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Selenite protects Caenorhabditis elegans from oxidative stress via DAF-16 and TRXR-1.
Mol Nutr Food Res 2014; 58(4):863-74MN

Abstract

SCOPE

Selenium is an essential micronutrient. In the present study, trace amount of selenite (0.01 μM) was evaluated for oxidative stress resistance and potential associated factors in Caenorhabditis elegans.

METHODS AND RESULTS

Selenite-treated C. elegans showed an increased survival under oxidative stress and thermal stress compared to untreated controls. Further studies demonstrated that the significant stress resistance of selenite on C. elegans could be attributed to its in vivo free radical-scavenging ability. We also found that the oxidative and thermal stress resistance phenotypes by selenite were absent from the forkhead transcription factor daf-16 mutant worms. Moreover, selenite influenced the subcellular distribution of DAF-16 in C. elegans. Furthermore, selenite increased mRNA levels of stress-resistance-related proteins, including superoxide dismutase-3 and heat shock protein-16.2. Additionally, selenite (0.01 μM) upregulated expressions of transgenic C. elegans carrying sod-3::green fluorescent protein (GFP) and hsp-16.2::GFP, whereas this effect was abolished by feeding daf-16 RNA interference in C. elegans. Finally, unlike the wild-type N2 worms, the oxidative stress resistance phenotypes by selenite were both absent from the C. elegans selenoprotein trxr-1 mutant worms and trxr-1 mutants feeding with daf-16 RNA interference.

CONCLUSION

These findings suggest that the antioxidant effects of selenite in C. elegans are mediated via DAF-16 and TRXR-1.

Authors+Show Affiliations

Department of Bioenvironmental Systems Engineering, National Taiwan University, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24254253

Citation

Li, Wen-Hsuan, et al. "Selenite Protects Caenorhabditis Elegans From Oxidative Stress Via DAF-16 and TRXR-1." Molecular Nutrition & Food Research, vol. 58, no. 4, 2014, pp. 863-74.
Li WH, Shi YC, Chang CH, et al. Selenite protects Caenorhabditis elegans from oxidative stress via DAF-16 and TRXR-1. Mol Nutr Food Res. 2014;58(4):863-74.
Li, W. H., Shi, Y. C., Chang, C. H., Huang, C. W., & Hsiu-Chuan Liao, V. (2014). Selenite protects Caenorhabditis elegans from oxidative stress via DAF-16 and TRXR-1. Molecular Nutrition & Food Research, 58(4), pp. 863-74. doi:10.1002/mnfr.201300404.
Li WH, et al. Selenite Protects Caenorhabditis Elegans From Oxidative Stress Via DAF-16 and TRXR-1. Mol Nutr Food Res. 2014;58(4):863-74. PubMed PMID: 24254253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenite protects Caenorhabditis elegans from oxidative stress via DAF-16 and TRXR-1. AU - Li,Wen-Hsuan, AU - Shi,Yeu-Ching, AU - Chang,Chun-Han, AU - Huang,Chi-Wei, AU - Hsiu-Chuan Liao,Vivian, Y1 - 2013/11/20/ PY - 2013/06/03/received PY - 2013/08/20/revised PY - 2013/09/05/accepted PY - 2013/11/21/entrez PY - 2013/11/21/pubmed PY - 2015/11/3/medline KW - Caenorhabditis elegans KW - DAF-16 KW - Oxidative stress KW - Selenium KW - TRXR-1 SP - 863 EP - 74 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 58 IS - 4 N2 - SCOPE: Selenium is an essential micronutrient. In the present study, trace amount of selenite (0.01 μM) was evaluated for oxidative stress resistance and potential associated factors in Caenorhabditis elegans. METHODS AND RESULTS: Selenite-treated C. elegans showed an increased survival under oxidative stress and thermal stress compared to untreated controls. Further studies demonstrated that the significant stress resistance of selenite on C. elegans could be attributed to its in vivo free radical-scavenging ability. We also found that the oxidative and thermal stress resistance phenotypes by selenite were absent from the forkhead transcription factor daf-16 mutant worms. Moreover, selenite influenced the subcellular distribution of DAF-16 in C. elegans. Furthermore, selenite increased mRNA levels of stress-resistance-related proteins, including superoxide dismutase-3 and heat shock protein-16.2. Additionally, selenite (0.01 μM) upregulated expressions of transgenic C. elegans carrying sod-3::green fluorescent protein (GFP) and hsp-16.2::GFP, whereas this effect was abolished by feeding daf-16 RNA interference in C. elegans. Finally, unlike the wild-type N2 worms, the oxidative stress resistance phenotypes by selenite were both absent from the C. elegans selenoprotein trxr-1 mutant worms and trxr-1 mutants feeding with daf-16 RNA interference. CONCLUSION: These findings suggest that the antioxidant effects of selenite in C. elegans are mediated via DAF-16 and TRXR-1. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/24254253/Selenite_protects_Caenorhabditis_elegans_from_oxidative_stress_via_DAF_16_and_TRXR_1_ L2 - https://doi.org/10.1002/mnfr.201300404 DB - PRIME DP - Unbound Medicine ER -