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A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice.
J Ethnopharmacol 2014; 151(1):675-85JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Refined-JQ (JQ-R) is a mixture of refined extracts from three major herbal components of JinQi-JiangTang tablet: Coptis chinensis (Ranunculaceae), Astragalus membranaceus (Leguminosae), and Lonicera japonica (Caprifoliaceae). Our previous studies have indicated that JQ-R could decrease fasting blood glucose levels in diabetic mice and insulin resistance mice. Investigating the hypoglycemic effect of JQ-R on prediabetes has practical application value for preventing or delaying insulin resistance, impaired glucose tolerance and possibly the development of clinical diabetes.

MATERIALS AND METHODS

The anti-diabetic potential of JQ-R was investigated using a high fat-diet (HFD)-induced obesity mouse model. C57BL/6J mice (HFD-C57 mice) were fed with high-fat diet for 4 months. HFD-C57 mice were treated with either JQ-R (administered intragastrically once daily for 4 weeks) or metformin (as positive control), and the effects of JQ-R on body weight, blood lipids, glucose metabolism, insulin sensitivity, and beta cell function were monitored.

RESULTS

The body weight, serum cholesterol, and the Homeostasis Model Assessment ratio (insulin resistance index) were significantly reduced in JQ-R or metformin-treated mice, and the glucose tolerance was enhanced and insulin response was improved simultaneously. Moreover, both JQ-R and metformin could activate liver glycogen syntheses even under a relatively high glucose loading. Although glyconeogenesis was inhibited in the metformin treated mice, it was not observed in JQ-R treated mice. Similar to metformin, JQ-R could also improve the glucose infusion rate (GIR) in hyperglycemic clamp test. JQ-R was also shown to increase the levels of phosphorylated AMPKα and phosphorylated acetyl CoA carboxylase (ACC), similar to metformin.

CONCLUSION

JQ-R could reduce HFD-induced insulin resistance by regulating glucose and lipid metabolism, increasing insulin sensitivity through activating the AMPK signaling pathway, and subsequently improving β cell function. Therefore, JQ-R may offer an alternative in treating disorders associated with insulin resistance, such as prediabetes and T2DM.

Authors+Show Affiliations

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China.State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xiannongtan Street, Beijing 100050, PR China. Electronic address: shenzhf@imm.ac.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24286962

Citation

Gao, Li-hui, et al. "A refined-JinQi-JiangTang Tablet Ameliorates Prediabetes By Reducing Insulin Resistance and Improving Beta Cell Function in Mice." Journal of Ethnopharmacology, vol. 151, no. 1, 2014, pp. 675-85.
Gao LH, Liu Q, Liu SN, et al. A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice. J Ethnopharmacol. 2014;151(1):675-85.
Gao, L. H., Liu, Q., Liu, S. N., Chen, Z. Y., Li, C. N., Lei, L., ... Shen, Z. F. (2014). A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice. Journal of Ethnopharmacology, 151(1), pp. 675-85. doi:10.1016/j.jep.2013.11.024.
Gao LH, et al. A refined-JinQi-JiangTang Tablet Ameliorates Prediabetes By Reducing Insulin Resistance and Improving Beta Cell Function in Mice. J Ethnopharmacol. 2014;151(1):675-85. PubMed PMID: 24286962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A refined-JinQi-JiangTang tablet ameliorates prediabetes by reducing insulin resistance and improving beta cell function in mice. AU - Gao,Li-hui, AU - Liu,Quan, AU - Liu,Shuai-nan, AU - Chen,Zhi-yu, AU - Li,Cai-na, AU - Lei,Lei, AU - Sun,Su-juan, AU - Li,Lin-yi, AU - Liu,Jing-long, AU - Shen,Zhu-fang, Y1 - 2013/11/25/ PY - 2013/04/19/received PY - 2013/11/11/revised PY - 2013/11/13/accepted PY - 2013/11/30/entrez PY - 2013/11/30/pubmed PY - 2014/9/10/medline KW - ACC KW - AMPK KW - AUC KW - Beta cell function KW - CON KW - ELSD KW - Evaporative Light Scattering Detector KW - GIR KW - GLUT2 KW - Glucose transporter 2 KW - HFD KW - HOMA-IR KW - Herbal effective fractions KW - High fat diet KW - Homeostasis Model Assessment ratio of insulin resistance KW - IFG KW - IGT KW - IOD KW - IR KW - ITT KW - Insulin resistance KW - JQ-R KW - MET KW - NOR KW - OGTT KW - Prediabetes KW - Refined-JinQi-JiangTang tablet KW - S.E.M KW - T2DM KW - TC KW - TG KW - Type 2 Diabetes Mellitus KW - acetyl CoA carboxylase KW - adenosine monophosphate-activated protein kinase KW - area under the curves KW - glucose infusion rate KW - group treated with metformin KW - high fat-diet KW - impaired fasting glucose KW - impaired glucose tolerance KW - insulin resistance KW - insulin tolerance test KW - integrated optical density KW - normal control KW - oral glucose tolerance test KW - standard error of mean KW - total cholesterol KW - total triglyceride KW - untreated control group SP - 675 EP - 85 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 151 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Refined-JQ (JQ-R) is a mixture of refined extracts from three major herbal components of JinQi-JiangTang tablet: Coptis chinensis (Ranunculaceae), Astragalus membranaceus (Leguminosae), and Lonicera japonica (Caprifoliaceae). Our previous studies have indicated that JQ-R could decrease fasting blood glucose levels in diabetic mice and insulin resistance mice. Investigating the hypoglycemic effect of JQ-R on prediabetes has practical application value for preventing or delaying insulin resistance, impaired glucose tolerance and possibly the development of clinical diabetes. MATERIALS AND METHODS: The anti-diabetic potential of JQ-R was investigated using a high fat-diet (HFD)-induced obesity mouse model. C57BL/6J mice (HFD-C57 mice) were fed with high-fat diet for 4 months. HFD-C57 mice were treated with either JQ-R (administered intragastrically once daily for 4 weeks) or metformin (as positive control), and the effects of JQ-R on body weight, blood lipids, glucose metabolism, insulin sensitivity, and beta cell function were monitored. RESULTS: The body weight, serum cholesterol, and the Homeostasis Model Assessment ratio (insulin resistance index) were significantly reduced in JQ-R or metformin-treated mice, and the glucose tolerance was enhanced and insulin response was improved simultaneously. Moreover, both JQ-R and metformin could activate liver glycogen syntheses even under a relatively high glucose loading. Although glyconeogenesis was inhibited in the metformin treated mice, it was not observed in JQ-R treated mice. Similar to metformin, JQ-R could also improve the glucose infusion rate (GIR) in hyperglycemic clamp test. JQ-R was also shown to increase the levels of phosphorylated AMPKα and phosphorylated acetyl CoA carboxylase (ACC), similar to metformin. CONCLUSION: JQ-R could reduce HFD-induced insulin resistance by regulating glucose and lipid metabolism, increasing insulin sensitivity through activating the AMPK signaling pathway, and subsequently improving β cell function. Therefore, JQ-R may offer an alternative in treating disorders associated with insulin resistance, such as prediabetes and T2DM. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/24286962/A_refined_JinQi_JiangTang_tablet_ameliorates_prediabetes_by_reducing_insulin_resistance_and_improving_beta_cell_function_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00818-0 DB - PRIME DP - Unbound Medicine ER -