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Polymorphisms of endothelin 1 (G5665T and T-1370G) and endothelin receptor type A (C+70G and G-231A) in Graves' disease.
Int Immunopharmacol. 2014 Jan; 18(1):198-202.II

Abstract

PURPOSE

Endothelin 1 (EDN1) is a strong angiogenic and mitogenic factor, playing a key role in hypervascularization, thyroid follicle cell hyperplasia, and lymphocyte infiltration in the thyroid gland of patients with Graves' disease (GD). EDN1 induces angiogenesis and mitogenesis via endothelin receptor type A (EDNRA). This study examined the possible association of EDN1 (G5665T and T-1370G) and EDNRA (C+70G and G-231A) single nucleotide polymorphisms (SNPs) with the occurrence of GD, and evaluates the relationship between genotypes and clinical/laboratory manifestations of GD.

MATERIALS AND METHODS

We analyzed genotype and allele distributions of EDN1 and EDNRA polymorphisms in 165 patients with GD and 181 healthy controls by real-time PCR combined with melting curve analysis.

RESULTS

No significant associations between GD and variant alleles of the studied polymorphisms were observed. However, the anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG) levels in EDN1 G5665T GG genotype were higher than those in T allele carriers (GT+TT) (p=0.001 and p=0.026, respectively). In addition, anti-TPO levels in EDN1 T-1370G wild-type homozygous patients were found to be higher than in mutant gene carrying patients (GT+GG) (p=0.006). The presence of EDNRA+70G allele was associated with 3.37-fold increased risk for development of ophthalmopathy in GD patients (p=0.009).

CONCLUSION

Although there were no associations between EDN1 (G5665T and T-1370G) and EDNRA (C+70G and G-231A) SNPs and susceptibility to GD, EDN1 G5665T and T-1370G polymorphisms were related to alterations of autoantibody production and EDNRA C+70G polymorphism is related with increased risk for ophthalmopathy in GD patients.

Authors+Show Affiliations

Istanbul University, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul, Turkey.Istanbul University, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul, Turkey.Istanbul University, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul, Turkey.Istanbul University, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul, Turkey. Electronic address: pervinvural@yahoo.com.Şişli Etfal Education and Research Hospital, II. Internal Medicine Clinic, Department of Endocrinology, Şişli, 34387 Istanbul, Turkey.Şişli Etfal Education and Research Hospital, II. Internal Medicine Clinic, Department of Endocrinology, Şişli, 34387 Istanbul, Turkey.Istanbul University, Istanbul Faculty of Medicine, Department of Biochemistry, Istanbul, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24291390

Citation

Aydın, A Fatih, et al. "Polymorphisms of Endothelin 1 (G5665T and T-1370G) and Endothelin Receptor Type a (C+70G and G-231A) in Graves' Disease." International Immunopharmacology, vol. 18, no. 1, 2014, pp. 198-202.
Aydın AF, Develi-İş S, Doğru-Abbasoğlu S, et al. Polymorphisms of endothelin 1 (G5665T and T-1370G) and endothelin receptor type A (C+70G and G-231A) in Graves' disease. Int Immunopharmacol. 2014;18(1):198-202.
Aydın, A. F., Develi-İş, S., Doğru-Abbasoğlu, S., Vural, P., Ozderya, A., Karadağ, B., & Uysal, M. (2014). Polymorphisms of endothelin 1 (G5665T and T-1370G) and endothelin receptor type A (C+70G and G-231A) in Graves' disease. International Immunopharmacology, 18(1), 198-202. https://doi.org/10.1016/j.intimp.2013.11.017
Aydın AF, et al. Polymorphisms of Endothelin 1 (G5665T and T-1370G) and Endothelin Receptor Type a (C+70G and G-231A) in Graves' Disease. Int Immunopharmacol. 2014;18(1):198-202. PubMed PMID: 24291390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polymorphisms of endothelin 1 (G5665T and T-1370G) and endothelin receptor type A (C+70G and G-231A) in Graves' disease. AU - Aydın,A Fatih, AU - Develi-İş,Seval, AU - Doğru-Abbasoğlu,Semra, AU - Vural,Pervin, AU - Ozderya,Ayşenur, AU - Karadağ,Berrin, AU - Uysal,Müjdat, Y1 - 2013/12/02/ PY - 2013/09/16/received PY - 2013/11/07/revised PY - 2013/11/19/accepted PY - 2013/12/3/entrez PY - 2013/12/3/pubmed PY - 2014/10/2/medline KW - AT KW - Angiogenesis KW - Anti-TPO KW - Anti-Tg KW - Autoantibody KW - CI KW - EDN1 KW - EDNRA KW - Endothelin 1 KW - Endothelin family KW - Endothelin receptor type A KW - GD KW - Graves' disease KW - HWE KW - Hardy–Weinberg Equilibrium KW - ICAM1 KW - Intercellular adhesion molecule 1 KW - MMI KW - OR KW - Odds ratio KW - Ophthalmopathy KW - PTU KW - Polymorphism KW - SNPs KW - TNFα KW - TRAb KW - TSH KW - TSH receptor antibody KW - Tumor necrosis factor α KW - UTR KW - Untranslated region KW - VEGF KW - anti-thyroglobulin KW - anti-thyroid KW - anti-thyroid peroxidase KW - confidence interval KW - methimazole KW - propylthiouracil KW - single nucleotide polymorphisms KW - thyroid-stimulating hormone KW - vascular endothelial growth factor SP - 198 EP - 202 JF - International immunopharmacology JO - Int Immunopharmacol VL - 18 IS - 1 N2 - PURPOSE: Endothelin 1 (EDN1) is a strong angiogenic and mitogenic factor, playing a key role in hypervascularization, thyroid follicle cell hyperplasia, and lymphocyte infiltration in the thyroid gland of patients with Graves' disease (GD). EDN1 induces angiogenesis and mitogenesis via endothelin receptor type A (EDNRA). This study examined the possible association of EDN1 (G5665T and T-1370G) and EDNRA (C+70G and G-231A) single nucleotide polymorphisms (SNPs) with the occurrence of GD, and evaluates the relationship between genotypes and clinical/laboratory manifestations of GD. MATERIALS AND METHODS: We analyzed genotype and allele distributions of EDN1 and EDNRA polymorphisms in 165 patients with GD and 181 healthy controls by real-time PCR combined with melting curve analysis. RESULTS: No significant associations between GD and variant alleles of the studied polymorphisms were observed. However, the anti-thyroid peroxidase (anti-TPO) and anti-thyroglobulin (anti-TG) levels in EDN1 G5665T GG genotype were higher than those in T allele carriers (GT+TT) (p=0.001 and p=0.026, respectively). In addition, anti-TPO levels in EDN1 T-1370G wild-type homozygous patients were found to be higher than in mutant gene carrying patients (GT+GG) (p=0.006). The presence of EDNRA+70G allele was associated with 3.37-fold increased risk for development of ophthalmopathy in GD patients (p=0.009). CONCLUSION: Although there were no associations between EDN1 (G5665T and T-1370G) and EDNRA (C+70G and G-231A) SNPs and susceptibility to GD, EDN1 G5665T and T-1370G polymorphisms were related to alterations of autoantibody production and EDNRA C+70G polymorphism is related with increased risk for ophthalmopathy in GD patients. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/24291390/Polymorphisms_of_endothelin_1__G5665T_and_T_1370G__and_endothelin_receptor_type_A__C+70G_and_G_231A__in_Graves'_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(13)00468-2 DB - PRIME DP - Unbound Medicine ER -