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The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats.
J Ethnopharmacol. 2014; 151(1):591-600.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Moutan Cortex (MC, family: Paeonia suffruticosa Andr.) is a well-known traditional herbal medicine that has been shown to hold a protective effect on inflammation in several diseases. However, its anti-inflammatory activity on diabetic nephropathy (DN) has been less reported. The present study was conducted to evaluate the potential attenuation activities of MC on inflammation in AGEs-induced rat mesangial cells dysfunction and high-glucose-fat diet and streptozotocin (STZ)-induced DN rats and explore the possible mechanism underlying its DN effect.

MATERIALS AND METHODS

The inflammation in mesangial cells (HBZY-1) was induced by 200 μg/ml advanced glycation end products (AGEs). DN rats model was established by an administration high-glucose-fat diet and an intraperitoneal injection of STZ (30 mg/kg). Interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) level in cell supernatant and rats serum were detected by appropriate kits. A co-culture system of mesangial cells and macrophages was performed to evaluate the migration of macrophages. Immunohistochemical assay was applied to examine transforming growth factor beta1 (TGF-β1), IL-6, MCP-1 and intercellular adhesion molecule-1 (ICAM-1) expression in kidney tissues of rats. Furthermore, western blot analysis was carried out to examine TGF-β1, IL-6, MCP-1, ICAM-1 and RAGE protein expressions in mesangial cells.

RESULTS

Pretreatment with MC could significantly inhibit AGEs-induced migration of macrophages in the co-culture system of mesangial cell and macrophage. MC could decrease IL-6 and MCP-1 levels in serum of DN rats in a dose-dependent manner. Furthermore, MC also improved the blood glucose, serum creatinine and urine protein levels. Both immunocytochemistry analysis and western blot analysis showed that MC decreased significantly the over-expression of IL-6, MCP-1, TGF-β1, ICAM-1 and RAGE in mesangial cells or kidney tissues. Additionally, the protein expression of proinflammatory cytokine could also be down-regulated by the pretreatment of RAGE-Ab (5 μg/ml).

CONCLUSION

These findings indicated that the extract of MC had an amelioration activity on the inflammation in AGEs-induced mesangial cells dysfunction and high-glucose-fat diet and STZ-induced DN rats. The protective effect might be associated with the intervention of MC via target of RAGE. These findings suggested that MC might be a benefit agent for the prevention and treatment of DN.

Authors+Show Affiliations

Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Department of Pharmaceutics, Jiangsu University, Jiangsu, Zhenjiang 212013, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China. Electronic address: wenmoxiushi@163.com.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Nanjing Institute of Supervision & Testing on Product Quality, Jiangsu, Nanjing 210028, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Nanjing Institute of Supervision & Testing on Product Quality, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China.Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Department of Pharmaceutics, Jiangsu University, Jiangsu, Zhenjiang 212013, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. Electronic address: jxiaobin2005@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24269777

