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The LOV protein of Xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker.
PLoS One. 2013; 8(11):e80930.Plos

Abstract

Pathogens interaction with a host plant starts a set of immune responses that result in complex changes in gene expression and plant physiology. Light is an important modulator of plant defense response and recent studies have evidenced the novel influence of this environmental stimulus in the virulence of several bacterial pathogens. Xanthomonas citri subsp. citri is the bacterium responsible for citrus canker disease, which affects most citrus cultivars. The ability of this bacterium to colonize host plants is influenced by bacterial blue-light sensing through a LOV-domain protein and disease symptoms are considerably altered upon deletion of this protein. In this work we aimed to unravel the role of this photoreceptor during the bacterial counteraction of plant immune responses leading to citrus canker development. We performed a transcriptomic analysis in Citrus sinensis leaves inoculated with the wild type X. citri subsp. citri and with a mutant strain lacking the LOV protein by a cDNA microarray and evaluated the differentially regulated genes corresponding to specific biological processes. A down-regulation of photosynthesis-related genes (together with a corresponding decrease in photosynthesis rates) was observed upon bacterial infection, this effect being more pronounced in plants infected with the lov-mutant bacterial strain. Infection with this strain was also accompanied with the up-regulation of several secondary metabolism- and defense response-related genes. Moreover, we found that relevant plant physiological alterations triggered by pathogen attack such as cell wall fortification and tissue disruption were amplified during the lov-mutant strain infection. These results suggest the participation of the LOV-domain protein from X. citri subsp. citri in the bacterial counteraction of host plant defense response, contributing in this way to disease development.

Authors+Show Affiliations

Instituto de Biología Molecular y Celular de Rosario (IBR - CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas (FBIOYF - UNR), Rosario, Santa Fe, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24260514

Citation

Kraiselburd, Ivana, et al. "The LOV Protein of Xanthomonas Citri Subsp. Citri Plays a Significant Role in the Counteraction of Plant Immune Responses During Citrus Canker." PloS One, vol. 8, no. 11, 2013, pp. e80930.
Kraiselburd I, Daurelio LD, Tondo ML, et al. The LOV protein of Xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker. PLoS One. 2013;8(11):e80930.
Kraiselburd, I., Daurelio, L. D., Tondo, M. L., Merelo, P., Cortadi, A. A., Talón, M., Tadeo, F. R., & Orellano, E. G. (2013). The LOV protein of Xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker. PloS One, 8(11), e80930. https://doi.org/10.1371/journal.pone.0080930
Kraiselburd I, et al. The LOV Protein of Xanthomonas Citri Subsp. Citri Plays a Significant Role in the Counteraction of Plant Immune Responses During Citrus Canker. PLoS One. 2013;8(11):e80930. PubMed PMID: 24260514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The LOV protein of Xanthomonas citri subsp. citri plays a significant role in the counteraction of plant immune responses during citrus canker. AU - Kraiselburd,Ivana, AU - Daurelio,Lucas D, AU - Tondo,María Laura, AU - Merelo,Paz, AU - Cortadi,Adriana A, AU - Talón,Manuel, AU - Tadeo,Francisco R, AU - Orellano,Elena G, Y1 - 2013/11/15/ PY - 2013/06/25/received PY - 2013/10/07/accepted PY - 2013/11/22/entrez PY - 2013/11/22/pubmed PY - 2014/7/1/medline SP - e80930 EP - e80930 JF - PloS one JO - PLoS One VL - 8 IS - 11 N2 - Pathogens interaction with a host plant starts a set of immune responses that result in complex changes in gene expression and plant physiology. Light is an important modulator of plant defense response and recent studies have evidenced the novel influence of this environmental stimulus in the virulence of several bacterial pathogens. Xanthomonas citri subsp. citri is the bacterium responsible for citrus canker disease, which affects most citrus cultivars. The ability of this bacterium to colonize host plants is influenced by bacterial blue-light sensing through a LOV-domain protein and disease symptoms are considerably altered upon deletion of this protein. In this work we aimed to unravel the role of this photoreceptor during the bacterial counteraction of plant immune responses leading to citrus canker development. We performed a transcriptomic analysis in Citrus sinensis leaves inoculated with the wild type X. citri subsp. citri and with a mutant strain lacking the LOV protein by a cDNA microarray and evaluated the differentially regulated genes corresponding to specific biological processes. A down-regulation of photosynthesis-related genes (together with a corresponding decrease in photosynthesis rates) was observed upon bacterial infection, this effect being more pronounced in plants infected with the lov-mutant bacterial strain. Infection with this strain was also accompanied with the up-regulation of several secondary metabolism- and defense response-related genes. Moreover, we found that relevant plant physiological alterations triggered by pathogen attack such as cell wall fortification and tissue disruption were amplified during the lov-mutant strain infection. These results suggest the participation of the LOV-domain protein from X. citri subsp. citri in the bacterial counteraction of host plant defense response, contributing in this way to disease development. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24260514/The_LOV_protein_of_Xanthomonas_citri_subsp__citri_plays_a_significant_role_in_the_counteraction_of_plant_immune_responses_during_citrus_canker_ L2 - https://dx.plos.org/10.1371/journal.pone.0080930 DB - PRIME DP - Unbound Medicine ER -