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Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve.
J Ethnopharmacol. 2014; 151(1):445-51.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The roots of Nauclea latifolia Smith (Rubiaceae) popularly known as "koumkouma" is used in traditional Cameroonian medicine as neuropathic pain remedy and for the treatment of headache, inflammatory pain and convulsion. This study was conducted to evaluate the antinociceptive effects of the alkaloid fraction isolated from Nauclea latifolia in neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in rat.

MATERIALS AND METHODS

Bioactive-guided fractionation of the root extracts of Nauclea latifolia using the Von Frey in a rat model of neuropathic pain (Benett model), afforded a potent anti-hyperalgesic fraction IV. Further fractionation of this fraction was performed by high-performance liquid chromatography (HPLC), yielded eight sub-fractions (F1-F8) which were tested for antinociceptive effects. The alkaloid fraction (F3) collected by HPLC, exhibited potent antinociceptive effects, and the anti-allodynic and anti-hyperalgesic effects of this fraction (8, 16, 40 and 80 mg/kg) were determined using the von Frey and acetone tests respectively in a rat model of neuropathic pain. Rota-rod performance and catalepsy tests were used for the assessment of motor coordination.

RESULTS

The alkaloid fraction (80 mg/kg) administered intraperitoneally induced a completely decreased hyperalgesia 90 min post-dosing. In the acetone test, the Nauclea latifolia fraction at 80mg/kg showed its maximal anti-allodynic effects 120 min post-injection. The areas under the curve (AUC) of the anti-allodynic or anti-hyperalgesic effects produced by the alkaloid fraction at 80 mg/kg were significantly (p<0.001) greater than the AUC of effects produced by vehicle in CCI rats. The alkaloid fraction did not exhibit any significant effects on the spontaneous locomotor activity of the mice in rota-rod performance and no sign of catalepsy was observed.

CONCLUSION

The analysis of the effects, expressed as the time course of AUC, supports the traditional use of Nauclea latifolia in neuropathic pain therapy. The pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for this anti-hyperalgesic and anti-allodynic action and also to identify the active substances present in the roots extracts of Nauclea latifolia.

Authors+Show Affiliations

Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon; Unité Inserm U836, Grenoble Institute of Neuroscience, Chemin Fortuné Ferrini, Site santé de la Tronche, P.O. Box 170, 38042 Cedex 9 Grenoble, France; Université Joseph Fourier, Grenoble, France. Electronic address: taiwe_sotoing@yahoo.fr.Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454 Ngaoundéré, Cameroon.Department of Chemistry, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon.Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.Department of Chemistry, Higher Teachers' Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon.Unité Inserm U836, Grenoble Institute of Neuroscience, Chemin Fortuné Ferrini, Site santé de la Tronche, P.O. Box 170, 38042 Cedex 9 Grenoble, France; Université Joseph Fourier, Grenoble, France.Unité Inserm U836, Grenoble Institute of Neuroscience, Chemin Fortuné Ferrini, Site santé de la Tronche, P.O. Box 170, 38042 Cedex 9 Grenoble, France; Université Joseph Fourier, Grenoble, France.Université Joseph Fourier, Grenoble, France; Unité CNRS 5063, Department of Medicinal Chemistry, P.O. Box 53, 38041 Grenoble Cedex 9, France; Smartox Biotechnologies, Floralis, Biopolis, 5 Avenue du Grand Sablon, 38700 La Tronche, France.Unité Inserm U836, Grenoble Institute of Neuroscience, Chemin Fortuné Ferrini, Site santé de la Tronche, P.O. Box 170, 38042 Cedex 9 Grenoble, France; Université Joseph Fourier, Grenoble, France; Smartox Biotechnologies, Floralis, Biopolis, 5 Avenue du Grand Sablon, 38700 La Tronche, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24263011

