Tags

Type your tag names separated by a space and hit enter

Cytotoxicity and ROS production of manufactured silver nanoparticles of different sizes in hepatoma and leukemia cells.
J Appl Toxicol. 2014 Apr; 34(4):413-23.JA

Abstract

Silver nanoparticles (AgNPs), which have well-known antimicrobial properties, are extensively used in various medical and general applications. In spite of the widespread use of AgNPs, relatively few studies have been undertaken to determine the cytotoxic effects of AgNPs. The aim of this study was investigate how AgNPs of different sizes (4.7 and 42 nm) interact with two different tumoral human cell lines (hepatoma [HepG2] and leukemia [HL-60]). In addition, glutathione depletion, inhibition of superoxide dismutase (SOD) and reactive oxygen species (ROS) generation were used to evaluate feasible mechanisms by which AgNPs exerted its toxicity. AgNPs of 4.7 nm and 42 nm exhibited a dramatic difference in cytotoxicity. Small AgNPs were much more cytotoxic than large AgNPs. A difference in the cellular response to AgNPs was found. HepG2 cells showed a higher sensitivity to the AgNPs than HL-60. However, the cytotoxicity induced by AgNPs was efficiently prevented by NAC treatment, which suggests that oxidative stress is primarily responsible for the cytotoxicity of AgNPs. Furthermore, cellular antioxidant status was disturbed: AgNPs exposure caused ROS production, glutathione depletion and slight, but not statistically significant inactivation of SOD.

Authors+Show Affiliations

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24243578

Citation

Avalos, Alicia, et al. "Cytotoxicity and ROS Production of Manufactured Silver Nanoparticles of Different Sizes in Hepatoma and Leukemia Cells." Journal of Applied Toxicology : JAT, vol. 34, no. 4, 2014, pp. 413-23.
Avalos A, Haza AI, Mateo D, et al. Cytotoxicity and ROS production of manufactured silver nanoparticles of different sizes in hepatoma and leukemia cells. J Appl Toxicol. 2014;34(4):413-23.
Avalos, A., Haza, A. I., Mateo, D., & Morales, P. (2014). Cytotoxicity and ROS production of manufactured silver nanoparticles of different sizes in hepatoma and leukemia cells. Journal of Applied Toxicology : JAT, 34(4), 413-23. https://doi.org/10.1002/jat.2957
Avalos A, et al. Cytotoxicity and ROS Production of Manufactured Silver Nanoparticles of Different Sizes in Hepatoma and Leukemia Cells. J Appl Toxicol. 2014;34(4):413-23. PubMed PMID: 24243578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxicity and ROS production of manufactured silver nanoparticles of different sizes in hepatoma and leukemia cells. AU - Avalos,Alicia, AU - Haza,Ana Isabel, AU - Mateo,Diego, AU - Morales,Paloma, Y1 - 2013/11/15/ PY - 2013/07/12/received PY - 2013/09/10/revised PY - 2013/09/28/accepted PY - 2013/11/19/entrez PY - 2013/11/19/pubmed PY - 2014/9/10/medline KW - N-acetyl-cisteine KW - cytotoxicity KW - glutathione KW - reactive oxygen species KW - silver nanoparticles KW - superoxide dismutase SP - 413 EP - 23 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 34 IS - 4 N2 - Silver nanoparticles (AgNPs), which have well-known antimicrobial properties, are extensively used in various medical and general applications. In spite of the widespread use of AgNPs, relatively few studies have been undertaken to determine the cytotoxic effects of AgNPs. The aim of this study was investigate how AgNPs of different sizes (4.7 and 42 nm) interact with two different tumoral human cell lines (hepatoma [HepG2] and leukemia [HL-60]). In addition, glutathione depletion, inhibition of superoxide dismutase (SOD) and reactive oxygen species (ROS) generation were used to evaluate feasible mechanisms by which AgNPs exerted its toxicity. AgNPs of 4.7 nm and 42 nm exhibited a dramatic difference in cytotoxicity. Small AgNPs were much more cytotoxic than large AgNPs. A difference in the cellular response to AgNPs was found. HepG2 cells showed a higher sensitivity to the AgNPs than HL-60. However, the cytotoxicity induced by AgNPs was efficiently prevented by NAC treatment, which suggests that oxidative stress is primarily responsible for the cytotoxicity of AgNPs. Furthermore, cellular antioxidant status was disturbed: AgNPs exposure caused ROS production, glutathione depletion and slight, but not statistically significant inactivation of SOD. SN - 1099-1263 UR - https://www.unboundmedicine.com/medline/citation/24243578/Cytotoxicity_and_ROS_production_of_manufactured_silver_nanoparticles_of_different_sizes_in_hepatoma_and_leukemia_cells_ L2 - https://doi.org/10.1002/jat.2957 DB - PRIME DP - Unbound Medicine ER -