Citation

Zhang, Ming-hua, et al. "The Anti-inflammation Effect of Moutan Cortex On Advanced Glycation End Products-induced Rat Mesangial Cells Dysfunction and High-glucose-fat Diet and Streptozotocin-induced Diabetic Nephropathy Rats." Journal of Ethnopharmacology, vol. 151, no. 1, 2014, pp. 591-600.
Zhang MH, Feng L, Zhu MM, et al. The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats. J Ethnopharmacol. 2014;151(1):591-600.
Zhang, M. H., Feng, L., Zhu, M. M., Gu, J. F., Jiang, J., Cheng, X. D., Ding, S. M., Wu, C., & Jia, X. B. (2014). The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats. Journal of Ethnopharmacology, 151(1), 591-600. https://doi.org/10.1016/j.jep.2013.11.015
Zhang MH, et al. The Anti-inflammation Effect of Moutan Cortex On Advanced Glycation End Products-induced Rat Mesangial Cells Dysfunction and High-glucose-fat Diet and Streptozotocin-induced Diabetic Nephropathy Rats. J Ethnopharmacol. 2014;151(1):591-600. PubMed PMID: 24269777.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats. AU - Zhang,Ming-hua, AU - Feng,Liang, AU - Zhu,Mao-mao, AU - Gu,Jun-fei, AU - Jiang,Jun, AU - Cheng,Xu-dong, AU - Ding,Shu-ming, AU - Wu,Chan, AU - Jia,Xiao-bin, Y1 - 2013/11/21/ PY - 2013/03/14/received PY - 2013/08/15/revised PY - 2013/11/10/accepted PY - 2013/11/26/entrez PY - 2013/11/26/pubmed PY - 2014/9/10/medline KW - AG KW - AGEs KW - BSA KW - DMEM KW - DN KW - Diabetic nephropathy KW - Dulbecco's modified Eagle's medium KW - FBS KW - ICAM-1 KW - IL-6 KW - Inflammation KW - MC KW - MCP-1 KW - Moutan Cortex KW - Moutan cortex KW - OD KW - OS KW - PBS KW - RAGE KW - ROS KW - Rage KW - SD KW - STZ KW - TGF-β1 KW - advanced glycation end products KW - aminoguanidine KW - bovine serum albumin KW - diabetic nephropathy KW - fetal bovine serum KW - intercellular adhesion molecule-1 KW - interleukin-6 KW - monocyte chemoattractant protein-1 KW - optical density KW - oxidative stress KW - phosphate buffered saline KW - reactive oxygen species KW - receptor for AGEs KW - standard deviation KW - streptozotocin KW - transforming growth factor beta1 SP - 591 EP - 600 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 151 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Moutan Cortex (MC, family: Paeonia suffruticosa Andr.) is a well-known traditional herbal medicine that has been shown to hold a protective effect on inflammation in several diseases. However, its anti-inflammatory activity on diabetic nephropathy (DN) has been less reported. The present study was conducted to evaluate the potential attenuation activities of MC on inflammation in AGEs-induced rat mesangial cells dysfunction and high-glucose-fat diet and streptozotocin (STZ)-induced DN rats and explore the possible mechanism underlying its DN effect. MATERIALS AND METHODS: The inflammation in mesangial cells (HBZY-1) was induced by 200 μg/ml advanced glycation end products (AGEs). DN rats model was established by an administration high-glucose-fat diet and an intraperitoneal injection of STZ (30 mg/kg). Interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) level in cell supernatant and rats serum were detected by appropriate kits. A co-culture system of mesangial cells and macrophages was performed to evaluate the migration of macrophages. Immunohistochemical assay was applied to examine transforming growth factor beta1 (TGF-β1), IL-6, MCP-1 and intercellular adhesion molecule-1 (ICAM-1) expression in kidney tissues of rats. Furthermore, western blot analysis was carried out to examine TGF-β1, IL-6, MCP-1, ICAM-1 and RAGE protein expressions in mesangial cells. RESULTS: Pretreatment with MC could significantly inhibit AGEs-induced migration of macrophages in the co-culture system of mesangial cell and macrophage. MC could decrease IL-6 and MCP-1 levels in serum of DN rats in a dose-dependent manner. Furthermore, MC also improved the blood glucose, serum creatinine and urine protein levels. Both immunocytochemistry analysis and western blot analysis showed that MC decreased significantly the over-expression of IL-6, MCP-1, TGF-β1, ICAM-1 and RAGE in mesangial cells or kidney tissues. Additionally, the protein expression of proinflammatory cytokine could also be down-regulated by the pretreatment of RAGE-Ab (5 μg/ml). CONCLUSION: These findings indicated that the extract of MC had an amelioration activity on the inflammation in AGEs-induced mesangial cells dysfunction and high-glucose-fat diet and STZ-induced DN rats. The protective effect might be associated with the intervention of MC via target of RAGE. These findings suggested that MC might be a benefit agent for the prevention and treatment of DN. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24269777/The_anti_inflammation_effect_of_Moutan_Cortex_on_advanced_glycation_end_products_induced_rat_mesangial_cells_dysfunction_and_High_glucose_fat_diet_and_streptozotocin_induced_diabetic_nephropathy_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00809-X DB - PRIME DP - Unbound Medicine ER -