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Ultra(high)-pressure liquid chromatography-electrospray ionization-time-of-flight-ion mobility-high definition mass spectrometry for the rapid identification and structural characterization of flavonoid glycosides from cauliflower waste.
J Chromatogr A. 2014 Jan 03; 1323:39-48.JC

Abstract

In this paper, a strategy for the detection and structural elucidation of flavonoid glycosides from a complex matrix in a single chromatographic run using U(H)PLC-ESI-IMS-HDMS/MS(E) is presented. This system operates using alternative low and high energy voltages that is able to perform the task of conventional MS/MS in a data-independent way without re-injection of the sample, which saves analytical time. Also, ion mobility separation (IMS) was employed as an additional separation technique for compounds that are co-eluting after U(H)PLC separation. First, the fragmentation of flavonoid standards were analyzed and criteria was set for structural elucidation of flavonoids in a plant extract. Based on retention times, UV spectra, exact mass, and MS fragment characteristics, such as abundances of daughter ions and the presence of radical ions ([Y0-H](-)), a total 19 flavonoid glycosides, of which 8 non-acylated and 11 acylated, were detected and structurally characterized in a cauliflower waste extract. Kaempferol and quercetin were the main aglycones detected while sinapic and ferulic acid were the main phenolic acids. C-glycosides were also found although their structure could not be elucidated. The proposed method can be used as a rapid screening test for flavonoid identification and for routine analysis of plant extracts, such as these derived from cauliflower waste. The study also confirms that agroindustrial wastes, such as cauliflower leaves, could be seen as a valuable source of different bioactive phenolic compounds.

Authors+Show Affiliations

Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Belgium; Department of Industrial Engineering and Technology, University College West Flanders, Kortrijk, Belgium; Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Belgium.Department of Industrial Engineering and Technology, University College West Flanders, Kortrijk, Belgium; Department of Industrial Biological Science, Faculty of Bioscience Engineering, Ghent University, Belgium.Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Belgium.Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Belgium.Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Belgium.Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Belgium. Electronic address: John.VanCamp@UGent.be.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24280615

Citation

Gonzales, Gerard Bryan, et al. "Ultra(high)-pressure Liquid Chromatography-electrospray Ionization-time-of-flight-ion Mobility-high Definition Mass Spectrometry for the Rapid Identification and Structural Characterization of Flavonoid Glycosides From Cauliflower Waste." Journal of Chromatography. A, vol. 1323, 2014, pp. 39-48.
Gonzales GB, Raes K, Coelus S, et al. Ultra(high)-pressure liquid chromatography-electrospray ionization-time-of-flight-ion mobility-high definition mass spectrometry for the rapid identification and structural characterization of flavonoid glycosides from cauliflower waste. J Chromatogr A. 2014;1323:39-48.
Gonzales, G. B., Raes, K., Coelus, S., Struijs, K., Smagghe, G., & Van Camp, J. (2014). Ultra(high)-pressure liquid chromatography-electrospray ionization-time-of-flight-ion mobility-high definition mass spectrometry for the rapid identification and structural characterization of flavonoid glycosides from cauliflower waste. Journal of Chromatography. A, 1323, 39-48. https://doi.org/10.1016/j.chroma.2013.10.077
Gonzales GB, et al. Ultra(high)-pressure Liquid Chromatography-electrospray Ionization-time-of-flight-ion Mobility-high Definition Mass Spectrometry for the Rapid Identification and Structural Characterization of Flavonoid Glycosides From Cauliflower Waste. J Chromatogr A. 2014 Jan 3;1323:39-48. PubMed PMID: 24280615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra(high)-pressure liquid chromatography-electrospray ionization-time-of-flight-ion mobility-high definition mass spectrometry for the rapid identification and structural characterization of flavonoid glycosides from cauliflower waste. AU - Gonzales,Gerard Bryan, AU - Raes,Katleen, AU - Coelus,Sofie, AU - Struijs,Karin, AU - Smagghe,Guy, AU - Van Camp,John, Y1 - 2013/11/07/ PY - 2013/08/26/received PY - 2013/10/18/revised PY - 2013/10/24/accepted PY - 2013/11/28/entrez PY - 2013/11/28/pubmed PY - 2014/9/23/medline KW - Brassica waste stream KW - Flavonoids KW - Ion mobility KW - Mass spectrometry SP - 39 EP - 48 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1323 N2 - In this paper, a strategy for the detection and structural elucidation of flavonoid glycosides from a complex matrix in a single chromatographic run using U(H)PLC-ESI-IMS-HDMS/MS(E) is presented. This system operates using alternative low and high energy voltages that is able to perform the task of conventional MS/MS in a data-independent way without re-injection of the sample, which saves analytical time. Also, ion mobility separation (IMS) was employed as an additional separation technique for compounds that are co-eluting after U(H)PLC separation. First, the fragmentation of flavonoid standards were analyzed and criteria was set for structural elucidation of flavonoids in a plant extract. Based on retention times, UV spectra, exact mass, and MS fragment characteristics, such as abundances of daughter ions and the presence of radical ions ([Y0-H](-)), a total 19 flavonoid glycosides, of which 8 non-acylated and 11 acylated, were detected and structurally characterized in a cauliflower waste extract. Kaempferol and quercetin were the main aglycones detected while sinapic and ferulic acid were the main phenolic acids. C-glycosides were also found although their structure could not be elucidated. The proposed method can be used as a rapid screening test for flavonoid identification and for routine analysis of plant extracts, such as these derived from cauliflower waste. The study also confirms that agroindustrial wastes, such as cauliflower leaves, could be seen as a valuable source of different bioactive phenolic compounds. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/24280615/Ultra_high__pressure_liquid_chromatography_electrospray_ionization_time_of_flight_ion_mobility_high_definition_mass_spectrometry_for_the_rapid_identification_and_structural_characterization_of_flavonoid_glycosides_from_cauliflower_waste_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(13)01715-9 DB - PRIME DP - Unbound Medicine ER -