Tags

Type your tag names separated by a space and hit enter

Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries.
PLoS One 2013; 8(11):e81297Plos

Abstract

Bee propolis is a mixture of plant resins and bee secretions. While bioactivity of honeybee propolis has been reported previously, information is limited on propolis from Australian stingless bees (Tetragonula carbonaria). The aim of this study was to investigate possible vasomodulatory effects of propolis in KCl-precontracted porcine coronary arteries using an ex vivo tissue bath assay. Polar extracts of propolis produced a dose-dependent relaxant response (EC50=44.7±7.0 μg/ml), which was unaffected by endothelial denudation, suggesting a direct effect on smooth muscle. Propolis markedly attenuated a contractile response to Ca(2+) in vessels that were depolarised with 60 mM KCl, in Ca(2+)-free Krebs solution. Propolis (160 µg/ml) reduced vascular tone in KCl pre-contracted vessels to near-baseline levels over 90 min, and this effect was partially reversible with 6 h washout. Some loss in membrane integrity, but no loss in mitochondrial function was detected after 90 min exposure of human cultured umbilical vein endothelial cells to 160 µg/ml propolis. We conclude that Australian stingless bee (T. carbonaria) propolis relaxes porcine coronary artery in an endothelial-independent manner that involves inhibition of voltage-gated Ca(2+) channels. This effect is partially and slowly reversible upon washout. Further studies are required to determine the therapeutic potential of Australian stingless bee propolis for conditions in which vascular supply is compromised.

Authors+Show Affiliations

Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24260567

Citation

Massaro, Flavia C., et al. "Effect of Australian Propolis From Stingless Bees (Tetragonula Carbonaria) On Pre-contracted Human and Porcine Isolated Arteries." PloS One, vol. 8, no. 11, 2013, pp. e81297.
Massaro FC, Brooks PR, Wallace HM, et al. Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries. PLoS ONE. 2013;8(11):e81297.
Massaro, F. C., Brooks, P. R., Wallace, H. M., Nsengiyumva, V., Narokai, L., & Russell, F. D. (2013). Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries. PloS One, 8(11), pp. e81297. doi:10.1371/journal.pone.0081297.
Massaro FC, et al. Effect of Australian Propolis From Stingless Bees (Tetragonula Carbonaria) On Pre-contracted Human and Porcine Isolated Arteries. PLoS ONE. 2013;8(11):e81297. PubMed PMID: 24260567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Australian propolis from stingless bees (Tetragonula carbonaria) on pre-contracted human and porcine isolated arteries. AU - Massaro,Flavia C, AU - Brooks,Peter R, AU - Wallace,Helen M, AU - Nsengiyumva,Vianne, AU - Narokai,Lorraine, AU - Russell,Fraser D, Y1 - 2013/11/15/ PY - 2012/07/05/received PY - 2013/10/22/accepted PY - 2013/11/22/entrez PY - 2013/11/22/pubmed PY - 2014/7/1/medline SP - e81297 EP - e81297 JF - PloS one JO - PLoS ONE VL - 8 IS - 11 N2 - Bee propolis is a mixture of plant resins and bee secretions. While bioactivity of honeybee propolis has been reported previously, information is limited on propolis from Australian stingless bees (Tetragonula carbonaria). The aim of this study was to investigate possible vasomodulatory effects of propolis in KCl-precontracted porcine coronary arteries using an ex vivo tissue bath assay. Polar extracts of propolis produced a dose-dependent relaxant response (EC50=44.7±7.0 μg/ml), which was unaffected by endothelial denudation, suggesting a direct effect on smooth muscle. Propolis markedly attenuated a contractile response to Ca(2+) in vessels that were depolarised with 60 mM KCl, in Ca(2+)-free Krebs solution. Propolis (160 µg/ml) reduced vascular tone in KCl pre-contracted vessels to near-baseline levels over 90 min, and this effect was partially reversible with 6 h washout. Some loss in membrane integrity, but no loss in mitochondrial function was detected after 90 min exposure of human cultured umbilical vein endothelial cells to 160 µg/ml propolis. We conclude that Australian stingless bee (T. carbonaria) propolis relaxes porcine coronary artery in an endothelial-independent manner that involves inhibition of voltage-gated Ca(2+) channels. This effect is partially and slowly reversible upon washout. Further studies are required to determine the therapeutic potential of Australian stingless bee propolis for conditions in which vascular supply is compromised. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24260567/Effect_of_Australian_propolis_from_stingless_bees__Tetragonula_carbonaria__on_pre_contracted_human_and_porcine_isolated_arteries_ L2 - http://dx.plos.org/10.1371/journal.pone.0081297 DB - PRIME DP - Unbound Medicine ER -