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Study of genetic damage in the Japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of DNA damage.
Aquat Toxicol. 2014 Jan; 146:93-104.AT

Abstract

Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg L(-1)) during the gametogenesis period. Genomic abnormalities were characterized using two complementary approaches. The Comet assay was applied for the measurement of early and reversible primary DNA damage, whereas flow cytometry was used to assess the clastogenic and aneugenic effect of diuron exposure. Polar Organic Chemical Integrative Samplers (POCIS) were used in exposed and assay tanks to confirm the waterborne concentration of diuron reached during the experiment. The results obtained by the Comet assay clearly showed a higher level of DNA strand breaks in both the hemocytes and spermatozoa of diuron-exposed genitors. The transmission of damaged genetic material to gamete cells could be responsible for the genetic damage measured in offspring. Indeed, flow cytometry analyses showed the presence of DNA breakage and a significant decrease in DNA content in spat from diuron-exposed genitors. The transmission of DNA damage to the offspring could be involved in the negative effects observed on offspring development (decrease in hatching rate, higher level of larval abnormalities, delay in metamorphosis) and growth. In this study, the vertical transmission of DNA damage was so highlighted by subjecting oyster genitors to short exposures to diuron at medium environmental concentrations. The analysis of POCIS showed that oysters were exposed to integrated concentrations as low as 0.2 and 0.3 μg L(-1), emphasizing the relevance of the results obtained and the risk associated to chemical contamination for oyster recruitment and fitness.

Authors+Show Affiliations

Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, F-17390 La Tremblade, France; Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, F-44311 Nantes Cedex 03, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, F-44311 Nantes Cedex 03, France. Electronic address: fakcha@ifremer.fr.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, F-44311 Nantes Cedex 03, France.Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, F-17390 La Tremblade, France.Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, F-17390 La Tremblade, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, F-44311 Nantes Cedex 03, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, F-44311 Nantes Cedex 03, France.University of Bordeaux, EPOC UMR CNRS 5805, F-33400 Talence, France.University of Bordeaux, EPOC UMR CNRS 5805, F-33400 Talence, France.Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, F-44311 Nantes Cedex 03, France.Ifremer, SG2M, Laboratory of Genetics and Pathology of Marine Molluscs, Avenue de Mus du Loup, F-17390 La Tremblade, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24291084

Citation

Barranger, A, et al. "Study of Genetic Damage in the Japanese Oyster Induced By an Environmentally-relevant Exposure to Diuron: Evidence of Vertical Transmission of DNA Damage." Aquatic Toxicology (Amsterdam, Netherlands), vol. 146, 2014, pp. 93-104.
Barranger A, Akcha F, Rouxel J, et al. Study of genetic damage in the Japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of DNA damage. Aquat Toxicol. 2014;146:93-104.
Barranger, A., Akcha, F., Rouxel, J., Brizard, R., Maurouard, E., Pallud, M., Menard, D., Tapie, N., Budzinski, H., Burgeot, T., & Benabdelmouna, A. (2014). Study of genetic damage in the Japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of DNA damage. Aquatic Toxicology (Amsterdam, Netherlands), 146, 93-104. https://doi.org/10.1016/j.aquatox.2013.10.032
Barranger A, et al. Study of Genetic Damage in the Japanese Oyster Induced By an Environmentally-relevant Exposure to Diuron: Evidence of Vertical Transmission of DNA Damage. Aquat Toxicol. 2014;146:93-104. PubMed PMID: 24291084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of genetic damage in the Japanese oyster induced by an environmentally-relevant exposure to diuron: evidence of vertical transmission of DNA damage. AU - Barranger,A, AU - Akcha,F, AU - Rouxel,J, AU - Brizard,R, AU - Maurouard,E, AU - Pallud,M, AU - Menard,D, AU - Tapie,N, AU - Budzinski,H, AU - Burgeot,T, AU - Benabdelmouna,A, Y1 - 2013/11/07/ PY - 2013/08/04/received PY - 2013/10/17/revised PY - 2013/10/29/accepted PY - 2013/12/3/entrez PY - 2013/12/3/pubmed PY - 2014/4/17/medline KW - Comet assay KW - Cytogenetics KW - Diuron KW - Genotoxicity KW - Herbicide KW - Oyster SP - 93 EP - 104 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 146 N2 - Pesticides represent a major proportion of the chemical pollutants detected in French coastal waters and hence a significant environmental risk with regards to marine organisms. Commercially-raised bivalves are particularly exposed to pollutants, among them pesticides, as shellfish farming zones are subject to considerable pressure from agricultural activities on the mainland. The aims of this study were to determine (1) the genotoxic effects of diuron exposure on oyster genitors and (2) the possible transmission of damaged DNA to offspring and its repercussions on oyster fitness. To investigate these points, oysters were exposed to concentrations of diuron close to those detected in the Marennes-Oleron Basin (two 7-day exposure pulses at 0.4 and 0.6 μg L(-1)) during the gametogenesis period. Genomic abnormalities were characterized using two complementary approaches. The Comet assay was applied for the measurement of early and reversible primary DNA damage, whereas flow cytometry was used to assess the clastogenic and aneugenic effect of diuron exposure. Polar Organic Chemical Integrative Samplers (POCIS) were used in exposed and assay tanks to confirm the waterborne concentration of diuron reached during the experiment. The results obtained by the Comet assay clearly showed a higher level of DNA strand breaks in both the hemocytes and spermatozoa of diuron-exposed genitors. The transmission of damaged genetic material to gamete cells could be responsible for the genetic damage measured in offspring. Indeed, flow cytometry analyses showed the presence of DNA breakage and a significant decrease in DNA content in spat from diuron-exposed genitors. The transmission of DNA damage to the offspring could be involved in the negative effects observed on offspring development (decrease in hatching rate, higher level of larval abnormalities, delay in metamorphosis) and growth. In this study, the vertical transmission of DNA damage was so highlighted by subjecting oyster genitors to short exposures to diuron at medium environmental concentrations. The analysis of POCIS showed that oysters were exposed to integrated concentrations as low as 0.2 and 0.3 μg L(-1), emphasizing the relevance of the results obtained and the risk associated to chemical contamination for oyster recruitment and fitness. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/24291084/Study_of_genetic_damage_in_the_Japanese_oyster_induced_by_an_environmentally_relevant_exposure_to_diuron:_evidence_of_vertical_transmission_of_DNA_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(13)00302-0 DB - PRIME DP - Unbound Medicine ER -