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Validated rapid resolution LC-ESI-MS/MS method for simultaneous determination of five pulchinenosides from Pulsatilla chinensis (Bunge) Regel in rat plasma: application to pharmacokinetics and bioavailability studies.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 30; 942-943:141-50.JC

Abstract

A simple, RRLC-ESI-MS/MS method was developed for the simultaneous determination of five oleanane pulchinenosides (B3, BD, B7, B10, and B11), in rat plasma following solid-phase extraction (SPE). Detection and quantitation were performed by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) mode. The MS/MS transitions of the triterpenoidal saponins: m/z 911.4→603.2, 749.4→471.3, 895.6→733.2, 733.5→455.3, and 579.3→371.1 were monitored for B3, BD, B7 and B10, B11 and internal standard (Forsythin), respectively. The method was validated in plasma samples, showed good linearity over a wide concentration range (r(2)>0.99), and with lower limits of quantification of 1.11 (B3), 0.751 (BD), 0.996 (B7), 0.415 (B10), and 0.332 (B11)ng/mL. The intra- and inter-day assay variability was less than 15% for all analytes. The mean extraction recoveries of analytes and IS from rats plasma were all more than 70.0%. The validation results demonstrate that this method is robust and specific. The validated method was successfully applied for the pharmacokinetic and bioavailability studies of the five pulchinenosides which are potentially active saponins present in P. chinensis saponins (PRS) extracts.

Authors+Show Affiliations

College of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China; Jiangxi University of Traditional Chinese Medicine, 56 Yangming Road, Nanchang 330006, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24269908

Citation

Liu, Yali, et al. "Validated Rapid Resolution LC-ESI-MS/MS Method for Simultaneous Determination of Five Pulchinenosides From Pulsatilla Chinensis (Bunge) Regel in Rat Plasma: Application to Pharmacokinetics and Bioavailability Studies." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 942-943, 2013, pp. 141-50.
Liu Y, Song Y, Xu Q, et al. Validated rapid resolution LC-ESI-MS/MS method for simultaneous determination of five pulchinenosides from Pulsatilla chinensis (Bunge) Regel in rat plasma: application to pharmacokinetics and bioavailability studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2013;942-943:141-50.
Liu, Y., Song, Y., Xu, Q., Su, D., Feng, Y., Li, X., Khan, I. A., Zhang, L., Chen, L., & Yang, S. (2013). Validated rapid resolution LC-ESI-MS/MS method for simultaneous determination of five pulchinenosides from Pulsatilla chinensis (Bunge) Regel in rat plasma: application to pharmacokinetics and bioavailability studies. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 942-943, 141-50. https://doi.org/10.1016/j.jchromb.2013.10.036
Liu Y, et al. Validated Rapid Resolution LC-ESI-MS/MS Method for Simultaneous Determination of Five Pulchinenosides From Pulsatilla Chinensis (Bunge) Regel in Rat Plasma: Application to Pharmacokinetics and Bioavailability Studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 30;942-943:141-50. PubMed PMID: 24269908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated rapid resolution LC-ESI-MS/MS method for simultaneous determination of five pulchinenosides from Pulsatilla chinensis (Bunge) Regel in rat plasma: application to pharmacokinetics and bioavailability studies. AU - Liu,Yali, AU - Song,Yonggui, AU - Xu,Qiongming, AU - Su,Dan, AU - Feng,Yulin, AU - Li,Xiang, AU - Khan,Ikhlas A, AU - Zhang,Ling, AU - Chen,Lanying, AU - Yang,Shilin, Y1 - 2013/11/02/ PY - 2013/01/01/received PY - 2013/10/21/revised PY - 2013/10/28/accepted PY - 2013/11/26/entrez PY - 2013/11/26/pubmed PY - 2014/7/7/medline KW - Bioavailability KW - Oleanane pulchinenosides KW - Pharmacokinetics KW - Pulsatilla chinensis KW - RRLC–ESI-MS/MS SP - 141 EP - 50 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 942-943 N2 - A simple, RRLC-ESI-MS/MS method was developed for the simultaneous determination of five oleanane pulchinenosides (B3, BD, B7, B10, and B11), in rat plasma following solid-phase extraction (SPE). Detection and quantitation were performed by MS/MS using electrospray ionization (ESI) and multiple reaction monitoring (MRM) mode. The MS/MS transitions of the triterpenoidal saponins: m/z 911.4→603.2, 749.4→471.3, 895.6→733.2, 733.5→455.3, and 579.3→371.1 were monitored for B3, BD, B7 and B10, B11 and internal standard (Forsythin), respectively. The method was validated in plasma samples, showed good linearity over a wide concentration range (r(2)>0.99), and with lower limits of quantification of 1.11 (B3), 0.751 (BD), 0.996 (B7), 0.415 (B10), and 0.332 (B11)ng/mL. The intra- and inter-day assay variability was less than 15% for all analytes. The mean extraction recoveries of analytes and IS from rats plasma were all more than 70.0%. The validation results demonstrate that this method is robust and specific. The validated method was successfully applied for the pharmacokinetic and bioavailability studies of the five pulchinenosides which are potentially active saponins present in P. chinensis saponins (PRS) extracts. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24269908/Validated_rapid_resolution_LC_ESI_MS/MS_method_for_simultaneous_determination_of_five_pulchinenosides_from_Pulsatilla_chinensis__Bunge__Regel_in_rat_plasma:_application_to_pharmacokinetics_and_bioavailability_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00596-5 DB - PRIME DP - Unbound Medicine ER -