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Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia.
BMC Complement Altern Med. 2013 Dec 01; 13:341.BC

Abstract

BACKGROUND

Phytochemicals and antioxidants from plant sources are of increasing interest to consumers because of their roles in the maintenance of human health. Most of the secondary metabolites of herbs are used in a number of pharmaceutical products.

METHODS

Secondary metabolites composition and content of five flavonoids and three phenolic acids were evaluated and determined in Pandanus amaryllifolius extracts from three different locations of Malaysia by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau and aluminum chloride colorimetric assay; The antioxidant activity of the extracts was determined by the ferric reducing antioxidant potential (FRAP) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) Assay was employed to screen anticancer activity of extracts against MCF-7 cancer cell line.

RESULTS

Highest value of total flavonoids (TF) and total phenolics (TP) was observed in pandan extract from Bachok locattion (1.87 mg/g DW and 6.72 mg/g DW) followed by Klang (1.32 mg/g DW; 5.07 mg/g DW) and Pontian (1.12 mg/g DW; 4.88 mg/g DW). Rutin just detected from Bachok location with value of 0.082 mg/g DW. High content of epicatechin (0.035 mg/g DW) and naringin (0.325 mg/g DW) were observed from Bachok location while, highest content of catechin (0.613 mg/g DW) and kaempferol (0.278 mg/g DW) was observed in pandan extract from Klang location. The extract of pandan from Bachok exhibited highest value of gallic acid (0.423 mg/g DW) and cinnamic acid (0.084 mg/g DW). Ferrulic acid just detected from pandan extract of Bachok location with concentration of 0.281 mg/g DW. Between studied locations Bachok exhibited highest value of DPPH (64.27%) and FRAP (517.2 μm of Fe (II)/g) activity followed by Klang (52.16%; 448.6 μm of Fe (II)/g) and Pontian (50.10%; 314.8 μm of Fe (II)/g). The preliminary screening showed pandan extracts from 3 locations possessed anticancer promoting activity against MCF-7 cell line, with 78.3%, 70.5% and 67.4% inhibition rate, respectively. Maximum MCF-7cell line inhibition was observed in pandan extract from Bachok location.

CONCLUSIONS

The samples collected from the North (Bachok) exhibited the highest TP, TF antioxidant and anticancer activity while those from the Southern portion (Pontian) appeared to have the lowest content of TP, TF and antioxidant activity.

Authors+Show Affiliations

Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia. alighasemzadeh@upm.edu.my.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24289290

Citation

Ghasemzadeh, Ali, and Hawa Z E. Jaafar. "Profiling of Phenolic Compounds and Their Antioxidant and Anticancer Activities in Pandan (Pandanus Amaryllifolius Roxb.) Extracts From Different Locations of Malaysia." BMC Complementary and Alternative Medicine, vol. 13, 2013, p. 341.
Ghasemzadeh A, Jaafar HZ. Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia. BMC Complement Altern Med. 2013;13:341.
Ghasemzadeh, A., & Jaafar, H. Z. (2013). Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia. BMC Complementary and Alternative Medicine, 13, 341. https://doi.org/10.1186/1472-6882-13-341
Ghasemzadeh A, Jaafar HZ. Profiling of Phenolic Compounds and Their Antioxidant and Anticancer Activities in Pandan (Pandanus Amaryllifolius Roxb.) Extracts From Different Locations of Malaysia. BMC Complement Altern Med. 2013 Dec 1;13:341. PubMed PMID: 24289290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia. AU - Ghasemzadeh,Ali, AU - Jaafar,Hawa Z E, Y1 - 2013/12/01/ PY - 2013/06/03/received PY - 2013/11/21/accepted PY - 2013/12/3/entrez PY - 2013/12/3/pubmed PY - 2014/5/14/medline SP - 341 EP - 341 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 13 N2 - BACKGROUND: Phytochemicals and antioxidants from plant sources are of increasing interest to consumers because of their roles in the maintenance of human health. Most of the secondary metabolites of herbs are used in a number of pharmaceutical products. METHODS: Secondary metabolites composition and content of five flavonoids and three phenolic acids were evaluated and determined in Pandanus amaryllifolius extracts from three different locations of Malaysia by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau and aluminum chloride colorimetric assay; The antioxidant activity of the extracts was determined by the ferric reducing antioxidant potential (FRAP) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) Assay was employed to screen anticancer activity of extracts against MCF-7 cancer cell line. RESULTS: Highest value of total flavonoids (TF) and total phenolics (TP) was observed in pandan extract from Bachok locattion (1.87 mg/g DW and 6.72 mg/g DW) followed by Klang (1.32 mg/g DW; 5.07 mg/g DW) and Pontian (1.12 mg/g DW; 4.88 mg/g DW). Rutin just detected from Bachok location with value of 0.082 mg/g DW. High content of epicatechin (0.035 mg/g DW) and naringin (0.325 mg/g DW) were observed from Bachok location while, highest content of catechin (0.613 mg/g DW) and kaempferol (0.278 mg/g DW) was observed in pandan extract from Klang location. The extract of pandan from Bachok exhibited highest value of gallic acid (0.423 mg/g DW) and cinnamic acid (0.084 mg/g DW). Ferrulic acid just detected from pandan extract of Bachok location with concentration of 0.281 mg/g DW. Between studied locations Bachok exhibited highest value of DPPH (64.27%) and FRAP (517.2 μm of Fe (II)/g) activity followed by Klang (52.16%; 448.6 μm of Fe (II)/g) and Pontian (50.10%; 314.8 μm of Fe (II)/g). The preliminary screening showed pandan extracts from 3 locations possessed anticancer promoting activity against MCF-7 cell line, with 78.3%, 70.5% and 67.4% inhibition rate, respectively. Maximum MCF-7cell line inhibition was observed in pandan extract from Bachok location. CONCLUSIONS: The samples collected from the North (Bachok) exhibited the highest TP, TF antioxidant and anticancer activity while those from the Southern portion (Pontian) appeared to have the lowest content of TP, TF and antioxidant activity. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/24289290/Profiling_of_phenolic_compounds_and_their_antioxidant_and_anticancer_activities_in_pandan__Pandanus_amaryllifolius_Roxb___extracts_from_different_locations_of_Malaysia_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-13-341 DB - PRIME DP - Unbound Medicine ER -