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Effect of relA function on the replication of plasmid pBR322 in Escherichia coli.
Mol Gen Genet 1986; 203(1):150-3MG

Abstract

Replication of the plasmid pBR322, and the accumulation and life time of its primer transcript, RNAII, and replication inhibitor, RNAI, were measured in an isogenic relA+/relA pair of E. coli strains during exponential growth, or following amino acid starvation, or during treatment with chloramphenicol. (1) The synthesis rates of RNAI and RNAII decreased during inhibition of protein synthesis in either strain, i.e. their promoters are not under stringent control; (2) during amino acid starvation, RNAI and RNAII lifetimes increased in complex, rel-dependent patterns; (3) the changes in RNAI and RNAII synthesis and accumulation had no immediate effect on the rate of plasmid replication; (4) continued plasmid replication requires a protein which is synthesized during amino acid deprivation or treatment with low concentrations of chloramphenicol in relA+, but not in relA bacteria.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2423847

Citation

Lin-Chao, S, and H Bremer. "Effect of relA Function On the Replication of Plasmid pBR322 in Escherichia Coli." Molecular & General Genetics : MGG, vol. 203, no. 1, 1986, pp. 150-3.
Lin-Chao S, Bremer H. Effect of relA function on the replication of plasmid pBR322 in Escherichia coli. Mol Gen Genet. 1986;203(1):150-3.
Lin-Chao, S., & Bremer, H. (1986). Effect of relA function on the replication of plasmid pBR322 in Escherichia coli. Molecular & General Genetics : MGG, 203(1), pp. 150-3.
Lin-Chao S, Bremer H. Effect of relA Function On the Replication of Plasmid pBR322 in Escherichia Coli. Mol Gen Genet. 1986;203(1):150-3. PubMed PMID: 2423847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of relA function on the replication of plasmid pBR322 in Escherichia coli. AU - Lin-Chao,S, AU - Bremer,H, PY - 1986/4/1/pubmed PY - 1986/4/1/medline PY - 1986/4/1/entrez SP - 150 EP - 3 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 203 IS - 1 N2 - Replication of the plasmid pBR322, and the accumulation and life time of its primer transcript, RNAII, and replication inhibitor, RNAI, were measured in an isogenic relA+/relA pair of E. coli strains during exponential growth, or following amino acid starvation, or during treatment with chloramphenicol. (1) The synthesis rates of RNAI and RNAII decreased during inhibition of protein synthesis in either strain, i.e. their promoters are not under stringent control; (2) during amino acid starvation, RNAI and RNAII lifetimes increased in complex, rel-dependent patterns; (3) the changes in RNAI and RNAII synthesis and accumulation had no immediate effect on the rate of plasmid replication; (4) continued plasmid replication requires a protein which is synthesized during amino acid deprivation or treatment with low concentrations of chloramphenicol in relA+, but not in relA bacteria. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/2423847/Effect_of_relA_function_on_the_replication_of_plasmid_pBR322_in_Escherichia_coli_ L2 - https://www.lens.org/lens/search?q=citation_id:2423847 DB - PRIME DP - Unbound Medicine ER -