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Role of interferon-inducible protein 202 (p202) in the regulation of adipogenesis in mouse adipose-derived stem cells.
Mol Cell Endocrinol. 2014 Feb 15; 382(2):814-24.MC

Abstract

The interferon-inducible protein 202 (p202) has emerged as a key regulator of cell proliferation and differentiation. To explore the role of p202 in adipocyte differentiation, p202 mRNA and protein levels in differentiating mouse adipose-derived stem cells (mASCs) were examined, and were found to continuously increase during mASC adipogenesis. The nuclear and cytoplasmic distribution of p202 in the differentiation process was also determined. In addition, suppression and overexpression of p202 impaired and enhanced the differentiation process, respectively. Further, results of co-immunoprecipitation and co-immunofluorescence showed the interaction and intracellular co-localization of p202 with C/EBPβ, C/EBPα, and PPARγ at intermediate and/or late differentiation stages. Knockdown of p202 interfered with the elevated expression of C/EBPβ, C/EBPα, and PPARγ. In conclusion, the temporal and spatial profiles of p202 and the observed manner in which p202 affected the expression of these transcription factors provided evidence that p202 plays a role during mASC adipogenesis.

Authors+Show Affiliations

College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China. Electronic address: hfli1228@163.com.College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24246779

Citation

Li, Haifang, et al. "Role of Interferon-inducible Protein 202 (p202) in the Regulation of Adipogenesis in Mouse Adipose-derived Stem Cells." Molecular and Cellular Endocrinology, vol. 382, no. 2, 2014, pp. 814-24.
Li H, Liu F, Guo H, et al. Role of interferon-inducible protein 202 (p202) in the regulation of adipogenesis in mouse adipose-derived stem cells. Mol Cell Endocrinol. 2014;382(2):814-24.
Li, H., Liu, F., Guo, H., Zhu, Z., & Jiao, Y. (2014). Role of interferon-inducible protein 202 (p202) in the regulation of adipogenesis in mouse adipose-derived stem cells. Molecular and Cellular Endocrinology, 382(2), 814-24. https://doi.org/10.1016/j.mce.2013.11.006
Li H, et al. Role of Interferon-inducible Protein 202 (p202) in the Regulation of Adipogenesis in Mouse Adipose-derived Stem Cells. Mol Cell Endocrinol. 2014 Feb 15;382(2):814-24. PubMed PMID: 24246779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of interferon-inducible protein 202 (p202) in the regulation of adipogenesis in mouse adipose-derived stem cells. AU - Li,Haifang, AU - Liu,Feihan, AU - Guo,Hengjun, AU - Zhu,Zhiqian, AU - Jiao,Yang, Y1 - 2013/11/15/ PY - 2013/05/08/received PY - 2013/11/06/revised PY - 2013/11/08/accepted PY - 2013/11/20/entrez PY - 2013/11/20/pubmed PY - 2014/8/19/medline KW - Adipogenesis KW - C/EBPs KW - Interferon-inducible protein 202 (p202) KW - Mouse adipose-derived stem cells (mASCs) KW - PPARγ SP - 814 EP - 24 JF - Molecular and cellular endocrinology JO - Mol Cell Endocrinol VL - 382 IS - 2 N2 - The interferon-inducible protein 202 (p202) has emerged as a key regulator of cell proliferation and differentiation. To explore the role of p202 in adipocyte differentiation, p202 mRNA and protein levels in differentiating mouse adipose-derived stem cells (mASCs) were examined, and were found to continuously increase during mASC adipogenesis. The nuclear and cytoplasmic distribution of p202 in the differentiation process was also determined. In addition, suppression and overexpression of p202 impaired and enhanced the differentiation process, respectively. Further, results of co-immunoprecipitation and co-immunofluorescence showed the interaction and intracellular co-localization of p202 with C/EBPβ, C/EBPα, and PPARγ at intermediate and/or late differentiation stages. Knockdown of p202 interfered with the elevated expression of C/EBPβ, C/EBPα, and PPARγ. In conclusion, the temporal and spatial profiles of p202 and the observed manner in which p202 affected the expression of these transcription factors provided evidence that p202 plays a role during mASC adipogenesis. SN - 1872-8057 UR - https://www.unboundmedicine.com/medline/citation/24246779/Role_of_interferon_inducible_protein_202__p202__in_the_regulation_of_adipogenesis_in_mouse_adipose_derived_stem_cells_ DB - PRIME DP - Unbound Medicine ER -