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Profiling the main cell wall polysaccharides of grapevine leaves using high-throughput and fractionation methods.
Carbohydr Polym. 2014 Jan; 99:190-8.CP

Abstract

Vitis species include Vitis vinifera, the domesticated grapevine, used for wine and grape agricultural production and considered the world's most important fruit crop. A cell wall preparation, isolated from fully expanded photosynthetically active leaves, was fractionated via chemical and enzymatic reagents; and the various extracts obtained were assayed using high-throughput cell wall profiling tools according to a previously optimized and validated workflow. The bulk of the homogalacturonan-rich pectin present was efficiently extracted using CDTA treatment, whereas over half of the grapevine leaf cell wall consisted of vascular veins, comprised of xylans and cellulose. The main hemicellulose component was found to be xyloglucan and an enzymatic oligosaccharide fingerprinting approach was used to analyze the grapevine leaf xyloglucan fraction. When Paenibacillus sp. xyloglucanase was applied the main subunits released were XXFG and XLFG; whereas the less-specific Trichoderma reesei EGII was also able to release the XXXG motif as well as other oligomers likely of mannan and xylan origin. This latter enzyme would thus be useful to screen for xyloglucan, xylan and mannan-linked cell wall alterations in laboratory and field grapevine populations. This methodology is well-suited for high-throughput cell wall profiling of grapevine mutant and transgenic plants for investigating the range of biological processes, specifically plant disease studies and plant-pathogen interactions, where the cell wall plays a crucial role.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Moore JP
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland 7602, South Africa. Electronic address: moorejp@sun.ac.za.
Nguema-Ona E
No affiliation info available
Fangel JU
No affiliation info available
Willats WG
No affiliation info available
Hugo A
No affiliation info available
Vivier MA
No affiliation info available

MeSH

Bacterial ProteinsCell WallCelluloseChemical FractionationEdetic AcidFungal ProteinsGlucansGlycoside HydrolasesHigh-Throughput Screening AssaysMannansPaenibacillusPectinsPlant ExtractsPlant LeavesTrichodermaVitisXylans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24274496

Citation

Moore, John P., et al. "Profiling the Main Cell Wall Polysaccharides of Grapevine Leaves Using High-throughput and Fractionation Methods." Carbohydrate Polymers, vol. 99, 2014, pp. 190-8.
Moore JP, Nguema-Ona E, Fangel JU, et al. Profiling the main cell wall polysaccharides of grapevine leaves using high-throughput and fractionation methods. Carbohydr Polym. 2014;99:190-8.
Moore, J. P., Nguema-Ona, E., Fangel, J. U., Willats, W. G., Hugo, A., & Vivier, M. A. (2014). Profiling the main cell wall polysaccharides of grapevine leaves using high-throughput and fractionation methods. Carbohydrate Polymers, 99, 190-8. https://doi.org/10.1016/j.carbpol.2013.08.013
Moore JP, et al. Profiling the Main Cell Wall Polysaccharides of Grapevine Leaves Using High-throughput and Fractionation Methods. Carbohydr Polym. 2014;99:190-8. PubMed PMID: 24274496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profiling the main cell wall polysaccharides of grapevine leaves using high-throughput and fractionation methods. AU - Moore,John P, AU - Nguema-Ona,Eric, AU - Fangel,Jonatan U, AU - Willats,William G T, AU - Hugo,Annatjie, AU - Vivier,Melané A, Y1 - 2013/08/17/ PY - 2013/05/15/received PY - 2013/08/06/revised PY - 2013/08/07/accepted PY - 2013/11/27/entrez PY - 2013/11/28/pubmed PY - 2014/7/16/medline KW - AIR KW - CBM KW - Cell wall polysaccharides KW - CoMPP KW - EPG KW - ESI-MS KW - Endopolygalacturonase (EPG) KW - FT-IR KW - Fourier transform-infrared spectroscopy KW - Grapevine profiling KW - HG KW - HPAEC-PAD KW - Paenibacillus sp xyloglucan specific endoglucanase 5 KW - Pectin-rich KW - PspXEG5 KW - RG KW - TrEGII KW - Tricshoderma reesei endoglucanase II KW - XEG KW - XyG KW - Xyloglucan KW - Xyloglucan-specific endoglucanase (XEG) KW - alcohol insoluble residue KW - carbohydrate binding module KW - comprehensive microarray polymer profiling KW - electrospray ionization-mass spectrometry KW - endopolygalacturonase KW - high performance anion exchange chromatography-pulse amperometric detection KW - homogalacturonan KW - mAb KW - monoclonal antibody KW - rhamnogalacturonan KW - xyloglucan KW - xyloglucan-specific endoglucanase SP - 190 EP - 8 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 99 N2 - Vitis species include Vitis vinifera, the domesticated grapevine, used for wine and grape agricultural production and considered the world's most important fruit crop. A cell wall preparation, isolated from fully expanded photosynthetically active leaves, was fractionated via chemical and enzymatic reagents; and the various extracts obtained were assayed using high-throughput cell wall profiling tools according to a previously optimized and validated workflow. The bulk of the homogalacturonan-rich pectin present was efficiently extracted using CDTA treatment, whereas over half of the grapevine leaf cell wall consisted of vascular veins, comprised of xylans and cellulose. The main hemicellulose component was found to be xyloglucan and an enzymatic oligosaccharide fingerprinting approach was used to analyze the grapevine leaf xyloglucan fraction. When Paenibacillus sp. xyloglucanase was applied the main subunits released were XXFG and XLFG; whereas the less-specific Trichoderma reesei EGII was also able to release the XXXG motif as well as other oligomers likely of mannan and xylan origin. This latter enzyme would thus be useful to screen for xyloglucan, xylan and mannan-linked cell wall alterations in laboratory and field grapevine populations. This methodology is well-suited for high-throughput cell wall profiling of grapevine mutant and transgenic plants for investigating the range of biological processes, specifically plant disease studies and plant-pathogen interactions, where the cell wall plays a crucial role. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/24274496/Profiling_the_main_cell_wall_polysaccharides_of_grapevine_leaves_using_high_throughput_and_fractionation_methods_ DB - PRIME DP - Unbound Medicine ER -