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Community composition of the Planctomycetes associated with different macroalgae.
FEMS Microbiol Ecol. 2014 Jun; 88(3):445-56.FM

Abstract

Insights into the diversity of marine natural microbial biofilms, as for example those developing at the surface of marine macroalgae, can be obtained by using molecular techniques based on 16S rRNA genes. We applied denaturing gradient gel electrophoresis (DGGE) with 16S rRNA genes-specific primers for Planctomycetes to compare the communities of these organisms developing on six different macroalgae (Chondrus crispus, Fucus spiralis, Mastocarpus stellatus, Porphyra dioica, Sargassum muticum, and Ulva sp.) sampled in spring 2012 in two rocky beaches in the north of Portugal. Planctomycetes can be one of the dominant organisms found in the epibacterial community of macroalgae, and we wanted to determine the degree of specificity and the spatial variation of these group. Shannon diversity indexes obtained from the comparison of DGGE profiles were similar in all the macroalgae, and in both sites, F. spiralis was the algae presenting lower Planctomycetes diversity, while M. stellatus and P. dioica from Porto showed the highest diversity. The analysis of DGGE profiles, including anosim statistics, indicate the existence of a specific Planctomycetes community associated with the algal host, likely independent of geographical variation. Sequencing of DGGE bands indicated that Planctomycetes communities were highly diverse, and some Operational Taxonomic Units seemed to be specifically associated with each macroalgae.

Authors+Show Affiliations

Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Porto, Portugal; CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Porto, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24266389

Citation

Bondoso, Joana, et al. "Community Composition of the Planctomycetes Associated With Different Macroalgae." FEMS Microbiology Ecology, vol. 88, no. 3, 2014, pp. 445-56.
Bondoso J, Balagué V, Gasol JM, et al. Community composition of the Planctomycetes associated with different macroalgae. FEMS Microbiol Ecol. 2014;88(3):445-56.
Bondoso, J., Balagué, V., Gasol, J. M., & Lage, O. M. (2014). Community composition of the Planctomycetes associated with different macroalgae. FEMS Microbiology Ecology, 88(3), 445-56. https://doi.org/10.1111/1574-6941.12258
Bondoso J, et al. Community Composition of the Planctomycetes Associated With Different Macroalgae. FEMS Microbiol Ecol. 2014;88(3):445-56. PubMed PMID: 24266389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Community composition of the Planctomycetes associated with different macroalgae. AU - Bondoso,Joana, AU - Balagué,Vanessa, AU - Gasol,Josep M, AU - Lage,Olga M, Y1 - 2014/05/12/ PY - 2013/10/09/received PY - 2013/10/29/revised PY - 2013/11/18/accepted PY - 2013/11/26/entrez PY - 2013/11/26/pubmed PY - 2014/9/23/medline KW - Planctomycetes KW - biofilm KW - denaturing gradient gel electrophoresis KW - host specificity KW - macroalgae SP - 445 EP - 56 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 88 IS - 3 N2 - Insights into the diversity of marine natural microbial biofilms, as for example those developing at the surface of marine macroalgae, can be obtained by using molecular techniques based on 16S rRNA genes. We applied denaturing gradient gel electrophoresis (DGGE) with 16S rRNA genes-specific primers for Planctomycetes to compare the communities of these organisms developing on six different macroalgae (Chondrus crispus, Fucus spiralis, Mastocarpus stellatus, Porphyra dioica, Sargassum muticum, and Ulva sp.) sampled in spring 2012 in two rocky beaches in the north of Portugal. Planctomycetes can be one of the dominant organisms found in the epibacterial community of macroalgae, and we wanted to determine the degree of specificity and the spatial variation of these group. Shannon diversity indexes obtained from the comparison of DGGE profiles were similar in all the macroalgae, and in both sites, F. spiralis was the algae presenting lower Planctomycetes diversity, while M. stellatus and P. dioica from Porto showed the highest diversity. The analysis of DGGE profiles, including anosim statistics, indicate the existence of a specific Planctomycetes community associated with the algal host, likely independent of geographical variation. Sequencing of DGGE bands indicated that Planctomycetes communities were highly diverse, and some Operational Taxonomic Units seemed to be specifically associated with each macroalgae. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/24266389/Community_composition_of_the_Planctomycetes_associated_with_different_macroalgae_ DB - PRIME DP - Unbound Medicine ER -