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A novel AtKEA gene family, homolog of bacterial K+/H+ antiporters, plays potential roles in K+ homeostasis and osmotic adjustment in Arabidopsis.
PLoS One. 2013; 8(11):e81463.Plos

Abstract

AtKEAs, homologs of bacterial KefB/KefC, are predicted to encode K(+)/H(+) antiporters in Arabidopsis. The AtKEA family contains six genes forming two subgroups in the cladogram: AtKEA1-3 and AtKEA4-6. AtKEA1 and AtKEA2 have a long N-terminal domain; the full-length AtKEA1 was inactive in yeast. The transport activity was analyzed by expressing the AtKEA genes in yeast mutants lacking multiple ion carriers. AtKEAs conferred resistance to high K(+) and hygromycin B but not to salt and Li(+) stress. AtKEAs expressed in both the shoot and root of Arabidopsis. The expression of AtKEA1, -3 and -4 was enhanced under low K(+) stress, whereas AtKEA2 and AtKEA5 were induced by sorbitol and ABA treatments. However, osmotic induction of AtKEA2 and AtKEA5 was not observed in aba2-3 mutants, suggesting an ABA regulated mechanism for their osmotic response. AtKEAs' expression may not be regulated by the SOS pathway since their expression was not affected in sos mutants. The GFP tagging analysis showed that AtKEAs distributed diversely in yeast. The Golgi localization of AtKEA3 was demonstrated by both the stably transformed seedlings and the transient expression in protoplasts. Overall, AtKEAs expressed and localized diversely, and may play roles in K(+) homeostasis and osmotic adjustment in Arabidopsis.

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Authors+Show Affiliations

Zheng S
MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, China.
Pan T
No affiliation info available
Fan L
No affiliation info available
Qiu QS
No affiliation info available

MeSH

Amino Acid SequenceArabidopsisArabidopsis ProteinsBiological TransportGene ExpressionGene Expression Regulation, PlantHomeostasisHydrogen-Ion ConcentrationMolecular Sequence DataMultigene FamilyOsmosisPhylogenyPlant RootsPlant ShootsPlants, Genetically ModifiedPotassiumPotassium-Hydrogen AntiportersProtein TransportSequence AlignmentSignal TransductionSodium-Hydrogen ExchangersStress, PhysiologicalYeasts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24278440

Citation

Zheng, Sheng, et al. "A Novel AtKEA Gene Family, Homolog of Bacterial K+/H+ Antiporters, Plays Potential Roles in K+ Homeostasis and Osmotic Adjustment in Arabidopsis." PloS One, vol. 8, no. 11, 2013, pp. e81463.
Zheng S, Pan T, Fan L, et al. A novel AtKEA gene family, homolog of bacterial K+/H+ antiporters, plays potential roles in K+ homeostasis and osmotic adjustment in Arabidopsis. PLoS One. 2013;8(11):e81463.
Zheng, S., Pan, T., Fan, L., & Qiu, Q. S. (2013). A novel AtKEA gene family, homolog of bacterial K+/H+ antiporters, plays potential roles in K+ homeostasis and osmotic adjustment in Arabidopsis. PloS One, 8(11), e81463. https://doi.org/10.1371/journal.pone.0081463
Zheng S, et al. A Novel AtKEA Gene Family, Homolog of Bacterial K+/H+ Antiporters, Plays Potential Roles in K+ Homeostasis and Osmotic Adjustment in Arabidopsis. PLoS One. 2013;8(11):e81463. PubMed PMID: 24278440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel AtKEA gene family, homolog of bacterial K+/H+ antiporters, plays potential roles in K+ homeostasis and osmotic adjustment in Arabidopsis. AU - Zheng,Sheng, AU - Pan,Ting, AU - Fan,Ligang, AU - Qiu,Quan-Sheng, Y1 - 2013/11/20/ PY - 2013/09/08/received PY - 2013/10/13/accepted PY - 2013/11/27/entrez PY - 2013/11/28/pubmed PY - 2015/1/3/medline SP - e81463 EP - e81463 JF - PloS one JO - PLoS One VL - 8 IS - 11 N2 - AtKEAs, homologs of bacterial KefB/KefC, are predicted to encode K(+)/H(+) antiporters in Arabidopsis. The AtKEA family contains six genes forming two subgroups in the cladogram: AtKEA1-3 and AtKEA4-6. AtKEA1 and AtKEA2 have a long N-terminal domain; the full-length AtKEA1 was inactive in yeast. The transport activity was analyzed by expressing the AtKEA genes in yeast mutants lacking multiple ion carriers. AtKEAs conferred resistance to high K(+) and hygromycin B but not to salt and Li(+) stress. AtKEAs expressed in both the shoot and root of Arabidopsis. The expression of AtKEA1, -3 and -4 was enhanced under low K(+) stress, whereas AtKEA2 and AtKEA5 were induced by sorbitol and ABA treatments. However, osmotic induction of AtKEA2 and AtKEA5 was not observed in aba2-3 mutants, suggesting an ABA regulated mechanism for their osmotic response. AtKEAs' expression may not be regulated by the SOS pathway since their expression was not affected in sos mutants. The GFP tagging analysis showed that AtKEAs distributed diversely in yeast. The Golgi localization of AtKEA3 was demonstrated by both the stably transformed seedlings and the transient expression in protoplasts. Overall, AtKEAs expressed and localized diversely, and may play roles in K(+) homeostasis and osmotic adjustment in Arabidopsis. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24278440/A_novel_AtKEA_gene_family_homolog_of_bacterial_K+/H+_antiporters_plays_potential_roles_in_K+_homeostasis_and_osmotic_adjustment_in_Arabidopsis_ DB - PRIME DP - Unbound Medicine ER -