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Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli.
Mol Gen Genet 1986; 203(1):143-9MG

Abstract

The concentration of plasmid pBR322, of its replication inhibitor, RNAI, and preprimer, RNAII, were observed in E. coli as functions of the bacterial growth rate. At growth rates between 0.6 and 2.5 doubling/h, the copy number (number of plasmids per genome equivalent of chromosomal DNA) decreased from 32 to 15, the number of plasmids per cell increased from 39 to 55, and the plasmid concentration decreased from 4.6 to 1.1 X 10(10) plasmids per OD460 unit of cell mass. The concentrations of RNAI and RNAII also decreased with increasing growth rate, but differently, such that their ratio, RNAI/RNAII, increased. In glycerol minimal medium both RNAI and RNAII had the same halflife, 0.55 min, and were synthesized at a ratio of about 3 RNAI transcripts per every RNAII transcript. These results were interpreted on the basis of the negative control model and suggest that the activities of the RNAI and RNAII promoters, and the efficiency with which RNAI inhibits plasmid replication, are controlled by the growth rate.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2423846

Citation

Lin-Chao, S, and H Bremer. "Effect of the Bacterial Growth Rate On Replication Control of Plasmid pBR322 in Escherichia Coli." Molecular & General Genetics : MGG, vol. 203, no. 1, 1986, pp. 143-9.
Lin-Chao S, Bremer H. Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli. Mol Gen Genet. 1986;203(1):143-9.
Lin-Chao, S., & Bremer, H. (1986). Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli. Molecular & General Genetics : MGG, 203(1), pp. 143-9.
Lin-Chao S, Bremer H. Effect of the Bacterial Growth Rate On Replication Control of Plasmid pBR322 in Escherichia Coli. Mol Gen Genet. 1986;203(1):143-9. PubMed PMID: 2423846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of the bacterial growth rate on replication control of plasmid pBR322 in Escherichia coli. AU - Lin-Chao,S, AU - Bremer,H, PY - 1986/4/1/pubmed PY - 1986/4/1/medline PY - 1986/4/1/entrez SP - 143 EP - 9 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 203 IS - 1 N2 - The concentration of plasmid pBR322, of its replication inhibitor, RNAI, and preprimer, RNAII, were observed in E. coli as functions of the bacterial growth rate. At growth rates between 0.6 and 2.5 doubling/h, the copy number (number of plasmids per genome equivalent of chromosomal DNA) decreased from 32 to 15, the number of plasmids per cell increased from 39 to 55, and the plasmid concentration decreased from 4.6 to 1.1 X 10(10) plasmids per OD460 unit of cell mass. The concentrations of RNAI and RNAII also decreased with increasing growth rate, but differently, such that their ratio, RNAI/RNAII, increased. In glycerol minimal medium both RNAI and RNAII had the same halflife, 0.55 min, and were synthesized at a ratio of about 3 RNAI transcripts per every RNAII transcript. These results were interpreted on the basis of the negative control model and suggest that the activities of the RNAI and RNAII promoters, and the efficiency with which RNAI inhibits plasmid replication, are controlled by the growth rate. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/2423846/Effect_of_the_bacterial_growth_rate_on_replication_control_of_plasmid_pBR322_in_Escherichia_coli_ L2 - https://www.lens.org/lens/search?q=citation_id:2423846 DB - PRIME DP - Unbound Medicine ER -