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Hypothermic machine perfusion versus cold storage in the rescuing of livers from non-heart-beating donor rats.
Artif Organs 2013; 37(11):985-91AO

Abstract

The aim of this work was to compare the efficiency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs-Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS.

Authors+Show Affiliations

Binational Center (Argentina-Italy) for Research in Clinical and Applied Cryobiology (CAIC), National University of Rosario, Rosario, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24237452

Citation

Carnevale, Matías E., et al. "Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non-heart-beating Donor Rats." Artificial Organs, vol. 37, no. 11, 2013, pp. 985-91.
Carnevale ME, Balaban CL, Guibert EE, et al. Hypothermic machine perfusion versus cold storage in the rescuing of livers from non-heart-beating donor rats. Artif Organs. 2013;37(11):985-91.
Carnevale, M. E., Balaban, C. L., Guibert, E. E., Bottai, H., & Rodriguez, J. V. (2013). Hypothermic machine perfusion versus cold storage in the rescuing of livers from non-heart-beating donor rats. Artificial Organs, 37(11), pp. 985-91. doi:10.1111/aor.12235.
Carnevale ME, et al. Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non-heart-beating Donor Rats. Artif Organs. 2013;37(11):985-91. PubMed PMID: 24237452.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypothermic machine perfusion versus cold storage in the rescuing of livers from non-heart-beating donor rats. AU - Carnevale,Matías E, AU - Balaban,Cecilia L, AU - Guibert,Edgardo E, AU - Bottai,Hebe, AU - Rodriguez,Joaquin V, Y1 - 2013/11/14/ PY - 2013/11/19/entrez PY - 2013/11/19/pubmed PY - 2014/7/16/medline KW - Cold storage KW - Hypothermic machine perfusion KW - Liver KW - Non-heart-beating donors SP - 985 EP - 91 JF - Artificial organs JO - Artif Organs VL - 37 IS - 11 N2 - The aim of this work was to compare the efficiency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs-Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS. SN - 1525-1594 UR - https://www.unboundmedicine.com/medline/citation/24237452/Hypothermic_machine_perfusion_versus_cold_storage_in_the_rescuing_of_livers_from_non_heart_beating_donor_rats_ L2 - https://doi.org/10.1111/aor.12235 DB - PRIME DP - Unbound Medicine ER -