12 and expressed similar levels of activation markers in response to human cytomegalovirus and ligands for TLR-7 and TLR-9. The study represents the most thorough functional characterization to date, and the first to assess bone marrow progenitor output, of naturally occurring DCs in CVID. In conclusion, it seems unlikely that CVID is secondary to insufficient production of naturally occurring DCs or a defect in their signalling through TLR-7 or TLR-9.

Authors+Show Affiliations

Department of Immunology, Institute for Cancer Research, Oslo University Hospital Radiumhospitalet, Oslo, Norway; K.G. Jebsen Center for Cancer Immunotherapy and K.G. Jebsen Inflammation Research Center, University of Oslo, Oslo, Norway; Research Institute of Internal Medicine, Oslo University Hospital Rikshospitalet, Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24237110

Citation

Taraldsrud, E, et al. "Common Variable Immunodeficiency Revisited: Normal Generation of Naturally Occurring Dendritic Cells That Respond to Toll-like Receptors 7 and 9." Clinical and Experimental Immunology, vol. 175, no. 3, 2014, pp. 439-48.
Taraldsrud E, Fevang B, Aukrust P, et al. Common variable immunodeficiency revisited: normal generation of naturally occurring dendritic cells that respond to Toll-like receptors 7 and 9. Clin Exp Immunol. 2014;175(3):439-48.
Taraldsrud, E., Fevang, B., Aukrust, P., Beiske, K. H., Fløisand, Y., Frøland, S., Rollag, H., & Olweus, J. (2014). Common variable immunodeficiency revisited: normal generation of naturally occurring dendritic cells that respond to Toll-like receptors 7 and 9. Clinical and Experimental Immunology, 175(3), 439-48. https://doi.org/10.1111/cei.12239
Taraldsrud E, et al. Common Variable Immunodeficiency Revisited: Normal Generation of Naturally Occurring Dendritic Cells That Respond to Toll-like Receptors 7 and 9. Clin Exp Immunol. 2014;175(3):439-48. PubMed PMID: 24237110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Common variable immunodeficiency revisited: normal generation of naturally occurring dendritic cells that respond to Toll-like receptors 7 and 9. AU - Taraldsrud,E, AU - Fevang,B, AU - Aukrust,P, AU - Beiske,K H, AU - Fløisand,Y, AU - Frøland,S, AU - Rollag,H, AU - Olweus,J, PY - 2013/11/12/accepted PY - 2013/11/19/entrez PY - 2013/11/19/pubmed PY - 2014/4/9/medline KW - IFN-α KW - TLR-9 KW - bone marrow KW - common variable immunodeficiency KW - plasmacytoid dendritic cell SP - 439 EP - 48 JF - Clinical and experimental immunology JO - Clin. Exp. Immunol. VL - 175 IS - 3 N2 - Patients with common variable immunodeficiency (CVID) have reduced numbers and frequencies of dendritic cells (DCs) in blood, and there is also evidence for defective activation through Toll-like receptors (TLRs). Collectively, these observations may point to a primary defect in the generation of functional DCs. Here, we measured frequencies of plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) in peripheral blood of 26 CVID patients and 16 healthy controls. The results show that the patients have reduced absolute counts of both subsets. However, the decreased numbers in peripheral blood were not reflected in reduced frequencies of CD34(+) pDC progenitors in the bone marrow. Moreover, studies at the single cell level showed that DCs from CVID patients and healthy controls produced similar amounts of interferon-α or interleukin-12 and expressed similar levels of activation markers in response to human cytomegalovirus and ligands for TLR-7 and TLR-9. The study represents the most thorough functional characterization to date, and the first to assess bone marrow progenitor output, of naturally occurring DCs in CVID. In conclusion, it seems unlikely that CVID is secondary to insufficient production of naturally occurring DCs or a defect in their signalling through TLR-7 or TLR-9. SN - 1365-2249 UR - https://www.unboundmedicine.com/medline/citation/24237110/Common_variable_immunodeficiency_revisited:_normal_generation_of_naturally_occurring_dendritic_cells_that_respond_to_Toll_like_receptors_7_and_9_ L2 - https://doi.org/10.1111/cei.12239 DB - PRIME DP - Unbound Medicine ER -