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Esophageal intraepithelial invasion of Helicobacter pylori correlates with atypical hyperplasia.
Int J Cancer. 2014 Jun 01; 134(11):2626-32.IJ

Abstract

Helicobacter pylori (H. pylori), a common pathogen residing in the gastrointestinal tract, has been well characterized in stomach cancer,while its correlation with esophageal cancer remains poorly understood. In this study, we aim to assess the relationship between esophageal intraepithelial H. pylori invasion and inflammation as well as atypical hyperplasia in esophageal squamous epithelial tissues. Esophageal squamous cell carcinoma (ESCC) tissue samples from 196 individuals from both southern and northern esophageal carcinoma high-risk areas in China were examined (125 from northern high-risk areas, 71 from southern high-risk area), while additional 30 samples were collected adjacent to the esophageal squamous cell carcinoma (A-ESCC). H. pylori infection was identified by Giemsa staining, immuno-histochemical staining, and H. pylori 16S rRNA-based PCR. A significant increase of H. pylori infection was found in tumor tissues (including ESCC and A-ESCC samples) compared to that of non-tumor tissues (p < 0.05). The positive rate of H. pylori 16S rRNA in ESCC, A-ESCC, and normal groups were 62.5, 74.1, and 26.7%, respectively. The PCR results showed that the positive incidence of the H. pylori virulence factor CagA gene in tumor (ESCC and A-ESCC) and normal groups was 54.9 and 20%, respectively (p < 0.05). To explore the possible causes of CagA+ H. pylori infection leading to carcinogenesis, we found that CagA+ H. pylori filtrate induced DNA strand breaks in esophageal epithelial NE3 cells, suggesting that H. pylori infection may be an original cause leading to atypical hyperplasia of esophageal squamous epithelial tissues and contributed to pathological carcinogenesis of ESCC.

Authors+Show Affiliations

Key Immunopathology Laboratory of Guangdong Province, Institute of Clinical Pathology & Department of Pathology, Shantou University Medical College, Shantou, Guangdong, China; Shaanxi Provincial People's Hospital, The Third Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24254881

Citation

Li, Wen-sheng, et al. "Esophageal Intraepithelial Invasion of Helicobacter Pylori Correlates With Atypical Hyperplasia." International Journal of Cancer, vol. 134, no. 11, 2014, pp. 2626-32.
Li WS, Tian DP, Guan XY, et al. Esophageal intraepithelial invasion of Helicobacter pylori correlates with atypical hyperplasia. Int J Cancer. 2014;134(11):2626-32.
Li, W. S., Tian, D. P., Guan, X. Y., Yun, H., Wang, H. T., Xiao, Y., Bi, C., Ying, S., & Su, M. (2014). Esophageal intraepithelial invasion of Helicobacter pylori correlates with atypical hyperplasia. International Journal of Cancer, 134(11), 2626-32. https://doi.org/10.1002/ijc.28588
Li WS, et al. Esophageal Intraepithelial Invasion of Helicobacter Pylori Correlates With Atypical Hyperplasia. Int J Cancer. 2014 Jun 1;134(11):2626-32. PubMed PMID: 24254881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Esophageal intraepithelial invasion of Helicobacter pylori correlates with atypical hyperplasia. AU - Li,Wen-sheng, AU - Tian,Dong-ping, AU - Guan,Xiao-ying, AU - Yun,Hailong, AU - Wang,Hong-tao, AU - Xiao,Yinping, AU - Bi,Chao, AU - Ying,Songmin, AU - Su,Min, Y1 - 2013/11/20/ PY - 2013/07/25/received PY - 2013/10/21/revised PY - 2013/10/23/accepted PY - 2013/11/21/entrez PY - 2013/11/21/pubmed PY - 2014/5/23/medline KW - CagA KW - DNA damage response KW - Helicobacter pylori KW - esophageal squamous cell carcinoma KW - genomic instability SP - 2626 EP - 32 JF - International journal of cancer JO - Int J Cancer VL - 134 IS - 11 N2 - Helicobacter pylori (H. pylori), a common pathogen residing in the gastrointestinal tract, has been well characterized in stomach cancer,while its correlation with esophageal cancer remains poorly understood. In this study, we aim to assess the relationship between esophageal intraepithelial H. pylori invasion and inflammation as well as atypical hyperplasia in esophageal squamous epithelial tissues. Esophageal squamous cell carcinoma (ESCC) tissue samples from 196 individuals from both southern and northern esophageal carcinoma high-risk areas in China were examined (125 from northern high-risk areas, 71 from southern high-risk area), while additional 30 samples were collected adjacent to the esophageal squamous cell carcinoma (A-ESCC). H. pylori infection was identified by Giemsa staining, immuno-histochemical staining, and H. pylori 16S rRNA-based PCR. A significant increase of H. pylori infection was found in tumor tissues (including ESCC and A-ESCC samples) compared to that of non-tumor tissues (p < 0.05). The positive rate of H. pylori 16S rRNA in ESCC, A-ESCC, and normal groups were 62.5, 74.1, and 26.7%, respectively. The PCR results showed that the positive incidence of the H. pylori virulence factor CagA gene in tumor (ESCC and A-ESCC) and normal groups was 54.9 and 20%, respectively (p < 0.05). To explore the possible causes of CagA+ H. pylori infection leading to carcinogenesis, we found that CagA+ H. pylori filtrate induced DNA strand breaks in esophageal epithelial NE3 cells, suggesting that H. pylori infection may be an original cause leading to atypical hyperplasia of esophageal squamous epithelial tissues and contributed to pathological carcinogenesis of ESCC. SN - 1097-0215 UR - https://www.unboundmedicine.com/medline/citation/24254881/Esophageal_intraepithelial_invasion_of_Helicobacter_pylori_correlates_with_atypical_hyperplasia_ L2 - https://doi.org/10.1002/ijc.28588 DB - PRIME DP - Unbound Medicine ER -