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Interconnectivity of sympathetic and sleep networks is mediated through reduction of gamma aminobutyric acidergic inhibition in the paraventricular nucleus.
J Sleep Res. 2014 Apr; 23(2):168-75.JS

Abstract

Chronic short sleep duration has been linked to sympathoexcitation and increased risk of cardiovascular disease. The central nervous system plays an important role in the regulation of sympathetic activity. Thus, the present study evaluates the pre-autonomic neurones in the paraventricular nucleus of the hypothalamus and rostral ventrolateral medulla after sleep restriction using various protein expression measurements and electrophysiological approaches. Wistar male rats were assigned randomly to two experimental groups: control or sleep restriction for 14 days. Sleep restriction was defined as 20 h of paradoxical sleep deprivation followed by a 4 h sleep permission period using the modified multiple platform method. Micropunches of the paraventricular nucleus of the hypothalamus and rostral ventrolateral medulla were dissected to evaluate the protein expression of angiotensin II receptor, type 1 (AT1), AT2, gamma aminobutyric acidA) (N-methyl-d-aspartate receptor1) and neuronal nitric oxide synthase neuronal nitric oxide synthase isoform through immunoblotting. Sleep restriction induced a down-regulation of the gamma aminobutyric acidA receptor in the paraventricular nucleus of the hypothalamus. Microinjection of bicuculline, a gamma aminobutyric acid receptor blocker, into the paraventricular nucleus of the hypothalamus increased renal sympathetic activity renal sympathetic nerve activity, mean arterial pressure and heart rate in anaesthetized control rats. However, the amplitude and frequency of renal sympathetic nerve activity was higher in the sleep restriction group. These findings suggest that gamma aminobutyric acidergic inhibition within the paraventricular nucleus of the hypothalamus is involved in sympathoexcitation induced by sleep restriction.

Authors+Show Affiliations

Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24283672

Citation

Perry, Juliana C., et al. "Interconnectivity of Sympathetic and Sleep Networks Is Mediated Through Reduction of Gamma Aminobutyric Acidergic Inhibition in the Paraventricular Nucleus." Journal of Sleep Research, vol. 23, no. 2, 2014, pp. 168-75.
Perry JC, Bergamaschi CT, Campos RR, et al. Interconnectivity of sympathetic and sleep networks is mediated through reduction of gamma aminobutyric acidergic inhibition in the paraventricular nucleus. J Sleep Res. 2014;23(2):168-75.
Perry, J. C., Bergamaschi, C. T., Campos, R. R., Silva, A. M., & Tufik, S. (2014). Interconnectivity of sympathetic and sleep networks is mediated through reduction of gamma aminobutyric acidergic inhibition in the paraventricular nucleus. Journal of Sleep Research, 23(2), 168-75. https://doi.org/10.1111/jsr.12110
Perry JC, et al. Interconnectivity of Sympathetic and Sleep Networks Is Mediated Through Reduction of Gamma Aminobutyric Acidergic Inhibition in the Paraventricular Nucleus. J Sleep Res. 2014;23(2):168-75. PubMed PMID: 24283672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interconnectivity of sympathetic and sleep networks is mediated through reduction of gamma aminobutyric acidergic inhibition in the paraventricular nucleus. AU - Perry,Juliana C, AU - Bergamaschi,Cássia T, AU - Campos,Ruy R, AU - Silva,Adilson M, AU - Tufik,Sergio, Y1 - 2013/11/28/ PY - 2013/02/06/received PY - 2013/10/12/accepted PY - 2013/11/29/entrez PY - 2013/11/29/pubmed PY - 2014/6/10/medline KW - cardiovascular system KW - gamma aminobutyric acid KW - paraventricular nucleus KW - sleep KW - sleep restriction KW - sympathetic activity SP - 168 EP - 75 JF - Journal of sleep research JO - J Sleep Res VL - 23 IS - 2 N2 - Chronic short sleep duration has been linked to sympathoexcitation and increased risk of cardiovascular disease. The central nervous system plays an important role in the regulation of sympathetic activity. Thus, the present study evaluates the pre-autonomic neurones in the paraventricular nucleus of the hypothalamus and rostral ventrolateral medulla after sleep restriction using various protein expression measurements and electrophysiological approaches. Wistar male rats were assigned randomly to two experimental groups: control or sleep restriction for 14 days. Sleep restriction was defined as 20 h of paradoxical sleep deprivation followed by a 4 h sleep permission period using the modified multiple platform method. Micropunches of the paraventricular nucleus of the hypothalamus and rostral ventrolateral medulla were dissected to evaluate the protein expression of angiotensin II receptor, type 1 (AT1), AT2, gamma aminobutyric acidA) (N-methyl-d-aspartate receptor1) and neuronal nitric oxide synthase neuronal nitric oxide synthase isoform through immunoblotting. Sleep restriction induced a down-regulation of the gamma aminobutyric acidA receptor in the paraventricular nucleus of the hypothalamus. Microinjection of bicuculline, a gamma aminobutyric acid receptor blocker, into the paraventricular nucleus of the hypothalamus increased renal sympathetic activity renal sympathetic nerve activity, mean arterial pressure and heart rate in anaesthetized control rats. However, the amplitude and frequency of renal sympathetic nerve activity was higher in the sleep restriction group. These findings suggest that gamma aminobutyric acidergic inhibition within the paraventricular nucleus of the hypothalamus is involved in sympathoexcitation induced by sleep restriction. SN - 1365-2869 UR - https://www.unboundmedicine.com/medline/citation/24283672/Interconnectivity_of_sympathetic_and_sleep_networks_is_mediated_through_reduction_of_gamma_aminobutyric_acidergic_inhibition_in_the_paraventricular_nucleus_ L2 - https://doi.org/10.1111/jsr.12110 DB - PRIME DP - Unbound Medicine ER -