Citation

Taïwe, Germain Sotoing, et al. "Nauclea Latifolia Smith (Rubiaceae) Exerts Antinociceptive Effects in Neuropathic Pain Induced By Chronic Constriction Injury of the Sciatic Nerve." Journal of Ethnopharmacology, vol. 151, no. 1, 2014, pp. 445-51.
Taïwe GS, Bum EN, Talla E, et al. Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve. J Ethnopharmacol. 2014;151(1):445-51.
Taïwe, G. S., Bum, E. N., Talla, E., Dimo, T., Dawe, A., Sinniger, V., Bonaz, B., Boumendjel, A., & De Waard, M. (2014). Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve. Journal of Ethnopharmacology, 151(1), 445-51. https://doi.org/10.1016/j.jep.2013.10.068
Taïwe GS, et al. Nauclea Latifolia Smith (Rubiaceae) Exerts Antinociceptive Effects in Neuropathic Pain Induced By Chronic Constriction Injury of the Sciatic Nerve. J Ethnopharmacol. 2014;151(1):445-51. PubMed PMID: 24263011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nauclea latifolia Smith (Rubiaceae) exerts antinociceptive effects in neuropathic pain induced by chronic constriction injury of the sciatic nerve. AU - Taïwe,Germain Sotoing, AU - Bum,Elisabeth Ngo, AU - Talla,Emmanuel, AU - Dimo,Théophile, AU - Dawe,Amadou, AU - Sinniger,Valérie, AU - Bonaz,Bruno, AU - Boumendjel,Ahcène, AU - De Waard,Michel, Y1 - 2013/11/18/ PY - 2013/08/03/received PY - 2013/10/26/revised PY - 2013/10/27/accepted PY - 2013/11/23/entrez PY - 2013/11/23/pubmed PY - 2014/9/10/medline KW - 3-(4,5 dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide KW - ANOVA KW - ATCC KW - AUC KW - American type culture collection KW - Anti-allodynic KW - Anti-hyperalgesic KW - CCI KW - CHO KW - Chinese hamster ovary KW - EC(50) KW - F(1) KW - HPLC KW - LD(50) KW - MTT KW - N(ω)-l-nitro-arginine methyl ester KW - NIH KW - National Institutes of Health KW - Nauclea latifolia KW - Neuropthic pain KW - Traditional medicine. KW - analysis of variance KW - areas under the curve KW - chronic constriction injury KW - fractions KW - high-performance liquid chromatography KW - l-NAME KW - median lethal dose KW - the 50% effective concentration SP - 445 EP - 51 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 151 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The roots of Nauclea latifolia Smith (Rubiaceae) popularly known as "koumkouma" is used in traditional Cameroonian medicine as neuropathic pain remedy and for the treatment of headache, inflammatory pain and convulsion. This study was conducted to evaluate the antinociceptive effects of the alkaloid fraction isolated from Nauclea latifolia in neuropathic pain induced by chronic constriction injury (CCI) of the sciatic nerve in rat. MATERIALS AND METHODS: Bioactive-guided fractionation of the root extracts of Nauclea latifolia using the Von Frey in a rat model of neuropathic pain (Benett model), afforded a potent anti-hyperalgesic fraction IV. Further fractionation of this fraction was performed by high-performance liquid chromatography (HPLC), yielded eight sub-fractions (F1-F8) which were tested for antinociceptive effects. The alkaloid fraction (F3) collected by HPLC, exhibited potent antinociceptive effects, and the anti-allodynic and anti-hyperalgesic effects of this fraction (8, 16, 40 and 80 mg/kg) were determined using the von Frey and acetone tests respectively in a rat model of neuropathic pain. Rota-rod performance and catalepsy tests were used for the assessment of motor coordination. RESULTS: The alkaloid fraction (80 mg/kg) administered intraperitoneally induced a completely decreased hyperalgesia 90 min post-dosing. In the acetone test, the Nauclea latifolia fraction at 80mg/kg showed its maximal anti-allodynic effects 120 min post-injection. The areas under the curve (AUC) of the anti-allodynic or anti-hyperalgesic effects produced by the alkaloid fraction at 80 mg/kg were significantly (p<0.001) greater than the AUC of effects produced by vehicle in CCI rats. The alkaloid fraction did not exhibit any significant effects on the spontaneous locomotor activity of the mice in rota-rod performance and no sign of catalepsy was observed. CONCLUSION: The analysis of the effects, expressed as the time course of AUC, supports the traditional use of Nauclea latifolia in neuropathic pain therapy. The pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for this anti-hyperalgesic and anti-allodynic action and also to identify the active substances present in the roots extracts of Nauclea latifolia. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24263011/Nauclea_latifolia_Smith__Rubiaceae__exerts_antinociceptive_effects_in_neuropathic_pain_induced_by_chronic_constriction_injury_of_the_sciatic_nerve_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00794-0 DB - PRIME DP - Unbound Medicine ER -