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Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering.

Abstract

In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I composite nanofibrous scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin nanofibrous scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the nanofibrous PCL/gelatin scaffold was modified by collagen type I (0.2-1.5wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin composite scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin scaffold by collagen type I immobilization on the surface of the scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified composite scaffold. FE-SEM analysis of cell-scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified scaffold. Above results suggest that the collagen type I-modified PCL/gelatin scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I composite nanofibrous scaffold might be a potential candidate for wound healing and skin tissue engineering applications.

Authors+Show Affiliations

Department of Polymer & Process Engineering, Indian Institute of Technology Roorkee, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24268275

Citation

Gautam, Sneh, et al. "Surface Modification of Nanofibrous Polycaprolactone/gelatin Composite Scaffold By Collagen Type I Grafting for Skin Tissue Engineering." Materials Science & Engineering. C, Materials for Biological Applications, vol. 34, 2014, pp. 402-9.
Gautam S, Chou CF, Dinda AK, et al. Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Mater Sci Eng C Mater Biol Appl. 2014;34:402-9.
Gautam, S., Chou, C. F., Dinda, A. K., Potdar, P. D., & Mishra, N. C. (2014). Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Materials Science & Engineering. C, Materials for Biological Applications, 34, pp. 402-9. doi:10.1016/j.msec.2013.09.043.
Gautam S, et al. Surface Modification of Nanofibrous Polycaprolactone/gelatin Composite Scaffold By Collagen Type I Grafting for Skin Tissue Engineering. Mater Sci Eng C Mater Biol Appl. 2014 Jan 1;34:402-9. PubMed PMID: 24268275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. AU - Gautam,Sneh, AU - Chou,Chia-Fu, AU - Dinda,Amit K, AU - Potdar,Pravin D, AU - Mishra,Narayan C, Y1 - 2013/10/05/ PY - 2013/05/19/received PY - 2013/08/27/revised PY - 2013/09/28/accepted PY - 2013/11/26/entrez PY - 2013/11/26/pubmed PY - 2014/8/27/medline KW - Collagen type I KW - Electrospinning KW - Gelatin KW - PCL KW - Skin tissue engineering SP - 402 EP - 9 JF - Materials science & engineering. C, Materials for biological applications JO - Mater Sci Eng C Mater Biol Appl VL - 34 N2 - In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I composite nanofibrous scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin nanofibrous scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the nanofibrous PCL/gelatin scaffold was modified by collagen type I (0.2-1.5wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin composite scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin scaffold by collagen type I immobilization on the surface of the scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified composite scaffold. FE-SEM analysis of cell-scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified scaffold. Above results suggest that the collagen type I-modified PCL/gelatin scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I composite nanofibrous scaffold might be a potential candidate for wound healing and skin tissue engineering applications. SN - 1873-0191 UR - https://www.unboundmedicine.com/medline/citation/24268275/Surface_modification_of_nanofibrous_polycaprolactone/gelatin_composite_scaffold_by_collagen_type_I_grafting_for_skin_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-4931(13)00565-1 DB - PRIME DP - Unbound Medicine ER -